Recombinant Human Angiopoietin-1 Protein | R&D Systems 923-AN product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Angiopoietin-1 Protein

catalog :

923-AN

quantity :

25 ug

price :

309 USD

citations: 56

Reference
THACKABERRY E, Zhou Y, Zuch de Zafra C, Fuh G, Lee C, Sanowar S, et al. Rapid Development of Glaucoma Via ITV Nonselective ANGPT 1/2 Antibody: A Potential Role for ANGPT/TIE2 Signaling in Primate Aqueous Humor Outflow. Invest Ophthalmol Vis Sci. 2019;60:4097-4108 pubmed publisher
Muraguchi T, Nanba D, Nishimura E, Tashiro T. IGF-1R deficiency in human keratinocytes disrupts epidermal homeostasis and stem cell maintenance. J Dermatol Sci. 2019;: pubmed publisher
Stephenson S, Care M, Fan I, Zougman A, Westhead D, Doody G, et al. Growth Factor-like Gene Regulation Is Separable from Survival and Maturation in Antibody-Secreting Cells. J Immunol. 2019;202:1287-1300 pubmed publisher
Elamaa H, Kihlström M, Kapiainen E, Kaakinen M, Miinalainen I, Ragauskas S, et al. Angiopoietin-4-dependent venous maturation and fluid drainage in the peripheral retina. elife. 2018;7: pubmed publisher
Higgins S, De Ceunynck K, Kellum J, Chen X, Gu X, Chaudhry S, et al. Tie2 protects the vasculature against thrombus formation in systemic inflammation. J Clin Invest. 2018;128:1471-1484 pubmed publisher
Souma T, Thomson B, Heinen S, Carota I, Yamaguchi S, Onay T, et al. Context-dependent functions of angiopoietin 2 are determined by the endothelial phosphatase VEPTP. Proc Natl Acad Sci U S A. 2018;115:1298-1303 pubmed publisher
La Porta S, Roth L, Singhal M, Mogler C, Spegg C, Schieb B, et al. Endothelial Tie1-mediated angiogenesis and vascular abnormalization promote tumor progression and metastasis. J Clin Invest. 2018;128:834-845 pubmed publisher
Daly C, Qian X, Castanaro C, Pasnikowski E, Jiang X, Thomson B, et al. Angiopoietins bind thrombomodulin and inhibit its function as a thrombin cofactor. Sci Rep. 2018;8:505 pubmed publisher
Pan L, Chen Y, Li H, Bi L, Sun W, Sun G, et al. Dachengqi Decoction Attenuates Intestinal Vascular Endothelial Injury in Severe Acute Pancreatitis in Vitro and in Vivo. Cell Physiol Biochem. 2017;44:2395-2406 pubmed publisher
Eich G, Bartosova M, Tischer C, Wlodkowski T, Schaefer B, Pichl S, et al. Bicarbonate buffered peritoneal dialysis fluid upregulates angiopoietin-1 and promotes vessel maturation. PLoS ONE. 2017;12:e0189903 pubmed publisher
Gutbier B, Jiang X, Dietert K, Ehrler C, Lienau J, Van Slyke P, et al. Vasculotide reduces pulmonary hyperpermeability in experimental pneumococcal pneumonia. Crit Care. 2017;21:274 pubmed publisher
Chen J, Luo Y, Hui H, Cai T, Huang H, Yang F, et al. CD146 coordinates brain endothelial cell-pericyte communication for blood-brain barrier development. Proc Natl Acad Sci U S A. 2017;114:E7622-E7631 pubmed publisher
Burnett A, Gomez I, De Leon D, Ariaans M, Progias P, Kammerer R, et al. Angiopoietin-1 enhances neutrophil chemotaxis in vitro and migration in vivo through interaction with CD18 and release of CCL4. Sci Rep. 2017;7:2332 pubmed publisher
Shlamkovich T, Aharon L, Barton W, Papo N. Utilizing combinatorial engineering to develop Tie2 targeting antagonistic angiopoetin-2 ligands as candidates for anti-angiogenesis therapy. Oncotarget. 2017;8:33571-33585 pubmed publisher
Moore J, Lemmon M, Ferguson K. Dimerization of Tie2 mediated by its membrane-proximal FNIII domains. Proc Natl Acad Sci U S A. 2017;114:4382-4387 pubmed publisher
Wang K, Liu W, Song Y, Wu X, Zhang Y, Li S, et al. The role of angiopoietin-2 in nucleus pulposus cells during human intervertebral disc degeneration. Lab Invest. 2017;97:971-982 pubmed publisher
Ho Y, Adriani G, Beyer S, Nhan P, Kamm R, Kah J. A Facile Method to Probe the Vascular Permeability of Nanoparticles in Nanomedicine Applications. Sci Rep. 2017;7:707 pubmed publisher
Morooka N, Futaki S, Sato Nishiuchi R, Nishino M, Totani Y, Shimono C, et al. Polydom Is an Extracellular Matrix Protein Involved in Lymphatic Vessel Remodeling. Circ Res. 2017;120:1276-1288 pubmed publisher
