Recombinant Human BACE-1 Protein, CF | R&D Systems 931-AS-050 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human BACE-1 Protein, CF

catalog :

931-AS-050

quantity :

50 ug

price :

432 USD

citations: 9

Reference
James L, Strickland Z, Lopez N, Whited J, Maden M, Lewis J. Identification and Analysis of Axolotl Homologs for Proteins Implicated in Human Neurodegenerative Proteinopathies. Genes (Basel). 2024;15: pubmed publisher
Stern N, Gacs A, T xe1 trai E, Flachner B, Hajd xfa I, Dobi K, et al. Dual Inhibitors of AChE and BACE-1 for Reducing Aβ in Alzheimer's Disease: From In Silico to In Vivo. Int J Mol Sci. 2022;23: pubmed publisher
Kim W, Watanabe H, Lomoio S, Tesco G. Spatiotemporal processing of neural cell adhesion molecules 1 and 2 by BACE1 in vivo. J Biol Chem. 2021;296:100372 pubmed publisher
Zou Y, Hu J, Huang W, Zhu L, Shao M, Dordoe C, et al. The botanical origin and antioxidant, anti-BACE1 and antiproliferative properties of bee pollen from different regions of South Korea. BMC Complement Med Ther. 2020;20:236 pubmed publisher
Koirala S, Klein J, Zheng Y, Glenn N, Eisemann T, Fon Tacer K, et al. Tissue-Specific Regulation of the Wnt/β-Catenin Pathway by PAGE4 Inhibition of Tankyrase. Cell Rep. 2020;32:107922 pubmed publisher
Netzer W, Bettayeb K, Sinha S, Flajolet M, Greengard P, Bustos V. Gleevec shifts APP processing from a Î²-cleavage to a nonamyloidogenic cleavage. Proc Natl Acad Sci U S A. 2017;114:1389-1394 pubmed publisher
Thomas R, Liddell J, Kidd E. Anti-amyloid precursor protein immunoglobulins inhibit amyloid-? production by steric hindrance. FEBS J. 2011;278:167-78 pubmed publisher
Ahmed R, Holler C, Webb R, Li F, Beckett T, Murphy M. BACE1 and BACE2 enzymatic activities in Alzheimer's disease. J Neurochem. 2010;112:1045-53 pubmed publisher
Na C, Jeon S, Zhang G, Olson G, Chae C. Inhibition of amyloid beta-peptide production by blockage of beta-secretase cleavage site of amyloid precursor protein. J Neurochem. 2007;101:1583-95 pubmed

product information

master code :

931-AS

SKU :

931-AS-050

product name :

Recombinant Human BACE-1 Protein, CF

unit size :

50 ug

description :

The Recombinant Human BACE-1 Protein, CF from R&D Systems is derived from NS0. The Recombinant Human BACE-1 Protein, CF has been validated for the following applications: Enzyme Activity.

target :

BACE-1

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS.

conjugate :

Unconjugated

purity :

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Human

observed molecular weight :

68-70 kDa and 65-67 kDa, reducing conditions

theoretical molecular weight :

50 kDa (Pro form) 47 kDa (Mature form)

gene symbol :

BACE1

details of functionality :

Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK(Dpn)RR-NH2 (Catalog # ES004 ). The specific activity is 3.5 pmol/min/╡g, as measured under the described conditions.

endotoxin note :

<1.0 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

P56817.1

applications :

Enzyme Activity

source long :

Mouse myeloma cell line, NS0-derived human BACE-1 protein Thr22-Tyr460 (pro) & Glu46-Tyr460 (mature), both with a C-terminal 10-His tag

source short :

NS0

USD :

432 USD

alt names :

APP beta-secretase, ASP2, Aspartyl protease 2, BACE1, BACEAsp 2, beta-secretase 1, beta-secretase 1 precursor variant 1, beta-site amyloid beta A4 precursor protein-cleaving enzyme, Beta-site amyloid precursor protein cleaving enzyme 1, Beta-site APP cleaving enzyme 1, beta-site APP-cleaving enzyme, beta-site APP-cleaving enzyme 1, EC 3.4.23, EC 3.4.23.46, FLJ90568, HSPC104, KIAA1149, Memapsin-2, Membrane-associated aspartic protease 2, transmembrane aspartic proteinase Asp2

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 ░C as supplied. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.