product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein
catalog :
788-G8-010
quantity :
10 ug (also 100 ug, 250 ug)
price :
420 USD
more info or order :
citations: 41
Reference
Mishra R, Jha R, Mishra B, Kim Y. Maternal immunization against myostatin suppresses post-hatch chicken growth. PLoS ONE. 2022;17:e0275753 pubmed publisher
Sung D, Kim H, Park S, Lee J, Kim J, Park Y, et al. A New Method of Myostatin Inhibition in Mice via Oral Administration of Lactobacillus casei Expressing Modified Myostatin Protein, BLS-M22. Int J Mol Sci. 2022;23: pubmed publisher
Hagg A, Kharoud S, Goodchild G, Goodman C, Chen J, Thomson R, et al. TMEPAI/PMEPA1 Is a Positive Regulator of Skeletal Muscle Mass. Front Physiol. 2020;11:560225 pubmed publisher
Chang Y, Liu H, Chan Y, Hu S, Liu M, Chang S. The green tea polyphenol epigallocatechin-3-gallate attenuates age-associated muscle loss via regulation of miR-486-5p and myostatin. Arch Biochem Biophys. 2020;692:108511 pubmed publisher
Goossens E, De Vries M, Jukema J, Quax P, Nossent A. Myostatin Inhibits Vascular Smooth Muscle Cell Proliferation and Local 14q32 microRNA Expression, But Not Systemic Inflammation or Restenosis. Int J Mol Sci. 2020;21: pubmed publisher
Kodaka M, Mao F, Arimoto Matsuzaki K, Kitamura M, Xu X, Yang Z, et al. Characterization of a novel compound that promotes myogenesis via Akt and transcriptional co-activator with PDZ-binding motif (TAZ) in mouse C2C12 cells. PLoS ONE. 2020;15:e0231265 pubmed publisher
Dagbay K, Treece E, Streich F, Jackson J, Faucette R, Nikiforov A, et al. Structural basis of specific inhibition of extracellular activation of pro- or latent myostatin by the monoclonal antibody SRK-015. J Biol Chem. 2020;295:5404-5418 pubmed publisher
Liu X, Pan J, Bauman W, Cardozo C. AdipoRon prevents myostatin-induced upregulation of fatty acid synthesis and downregulation of insulin activity in a mouse hepatocyte line. Physiol Rep. 2019;7:e14152 pubmed publisher
Jin Q, Qiao C, Li J, Xiao B, Li J, Xiao X. A GDF11/myostatin inhibitor, GDF11 propeptide-Fc, increases skeletal muscle mass and improves muscle strength in dystrophic mdx mice. Skelet Muscle. 2019;9:16 pubmed publisher
Kim J, Kim J, Sutikno L, Lee S, Jin D, Hong Y, et al. Identification of the minimum region of flatfish myostatin propeptide (Pep45-65) for myostatin inhibition and its potential to enhance muscle growth and performance in animals. PLoS ONE. 2019;14:e0215298 pubmed publisher
Yoon J, Hwang S, Kim M, Lee G, Jeon Y, Hyun S. GDF8 enhances SOX2 expression and blastocyst total cell number in porcine IVF embryo development. Theriogenology. 2019;129:70-76 pubmed publisher
Iskenderian A, Liu N, Deng Q, Huang Y, Shen C, Palmieri K, et al. Myostatin and activin blockade by engineered follistatin results in hypertrophy and improves dystrophic pathology in mdx mouse more than myostatin blockade alone. Skelet Muscle. 2018;8:34 pubmed publisher
Estrellas K, Chung L, Cheu L, Sadtler K, Majumdar S, Mula J, et al. Biological scaffold-mediated delivery of myostatin inhibitor promotes a regenerative immune response in an animal model of Duchenne muscular dystrophy. J Biol Chem. 2018;293:15594-15605 pubmed publisher
Lessard S, Macdonald T, Pathak P, Han M, Coffey V, Edge J, et al. JNK regulates muscle remodeling via myostatin/SMAD inhibition. Nat Commun. 2018;9:3030 pubmed publisher
Formicola L, Pannérec A, Correra R, Gayraud Morel B, Ollitrault D, Besson V, et al. Inhibition of the Activin Receptor Type-2B Pathway Restores Regenerative Capacity in Satellite Cell-Depleted Skeletal Muscle. Front Physiol. 2018;9:515 pubmed publisher