Spicer L, SchÃ¼tz L, Williams J, Schreiber N, Evans J, Totty M, et al. G protein-coupled receptor 34 in ovarian granulosa cells of cattle: changes during follicular development and potential functional implications. Domest Anim Endocrinol. 2017;59:90-99 pubmed publisher
Hossain M, Shifat R, Johnson D, Bedford M, Gabrusiewicz K, Cortes Santiago N, et al. TIE2-mediated tyrosine phosphorylation of H4 regulates DNA damage response by recruiting ABL1. Sci Adv. 2016;2:e1501290 pubmed
Yu S, Yao S, Wen Y, Wang Y, Wang H, Xu Q. Angiogenic microspheres promote neural regeneration and motor function recovery after spinal cord injury in rats. Sci Rep. 2016;6:33428 pubmed publisher
Dumas E, Neagoe P, McDonald P, White M, Sirois M. New Insights into the Pro-Inflammatory Activities of Ang1 on Neutrophils: Induction of MIP-1? Synthesis and Release. PLoS ONE. 2016;11:e0163140 pubmed publisher
Korhonen E, Lampinen A, Giri H, Anisimov A, Kim M, Allen B, et al. Tie1 controls angiopoietin function in vascular remodeling and inflammation. J Clin Invest. 2016;126:3495-510 pubmed publisher
Chan W, Ismail H, Mayaki D, Sanchez V, Tiedemann K, Davis E, et al. Fibulin-5 Regulates Angiopoietin-1/Tie-2 Receptor Signaling in Endothelial Cells. PLoS ONE. 2016;11:e0156994 pubmed publisher
Phanthanawiboon S, Limkittikul K, Sakai Y, Takakura N, Saijo M, Kurosu T. Acute Systemic Infection with Dengue Virus Leads to Vascular Leakage and Death through Tumor Necrosis Factor-α and Tie2/Angiopoietin Signaling in Mice Lacking Type I and II Interferon Receptors. PLoS ONE. 2016;11:e0148564 pubmed publisher
Siddiqui M, Mayanil C, Kim K, Tomita T. Angiopoietin-1 Regulates Brain Endothelial Permeability through PTPN-2 Mediated Tyrosine Dephosphorylation of Occludin. PLoS ONE. 2015;10:e0130857 pubmed publisher
Hakanpaa L, Sipilä T, Leppänen V, Gautam P, Nurmi H, Jacquemet G, et al. Endothelial destabilization by angiopoietin-2 via integrin Î²1 activation. Nat Commun. 2015;6:5962 pubmed publisher
Wang S, Amato K, Song W, Youngblood V, Lee K, Boothby M, et al. Regulation of endothelial cell proliferation and vascular assembly through distinct mTORC2 signaling pathways. Mol Cell Biol. 2015;35:1299-313 pubmed publisher
Korpela E, Yohan D, Chin L, Kim A, Huang X, Sade S, et al. Vasculotide, an Angiopoietin-1 mimetic, reduces acute skin ionizing radiation damage in a preclinical mouse model. BMC Cancer. 2014;14:614 pubmed publisher
Brunckhorst M, Xu Y, Lu R, Yu Q. Angiopoietins promote ovarian cancer progression by establishing a procancer microenvironment. Am J Pathol. 2014;184:2285-96 pubmed publisher
Tahir T, Singh H, Brindle N. The RNA binding protein hnRNP-K mediates post-transcriptional regulation of uncoupling protein-2 by angiopoietin-1. Cell Signal. 2014;26:1379-84 pubmed publisher
Yao J, Cui M, Li M, Liu Y, He Q, Xiao J, et al. Angiopoietin1 inhibits mast cell activation and protects against anaphylaxis. PLoS ONE. 2014;9:e89148 pubmed publisher
Haddad L, Sirois M. Angiopoietin-1 upregulates de novo expression of IL-1? and Il1-Ra, and the exclusive release of Il1-Ra from human neutrophils. PLoS ONE. 2014;9:e88980 pubmed publisher
Grafton K, Moir L, Black J, Hansbro N, Hansbro P, Burgess J, et al. LF-15 & T7, synthetic peptides derived from tumstatin, attenuate aspects of airway remodelling in a murine model of chronic OVA-induced allergic airway disease. PLoS ONE. 2014;9:e85655 pubmed publisher
Pellegrinelli V, Rouault C, Veyrie N, Clement K, Lacasa D. Endothelial cells from visceral adipose tissue disrupt adipocyte functions in a three-dimensional setting: partial rescue by angiopoietin-1. Diabetes. 2014;63:535-49 pubmed publisher
Brindle N, Sale J, Arakawa H, Buerstedde J, Nuamchit T, Sharma S, et al. Directed evolution of an angiopoietin-2 ligand trap by somatic hypermutation and cell surface display. J Biol Chem. 2013;288:33205-12 pubmed publisher
Jin H, Bae Y, Kim M, Kwon S, Jeon H, Choi S, et al. Comparative analysis of human mesenchymal stem cells from bone marrow, adipose tissue, and umbilical cord blood as sources of cell therapy. Int J Mol Sci. 2013;14:17986-8001 pubmed publisher
