product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human Lgr5/GPR49 Fc Chimera Protein, CF
catalog :
8078-GP-050
quantity :
50 ug
price :
460 USD
more info or order :
R&D Systems product webpage
citations: 1
Reference
Mileto S, Jardé T, Childress K, Jensen J, Rogers A, Kerr G, et al. Clostridioides difficile infection damages colonic stem cells via TcdB, impairing epithelial repair and recovery from disease. Proc Natl Acad Sci U S A. 2020;117:8064-8073 pubmed publisher
product information
brand :
R&D Systems
master code :
8078-GP
SKU :
8078-GP-050
product name :
Recombinant Human Lgr5/GPR49 Fc Chimera Protein, CF
description :
The Recombinant Human Lgr5/GPR49 Fc Chimera Protein, CF from R&D Systems is derived from CHO. The Recombinant Human Lgr5/GPR49 Fc Chimera Protein, CF has been validated for the following applications: Bioactivity.
target :
Lgr5/GPR49
category :
Proteins and Enzymes
sizes available :
50 ug
buffer :
Lyophilized from a 0.2 µm filtered solution in PBS.
conjugate :
Unconjugated
purity :
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
species :
Human
observed molecular weight :
90-110 kDa, reducing conditions
theoretical molecular weight :
87 kDa (monomer)
gene symbol :
LGR5
details of functionality :
Measured by its binding ability in a functional ELISA. When Recombinant Human R-Spondin 3 (Catalog # 3500-RS/CF ) is coated at 0.5 µg/mL, Recombinant Human Lgr5/GPR49 Fc Chimera binds with an apparent Kd 0.1 nM. Measured by its ability to inhibit Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 1.5-7.5 µg/mL in the presence of 20 ng/mL Recombinant Human R-Spondin 2 (Catalog # 3266-RS ) and 5 ng/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN ).
endotoxin note :
<0.10 EU per 1 µg of the protein by the LAL method.
accessionNumbers :
O75473
applications :
Bioactivity
source :
CHO-derived Recombinant Human Lgr5/GPR49 Fc Chimera Protein, CF
USD :
453
USD 2023 :
460 USD
alt names :
FEX, G protein-coupled receptor 49, GPR49, GPR49G-protein coupled receptor HG38, GPR67, G-protein coupled receptor 49, G-protein coupled receptor 67, GRP49, HG38, leucine-rich repeat containing G protein-coupled receptor 5, leucine-rich repeat-containing G protein-coupled receptor 5, leucine-rich repeat-containing G-protein coupled receptor 5, MGC117008, orphan G protein-coupled receptor HG38
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.