product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant F. meningosepticum PNGase F Protein, CF
catalog :
9109-GH-020
quantity :
20 ug (also 100 ug, 1 mg)
price :
237 USD
more info or order :
R&D Systems product webpage
citations: 3
Reference
Lusche D, Wessels D, Reis R, Forrest C, Thumann A, Soll D. New monoclonal antibodies that recognize an unglycosylated, conserved, extracellular region of CD44 in vitro and in vivo, and can block tumorigenesis. PLoS ONE. 2021;16:e0250175 pubmed publisher
Alexander C, Martin J, Oxman E, Kasza I, Senn K, Dvinge H. Alternative Splicing and Cleavage of GLUT8. Mol Cell Biol. 2020;41: pubmed publisher
Costello A, Lao N, Barron N, Clynes M. Continuous translation of circularized mRNA improves recombinant protein titer. Metab Eng. 2019;52:284-292 pubmed publisher
image
image 1 :
R&D Systems 9109-GH-020 image 1
Recombinant F. meningosepticum PNGase F Protein Enzyme Activity
product information
brand :
R&D Systems
master code :
9109-GH
SKU :
9109-GH-020
product name :
Recombinant F. meningosepticum PNGase F Protein, CF
description :
The Recombinant F. meningosepticum PNGase F Protein, CF from R&D Systems is derived from E. coli. The Recombinant F. meningosepticum PNGase F Protein, CF has been validated for the following applications: Enzyme Activity.
target :
PNGase F
category :
Proteins and Enzymes
sizes available :
20 ug (also 100 ug, 1 mg)
buffer :
Supplied as a 0.2 µm filtered solution in Tris and NaCl.
conjugate :
Unconjugated
purity :
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
species :
F. meningosepticum
observed molecular weight :
34 kDa, reducing conditions
theoretical molecular weight :
36 kDa
details of functionality :
Measured by its ability to deglycosylate ribonuclease B under denatured conditions. 50% ribonuclease B (10 µg) is deglycosylated by 2.5 ng rFmPNGase F within 30 minutes, as measured under the described conditions.
endotoxin note :
<1.0 EU per 1 µg of the protein by the LAL method.
accessionNumbers :
P21163
applications :
Enzyme Activity
extended description :
Effective removal of N-linked oligosaccharides from glycoproteins. Glycerol-free, suitable for downstream MS and HPLC. Get 50% off! Use Promo Code: PNGaseF50
source :
E. coli-derived Recombinant F. meningosepticum PNGase F Protein, CF
USD :
237
USD 2023 :
237 USD
product details :
Effective removal of N-linked oligosaccharides from glycoproteins. Glycerol-free, suitable for downstream MS and HPLC. Get 50% off! Use Promo Code: PNGaseF50
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.