Yu X, Seegar T, Dalton A, Tzvetkova Robev D, Goldgur Y, Rajashankar K, et al. Structural basis for angiopoietin-1-mediated signaling initiation. Proc Natl Acad Sci U S A. 2013;110:7205-10 pubmed publisher
Yamakawa D, Kidoya H, Sakimoto S, Jia W, Naito H, Takakura N. Ligand-independent Tie2 dimers mediate kinase activity stimulated by high dose angiopoietin-1. J Biol Chem. 2013;288:12469-77 pubmed publisher
Hanjaya Putra D, Shen Y, Wilson A, Fox Talbot K, Khetan S, Burdick J, et al. Integration and regression of implanted engineered human vascular networks during deep wound healing. Stem Cells Transl Med. 2013;2:297-306 pubmed publisher
Straussman R, Morikawa T, Shee K, Barzily Rokni M, Qian Z, Du J, et al. Tumour micro-environment elicits innate resistance to RAF inhibitors through HGF secretion. Nature. 2012;487:500-4 pubmed publisher
David S, Park J, Meurs M, Zijlstra J, Koenecke C, Schrimpf C, et al. Acute administration of recombinant Angiopoietin-1 ameliorates multiple-organ dysfunction syndrome and improves survival in murine sepsis. Cytokine. 2011;55:251-9 pubmed publisher
Brunckhorst M, Wang H, Lu R, Yu Q. Angiopoietin-4 promotes glioblastoma progression by enhancing tumor cell viability and angiogenesis. Cancer Res. 2010;70:7283-93 pubmed publisher
Song H, Suehiro J, Kanki Y, Kawai Y, Inoue K, Daida H, et al. Critical role for GATA3 in mediating Tie2 expression and function in large vessel endothelial cells. J Biol Chem. 2009;284:29109-24 pubmed publisher
Dallabrida S, Ismail N, Pravda E, Parodi E, Dickie R, Durand E, et al. Integrin binding angiopoietin-1 monomers reduce cardiac hypertrophy. FASEB J. 2008;22:3010-23 pubmed publisher
Saharinen P, Eklund L, Miettinen J, Wirkkala R, Anisimov A, Winderlich M, et al. Angiopoietins assemble distinct Tie2 signalling complexes in endothelial cell-cell and cell-matrix contacts. Nat Cell Biol. 2008;10:527-37 pubmed publisher
Rao S, Lobov I, Vallance J, Tsujikawa K, Shiojima I, Akunuru S, et al. Obligatory participation of macrophages in an angiopoietin 2-mediated cell death switch. Development. 2007;134:4449-58 pubmed
Clavel G, Bessis N, Lemeiter D, Fardellone P, Mejjad O, Menard J, et al. Angiogenesis markers (VEGF, soluble receptor of VEGF and angiopoietin-1) in very early arthritis and their association with inflammation and joint destruction. Clin Immunol. 2007;124:158-64 pubmed
Riccioni R, Diverio D, Mariani G, Buffolino S, Riti V, Saulle E, et al. Expression of Tie-2 and other receptors for endothelial growth factors in acute myeloid leukemias is associated with monocytic features of leukemic blasts. Stem Cells. 2007;25:1862-71 pubmed
Ohab J, Fleming S, Blesch A, Carmichael S. A neurovascular niche for neurogenesis after stroke. J Neurosci. 2006;26:13007-16 pubmed
Kalomenidis I, Kollintza A, Sigala I, Papapetropoulos A, Papiris S, Light R, et al. Angiopoietin-2 levels are elevated in exudative pleural effusions. Chest. 2006;129:1259-66 pubmed
Horst A, Ito W, Dabelstein J, Schumacher U, Sander H, Turbide C, et al. Carcinoembryonic antigen-related cell adhesion molecule 1 modulates vascular remodeling in vitro and in vivo. J Clin Invest. 2006;116:1596-605 pubmed
Tsigkos S, Zhou Z, Kotanidou A, Fulton D, Zakynthinos S, Roussos C, et al. Regulation of Ang2 release by PTEN/PI3-kinase/Akt in lung microvascular endothelial cells. J Cell Physiol. 2006;207:506-11 pubmed
Satchell S, Anderson K, Mathieson P. Angiopoietin 1 and vascular endothelial growth factor modulate human glomerular endothelial cell barrier properties. J Am Soc Nephrol. 2004;15:566-74 pubmed
Caine G, Blann A, Stonelake P, Ryan P, Lip G. Plasma angiopoietin-1, angiopoietin-2 and Tie-2 in breast and prostate cancer: a comparison with VEGF and Flt-1. Eur J Clin Invest. 2003;33:883-90 pubmed
Danet G, Pan Y, Luongo J, Bonnet D, Simon M. Expansion of human SCID-repopulating cells under hypoxic conditions. J Clin Invest. 2003;112:126-35 pubmed

