Recombinant Human Sirtuin 1/SIRT1 Protein, CF | R&D Systems 7714-DA product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Sirtuin 1/SIRT1 Protein, CF

catalog :

7714-DA

quantity :

50 ug

price :

339 USD

citations: 2

Reference
Jarrett S, Carter K, Bautista R, He D, Wang C, D Orazio J. Sirtuin 1-mediated deacetylation of XPA DNA repair protein enhances its interaction with ATR protein and promotes cAMP-induced DNA repair of UV damage. J Biol Chem. 2018;293:19025-19037 pubmed publisher
Mohibi S, Srivastava S, Bele A, Mirza S, Band H, Band V. Acetylation of Mammalian ADA3 Is Required for Its Functional Roles in Histone Acetylation and Cell Proliferation. Mol Cell Biol. 2016;36:2487-502 pubmed publisher

product information

brand :

R&D Systems

master code :

7714-DA

SKU :

7714-DA-050

product name :

Recombinant Human Sirtuin 1/SIRT1 Protein, CF

description :

The Recombinant Human Sirtuin 1/SIRT1 Protein, CF from R&D Systems is derived from E. coli. The Recombinant Human Sirtuin 1/SIRT1 Protein, CF has been validated for the following applications: Enzyme Activity.

target :

Sirtuin 1/SIRT1

category :

Proteins and Enzymes

unit size :

50 ug

buffer :

Supplied as a 0.2 µm filtered solution in Tris, NaCl, DTT and Glycerol.

conjugate :

Unconjugated

purity :

>75%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.

species :

Human

observed molecular weight :

110-113 kDa, reducing conditions

theoretical molecular weight :

82 kDa

gene symbol :

SIRT1

details of functionality :

Measured by its ability to remove the acetyl group from a fluorogenic peptide substrate Ac-RGK(Ac)-AMC (Catalog # ES016 ) in a coupled assay. The specific activity is 15 pmol/min/µg, as measured under the described conditions.

endotoxin note :

<1.0 EU per 1 µg of the protein by the LAL method.

catalog number base :

7714-DA

accessionNumbers :

Q96EB6

applications :

Enzyme Activity

source :

E. coli -derived human Sirtuin 1/SIRT1 protein Met1-Ser747, with a C-terminal 6-His tag.

2020 USD :

339

2021 USD :

339 USD

alt names :

EC 3.5.1, S. cerevisiae, homolog) 1, SIR2alpha, SIR2L1, SIR2-like protein 1, SIRT1, sirtuin 1

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.