Wu L, Zhu D, Wang B, Lu Y, He P, Zhang Y, et al. Relative abundance of mature myostatin rather than total myostatin is negatively associated with bone mineral density in Chinese. J Cell Mol Med. 2018;22:1329-1336 pubmed publisher
Samant S, Kanwal A, Pillai V, Bao R, Gupta M. The histone deacetylase SIRT6 blocks myostatin expression and development of muscle atrophy. Sci Rep. 2017;7:11877 pubmed publisher
Chen J, Walton K, Hagg A, Colgan T, Johnson K, Qian H, et al. Specific targeting of TGF-β family ligands demonstrates distinct roles in the regulation of muscle mass in health and disease. Proc Natl Acad Sci U S A. 2017;114:E5266-E5275 pubmed publisher
Zhao Y, Liu G, Zambito F, Zhang Y, Desilva B, Kozhich A, et al. A multiplexed immunocapture liquid chromatography tandem mass spectrometry assay for the simultaneous measurement of myostatin and GDF-11 in rat serum using an automated sample preparation platform. Anal Chim Acta. 2017;979:36-44 pubmed publisher
Qin Y, Peng Y, Zhao W, Pan J, Ksiezak Reding H, Cardozo C, et al. Myostatin inhibits osteoblastic differentiation by suppressing osteocyte-derived exosomal microRNA-218: A novel mechanism in muscle-bone communication. J Biol Chem. 2017;292:11021-11033 pubmed publisher
Latres E, Mastaitis J, Fury W, Miloscio L, Trejos J, Pangilinan J, et al. Activin A more prominently regulates muscle mass in primates than does GDF8. Nat Commun. 2017;8:15153 pubmed publisher
Lee S, Park S, Kim Y. Maltose binding protein-fusion enhances the bioactivity of truncated forms of pig myostatin propeptide produced in E. coli. PLoS ONE. 2017;12:e0174956 pubmed publisher
Hammers D, Merscham Banda M, Hsiao J, ENGST S, Hartman J, Sweeney H. Supraphysiological levels of GDF11 induce striated muscle atrophy. EMBO Mol Med. 2017;9:531-544 pubmed publisher
Walker R, Czepnik M, Goebel E, McCoy J, Vujic A, Cho M, et al. Structural basis for potency differences between GDF8 and GDF11. BMC Biol. 2017;15:19 pubmed publisher
Kocsis T, Trencsenyi G, Szabó K, Baán J, Müller G, Mendler L, et al. Myostatin propeptide mutation of the hypermuscular Compact mice decreases the formation of myostatin and improves insulin sensitivity. Am J Physiol Endocrinol Metab. 2017;312:E150-E160 pubmed publisher
Latres E, Pangilinan J, Miloscio L, Bauerlein R, Na E, Potocky T, et al. Myostatin blockade with a fully human monoclonal antibody induces muscle hypertrophy and reverses muscle atrophy in young and aged mice. Skelet Muscle. 2015;5:34 pubmed publisher
Seto D, Kandarian S, Jackman R. A Key Role for Leukemia Inhibitory Factor in C26 Cancer Cachexia. J Biol Chem. 2015;290:19976-86 pubmed publisher
Horbelt D, Boergermann J, Chaikuad A, Alfano I, Williams E, Lukonin I, et al. Small molecules dorsomorphin and LDN-193189 inhibit myostatin/GDF8 signaling and promote functional myoblast differentiation. J Biol Chem. 2015;290:3390-404 pubmed publisher
Relizani K, Mouisel E, Giannesini B, Hourde C, Patel K, Morales Gonzalez S, et al. Blockade of ActRIIB signaling triggers muscle fatigability and metabolic myopathy. Mol Ther. 2014;22:1423-1433 pubmed publisher
Biesemann N, Mendler L, Wietelmann A, Hermann S, Schäfers M, Kruger M, et al. Myostatin regulates energy homeostasis in the heart and prevents heart failure. Circ Res. 2014;115:296-310 pubmed publisher
Yang Z, Nakagawa K, Sarkar A, Maruyama J, Iwasa H, Bao Y, et al. Screening with a novel cell-based assay for TAZ activators identifies a compound that enhances myogenesis in C2C12 cells and facilitates muscle repair in a muscle injury model. Mol Cell Biol. 2014;34:1607-21 pubmed publisher