product information

brand :

R&D Systems

master code :

923-AN

SKU :

923-AN-025

product name :

Recombinant Human Angiopoietin-1 Protein

description :

The Recombinant Human Angiopoietin-1 Protein from R&D Systems is derived from NS0. The Recombinant Human Angiopoietin-1 Protein has been validated for the following applications: Bioactivity.

target :

Angiopoietin-1

category :

Proteins and Enzymes

unit size :

25 ug

buffer :

Lyophilized from a 0.2 µm filtered solution in Tris-Citrate and NaCl with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

species :

Human

observed molecular weight :

70 kDa, reducing conditions

theoretical molecular weight :

56 kDa

gene symbol :

ANGPT1

details of functionality :

Measured by its ability to inhibit serum deprivation induced apoptosis in HUVEC human umbilical vein endothelial cells. Kwak, H.J. et al . (1999) FEBS Letters 448 :249. The ED50 for this effect is 10-40 ng/mL in the presence of 5 µg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050 ).

endotoxin note :

<1.0 EU per 1 µg of the protein by the LAL method.

catalog number base :

923-AN

accessionNumbers :

Q5HYA0

applications :

Bioactivity

source :

Mouse myeloma cell line, NS0-derived human Angiopoietin-1 protein Ser20-Phe498, with a C-terminal 6-His tag

2020 USD :

309

2021 USD :

309 USD

alt names :

AGP1, AGPT, Ang1, ANG-1, angiopoietin 1, angiopoietin-1, ANGPT1, KIAA0003

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.