Recombinant Human Bcl-2 (minus C-Terminus) Protein, CF | R&D Systems 827-BC-050 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Bcl-2 (minus C-Terminus) Protein, CF

catalog :

827-BC-050

quantity :

50 ug

price :

421 USD

more info or order :

R&D Systems product webpage

citations: 3

Reference
Han C, Sim S, Kim S, Singh R, Hwang S, Kim Y, et al. Desensitized chimeric antigen receptor T cells selectively recognize target cells with enhanced antigen expression. Nat Commun. 2018;9:468 pubmed publisher
Iwata A, De Claro R, Morgan Stevenson V, Tupper J, Schwartz B, Liu L, et al. Extracellular administration of BCL2 protein reduces apoptosis and improves survival in a murine model of sepsis. PLoS ONE. 2011;6:e14729 pubmed publisher
Ghosh J, Shenoy A, Clark J. Interactions between important regulatory proteins and human alphaB crystallin. Biochemistry. 2007;46:6308-17 pubmed

product information

brand :

R&D Systems

master code :

827-BC

SKU :

827-BC-050

product name :

Recombinant Human Bcl-2 (minus C-Terminus) Protein, CF

unit size :

50 ug

target :

Bcl-2

category :

Proteins and Enzymes

alt names :

apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, Bcl2, Bcl-2

buffer :

Supplied as a 0.2 µm filtered solution in HEPES, KCl, DTT and Glycerol.

conjugate :

Unconjugated

all available conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

species :

Human

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

observed molecular weight :

27 kDa, under reducing and non-reducing conditions

theoretical molecular weight :

24.5 kDa

gene symbol :

BCL2

details of functionality :

Measured by its ability to inhibit Caspase-8-cleaved BID-mediated release of cytochrome c from isolated mouse liver mitochondria using the Rat/Mouse Cytochrome c Quantikine ELISA (Catalog # MCTC0 ) to quantify cytochrome c. The typical EC50 is less than 500 nM in the presence of 54 nM Recombinant Human BID Caspase-8-cleaved (Catalog # 882-B8 ). Optimal dilutions should be determined by each laboratory for each application.

endotoxin note :

<0.01 EU per 1 µg of the protein by the LAL method.

accessionNumbers :

P10415

applications :

Inhibition Activity

source :

E. coli-derived Recombinant Human Bcl-2 (minus C-Terminus) Protein, CF

USD :

389

Jul-22 :

421 USD

sizes available :

50 ug

more info or order :

R&D Systems product webpage