product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human Sonic Hedgehog/Shh Protein, High Activity
catalog :
8908-SH-005
quantity :
5 ug
price :
400 USD
more info or order :
R&D Systems product webpage
citations: 12
Reference
Ehring K, Ehlers S, Froese J, Gude F, Puschmann J, Grobe K. Two-way Dispatched function in Sonic hedgehog shedding and transfer to high-density lipoproteins. elife. 2024;12: pubmed publisher
Schlissel G, Meziane M, Narducci D, Hansen A, Li P. Diffusion barriers imposed by tissue topology shape Hedgehog morphogen gradients. Proc Natl Acad Sci U S A. 2024;121:e2400677121 pubmed publisher
Shi P, Tian J, Mallinger J, Ling D, Deleyrolle L, McIntyre J, et al. Increasing Ciliary ARL13B Expression Drives Active and Inhibitor-Resistant Smoothened and GLI into Glioma Primary Cilia. Cells. 2023;12: pubmed publisher
Li W, Wang L, Wierbowski B, Lu M, Dong F, Liu W, et al. Structural insights into proteolytic activation of the human Dispatched1 transporter for Hedgehog morphogen release. Nat Commun. 2021;12:6966 pubmed publisher
Gao F, Klinedinst D, Fernandez F, Cheng B, Savonenko A, Devenney B, et al. Forebrain Shh overexpression improves cognitive function and locomotor hyperactivity in an aneuploid mouse model of Down syndrome and its euploid littermates. Acta Neuropathol Commun. 2021;9:137 pubmed publisher
Sun J, Shin D, Eiseman M, Yallowitz A, Li N, Lalani S, et al. SLITRK5 is a negative regulator of hedgehog signaling in osteoblasts. Nat Commun. 2021;12:4611 pubmed publisher
Ichimiya S, Onishi H, Nagao S, Koga S, Sakihama K, Nakayama K, et al. GLI2 but not GLI1/GLI3 plays a central role in the induction of malignant phenotype of gallbladder cancer. Oncol Rep. 2021;45:997-1010 pubmed publisher
Xu Y, Xi J, Wang G, Guo Z, Sun Q, Lu C, et al. PAUPAR and PAX6 sequentially regulate human embryonic stem cell cortical differentiation. Nucleic Acids Res. 2021;49:1935-1950 pubmed publisher
Griffin S, Pickard M, Hawkins C, Williams A, Fricker R. Nicotinamide restricts neural precursor proliferation to enhance catecholaminergic neuronal subtype differentiation from mouse embryonic stem cells. PLoS ONE. 2020;15:e0233477 pubmed publisher
Kim J, Yin X, Jhaldiyal A, Khan M, Martin I, Xie Z, et al. Defects in mRNA Translation in LRRK2-Mutant hiPSC-Derived Dopaminergic Neurons Lead to Dysregulated Calcium Homeostasis. Cell Stem Cell. 2020;27:633-645.e7 pubmed publisher
Qi X, Schmiege P, Coutavas E, Wang J, Li X. Structures of human Patched and its complex with native palmitoylated sonic hedgehog. Nature. 2018;560:128-132 pubmed publisher
Kurio N, Saunders C, Bechtold T, Salhab I, Nah H, Sinha S, et al. Roles of Ihh signaling in chondroprogenitor function in postnatal condylar cartilage. Matrix Biol. 2018;67:15-31 pubmed publisher
product information
master code :
8908-SH
SKU :
8908-SH-005
product name :
Recombinant Human Sonic Hedgehog/Shh Protein, High Activity
unit size :
5 ug
description :
The Recombinant Human Sonic Hedgehog/Shh Protein, High Activity from R&D Systems is derived from HEK293. The Recombinant Human Sonic Hedgehog/Shh Protein, High Activity has been validated for the following applications: Bioactivity.
target :
Sonic Hedgehog/Shh
category :
Proteins and Enzymes
buffer :
Supplied as a 0.2 ╡m filtered solution in MES, NaCl and CHAPS with BSA as a carrier protein.
conjugate :
Unconjugated
purity :
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.
species :
Human
observed molecular weight :
18-24 kDa, reducing conditions
theoretical molecular weight :
20 kDa
gene symbol :
SHH
details of functionality :
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al . (1997) Biochem. Biophys. Res. Commun. 237 :465. The ED50 for this effect is typically 6-36 ng/mL.
endotoxin note :
<0.10 EU per 1 ╡g of the protein by the LAL method.
top caption :
Enhanced Activity of Human Cell-expressed Sonic Hedgehog (Shh) Protein.
accessionNumbers :
Q15465
applications :
Bioactivity
source long :
Human embryonic kidney cell, HEK293-derived human Sonic Hedgehog/Shh protein Cys24-Gly197
source short :
HEK293
extended description :
C-terminal cholesterol, N-terminal fatty acid-modified
USD :
400 USD
product details :
C-terminal cholesterol, N-terminal fatty acid-modified
alt names :
HHG1, HHG-1, HLP3, HPE3, MCOPCB5, MCOPCB5sonic hedgehog (Drosophila) homolog, Shh, ShhNC, SMMCI, SMMCIsonic hedgehog homolog (Drosophila), sonic hedgehog, sonic hedgehog homolog, sonic hedgehog protein, TPT, TPTPS
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 ░C as supplied. 3 months, -20 to -70 ░C under sterile conditions after opening.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.