Recombinant Porcine IL-12 Protein | R&D Systems 912-PL-025 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Porcine IL-12 Protein

catalog :

912-PL-025

quantity :

25 ug

price :

480 USD

citations: 7

Reference
Hamid B, Schlosser Brandenburg J, Bechtold L, Ebner F, Rausch S, Hartmann S. Early Immune Initiation by Porcine Cells following Toxoplasma gondii Infection versus TLR Ligation. Microorganisms. 2021;9: pubmed publisher
Holzer B, Rijal P, McNee A, Paudyal B, Martini V, Clark B, et al. Protective porcine influenza virus-specific monoclonal antibodies recognize similar haemagglutinin epitopes as humans. PLoS Pathog. 2021;17:e1009330 pubmed publisher
Elahi S, Thompson D, Van Kessel J, Babiuk L, Gerdts V. Protective Role of Passively Transferred Maternal Cytokines against Bordetella pertussis Infection in Newborn Piglets. Infect Immun. 2017;85: pubmed publisher
Mair K, Müllebner A, Essler S, Duvigneau J, Storset A, Saalmuller A, et al. Porcine CD8?dim/-NKp46high NK cells are in a highly activated state. Vet Res. 2013;44:13 pubmed publisher
Mair K, Essler S, Patzl M, Storset A, Saalmuller A, Gerner W. NKp46 expression discriminates porcine NK cells with different functional properties. Eur J Immunol. 2012;42:1261-71 pubmed publisher
Toka F, Nfon C, Dawson H, Estes D, Golde W. Activation of porcine natural killer cells and lysis of foot-and-mouth disease virus infected cells. J Interferon Cytokine Res. 2009;29:179-92 pubmed publisher
Azevedo M, Yuan L, Pouly S, Gonzales A, Jeong K, Nguyen T, et al. Cytokine responses in gnotobiotic pigs after infection with virulent or attenuated human rotavirus. J Virol. 2006;80:372-82 pubmed

product information

master code :

912-PL

SKU :

912-PL-025

product name :

Recombinant Porcine IL-12 Protein

unit size :

25 ug

description :

The Recombinant Porcine IL-12 Protein from R&D Systems is derived from CHO. The Recombinant Porcine IL-12 Protein has been validated for the following applications: Bioactivity.

target :

IL-12

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

species :

Porcine

observed molecular weight :

60-70 kDa, non-reducing conditions

details of functionality :

Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. Symons, J.A. etáal . (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. etáal . (eds): IRL Press. 272. The ED50 for this effect is 0.05-0.15áng/mL.

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

Q28938 (p40) Q29053 (p35)

applications :

Bioactivity

source long :

Chinese Hamster Ovary cell line, CHO-derived porcine IL-12 protein Porcine IL-12 p40 (Ile23 - Asn324) Accession # Q28938 Porcine IL-12 p35 (Arg26 - Ser222) Accession # Q29053 N-terminus C-terminus

source short :

CHO

USD :

480 USD

alt names :

CLMF p35, CLMF1, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL12, IL-12 subunit p35, IL-12, subunit p35, IL12A, interleukin 12, p35, interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35), interleukin-12 alpha chain, interleukin-12 subunit alpha, natural killer cell stimulatory factor 1, 35 kD subunit, NF cell stimulatory factor chain 1, NK cell stimulatory factor chain 1, NKSF1, p35

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.