Recombinant Human Lgr5/GPR49 Fc Chimera Protein, CF | R&D Systems 8078-GP-050 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Lgr5/GPR49 Fc Chimera Protein, CF

catalog :

8078-GP-050

quantity :

50 ug

price :

523 USD

citations: 1

Reference
Mileto S, Jardé T, Childress K, Jensen J, Rogers A, Kerr G, et al. Clostridioides difficile infection damages colonic stem cells via TcdB, impairing epithelial repair and recovery from disease. Proc Natl Acad Sci U S A. 2020;117:8064-8073 pubmed publisher

product information

master code :

8078-GP

SKU :

8078-GP-050

product name :

Recombinant Human Lgr5/GPR49 Fc Chimera Protein, CF

unit size :

50 ug

description :

The Recombinant Human Lgr5/GPR49 Fc Chimera Protein, CF from R&D Systems is derived from CHO. The Recombinant Human Lgr5/GPR49 Fc Chimera Protein, CF has been validated for the following applications: Bioactivity.

target :

Lgr5/GPR49

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS.

conjugate :

Unconjugated

purity :

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

species :

Human

observed molecular weight :

90-110 kDa, reducing conditions

theoretical molecular weight :

87 kDa (monomer)

gene symbol :

LGR5

details of functionality :

Measured by its binding ability in a functional ELISA. When Recombinant Human R-Spondin 3 (Catalog # 3500-RS/CF ) is coated at 0.5 ╡g/mL, Recombinant Human Lgr5/GPR49 Fc Chimera binds with an apparent Kd 0.1 nM. Measured by its ability to inhibit Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 1.5-7.5 ╡g/mL in the presence of 20 ng/mL Recombinant Human R-Spondin 2 (Catalog # 3266-RS ) and 5 ng/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN ).

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

O75473

applications :

Bioactivity

source long :

Chinese Hamster Ovary cell line, CHO-derived human Lgr5/GPR49 protein Human LGR-5/GPR49 (Gly22-Ile560) Accession # O75473 IEGRMD Human IgG1 (Pro100-Lys330) N-terminus C-terminus

source short :

CHO

USD :

523 USD

alt names :

FEX, G protein-coupled receptor 49, GPR49, GPR49G-protein coupled receptor HG38, GPR67, G-protein coupled receptor 49, G-protein coupled receptor 67, GRP49, HG38, leucine-rich repeat containing G protein-coupled receptor 5, leucine-rich repeat-containing G protein-coupled receptor 5, leucine-rich repeat-containing G-protein coupled receptor 5, MGC117008, orphan G protein-coupled receptor HG38

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.