Rodgers B, Wiedeback B, Hoversten K, Jackson M, Walker R, Thompson T. Myostatin stimulates, not inihibits, C2C12 myoblast proliferation. Endocrinology. 2014;155:670-5 pubmed publisher
Sneddon J, Borowiak M, Melton D. Self-renewal of embryonic-stem-cell-derived progenitors by organ-matched mesenchyme. Nature. 2012;491:765-8 pubmed publisher
Wilson T, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, et al. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature. 2012;487:505-9 pubmed publisher
Diel P, Schiffer T, Geisler S, Hertrampf T, Mosler S, Schulz S, et al. Analysis of the effects of androgens and training on myostatin propeptide and follistatin concentrations in blood and skeletal muscle using highly sensitive immuno PCR. Mol Cell Endocrinol. 2010;330:1-9 pubmed publisher
Wilkes J, Lloyd D, Gekakis N. Loss-of-function mutation in myostatin reduces tumor necrosis factor alpha production and protects liver against obesity-induced insulin resistance. Diabetes. 2009;58:1133-43 pubmed publisher
Sengle G, Ono R, Lyons K, Bachinger H, Sakai L. A new model for growth factor activation: type II receptors compete with the prodomain for BMP-7. J Mol Biol. 2008;381:1025-39 pubmed publisher
Sengle G, Charbonneau N, Ono R, Sasaki T, Alvarez J, Keene D, et al. Targeting of bone morphogenetic protein growth factor complexes to fibrillin. J Biol Chem. 2008;283:13874-88 pubmed publisher
Miller T, Kim S, Yamanaka K, Hester M, Umapathi P, Arnson H, et al. Gene transfer demonstrates that muscle is not a primary target for non-cell-autonomous toxicity in familial amyotrophic lateral sclerosis. Proc Natl Acad Sci U S A. 2006;103:19546-51 pubmed
Ohsawa Y, Hagiwara H, Nakatani M, Yasue A, Moriyama K, Murakami T, et al. Muscular atrophy of caveolin-3-deficient mice is rescued by myostatin inhibition. J Clin Invest. 2006;116:2924-34 pubmed
Amthor H, Nicholas G, McKinnell I, Kemp C, Sharma M, Kambadur R, et al. Follistatin complexes Myostatin and antagonises Myostatin-mediated inhibition of myogenesis. Dev Biol. 2004;270:19-30 pubmed
product information
master code :
788-G8
SKU :
788-G8-010
product name :
Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein
unit size :
10 ug (also 100 ug, 250 ug)
description :
The Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein from R&D Systems is derived from NS0. The Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein has been validated for the following applications: Bioactivity.
target :
GDF-8/Myostatin
category :
Proteins and Enzymes
buffer :
Lyophilized from a 0.2 ╡m filtered solution in Acetonitrile and TFA with BSA as a carrier protein. *1 mg pack size (01M) is supplied as a 0.2 ╡m filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
conjugate :
Unconjugated
purity :
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
species :
Human, Mouse, Rat
observed molecular weight :
12 kDa, reducing conditions 24 kDa, non-reducing conditions
theoretical molecular weight :
12.4 kDa
gene symbol :
MSTN
details of functionality :
Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. etáal . (1991) Method Enzymol. 198 :340. The ED50 for this effect is 2-10 ng/mL.
endotoxin note :
<0.10 EU per 1 ╡g of the protein by the LAL method.
top caption :
Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein Bioactivity
accessionNumbers :
O08689
applications :
Bioactivity
source long :
Mouse myeloma cell line, NS0-derived GDF-8/Myostatin protein Asp268-Ser376
source short :
NS0
USD :
420 USD
alt names :
GDF8, GDF-8, GDF8growth differentiation factor 8, growth/differentiation factor 8, MSLHP, MSTN, Myostatin
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.