Recombinant Human BID (Caspase-8-cleaved) Protein, CF | R&D Systems 882-B8 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human BID (Caspase-8-cleaved) Protein, CF

catalog :

882-B8

quantity :

50 ug

price :

369 USD

citations: 5

Reference
Guerra R, Bird G, Harvey E, Dharia N, Korshavn K, Prew M, et al. Precision Targeting of BFL-1/A1 and an ATM Co-dependency in Human Cancer. Cell Rep. 2018;24:3393-3403.e5 pubmed publisher
Andreu Fernández V, Sancho M, Genoves A, Lucendo E, Todt F, Lauterwasser J, et al. Bax transmembrane domain interacts with prosurvival Bcl-2 proteins in biological membranes. Proc Natl Acad Sci U S A. 2017;114:310-315 pubmed publisher
Lauterwasser J, Todt F, Zerbes R, Nguyen T, Craigen W, Lazarou M, et al. The porin VDAC2 is the mitochondrial platform for Bax retrotranslocation. Sci Rep. 2016;6:32994 pubmed publisher
Shulga N, Pastorino J. Acyl coenzyme A-binding protein augments bid-induced mitochondrial damage and cell death by activating mu-calpain. J Biol Chem. 2006;281:30824-33 pubmed
Liu F, Goff L, Hao J, Newland A, Jia L. Increase in the ratio of mitochondrial Bax/Bcl-XL induces Bax activation in human leukemic K562 cell line. Apoptosis. 2004;9:377-84 pubmed

product information

brand :

R&D Systems

master code :

882-B8

SKU :

882-B8-050

product name :

Recombinant Human BID (Caspase-8-cleaved) Protein, CF

description :

The Recombinant Human BID (Caspase-8-cleaved) Protein, CF from R&D Systems is derived from E. coli. The Recombinant Human BID (Caspase-8-cleaved) Protein, CF has been validated for the following applications: Enzyme Activity.

target :

BID

category :

Proteins and Enzymes

unit size :

50 ug

buffer :

Supplied as a 0.2 µm filtered solution in HEPES-KOH and KCl.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

species :

Human

observed molecular weight :

9 kDa 14 kDa, reducing conditions

theoretical molecular weight :

7 kDa (N-terminal fragment) 15 kDa (C-terminal fragment)

gene symbol :

BID

details of functionality :

Measured by its ability to induce cytochrome c release from isolated mouse liver mitochondria using the Rat/Mouse Cytochrome c Quantikine ELISA (Catalog # MCTC0 ) to quantify cytochrome c. The typical EC50 for cytochrome c releasing activity is 50 nM. The EC50 for the desired application should be determined. Uncleaved Recombinant Human BID (Catalog # 846-BD ) is available.

catalog number base :

882-B8

accessionNumbers :

P55957

applications :

Enzyme Activity

source :

E. coli -derived human BID protein Met1-Asp195, protein was purified, cleaved by Caspase-8 and further purified

2020 USD :

369

2021 USD :

369 USD

alt names :

apoptic death agonist, BH3 interacting domain death agonist, BH3-interacting domain death agonist, BID isoform ES(1b), BID isoform L(2), BID isoform Si6, desmocollin type 4, FP497, Human BID coding sequence, MGC15319, MGC42355, p22 BID, tbid

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.