Recombinant Human Sirtuin 1/SIRT1 Protein, CF | R&D Systems 7714-DA-050 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Sirtuin 1/SIRT1 Protein, CF

catalog :

7714-DA-050

quantity :

50 ug

price :

432 USD

more info or order :

R&D Systems product webpage

citations: 2

Reference
Jarrett S, Carter K, Bautista R, He D, Wang C, D Orazio J. Sirtuin 1-mediated deacetylation of XPA DNA repair protein enhances its interaction with ATR protein and promotes cAMP-induced DNA repair of UV damage. J Biol Chem. 2018;293:19025-19037 pubmed publisher
Mohibi S, Srivastava S, Bele A, Mirza S, Band H, Band V. Acetylation of Mammalian ADA3 Is Required for Its Functional Roles in Histone Acetylation and Cell Proliferation. Mol Cell Biol. 2016;36:2487-502 pubmed publisher

product information

master code :

7714-DA

SKU :

7714-DA-050

product name :

Recombinant Human Sirtuin 1/SIRT1 Protein, CF

unit size :

50 ug

description :

The Recombinant Human Sirtuin 1/SIRT1 Protein, CF from R&D Systems is derived from E. coli. The Recombinant Human Sirtuin 1/SIRT1 Protein, CF has been validated for the following applications: Enzyme Activity.

target :

Sirtuin 1/SIRT1

category :

Proteins and Enzymes

buffer :

Supplied as a 0.2 ╡m filtered solution in Tris, NaCl, DTT and Glycerol.

conjugate :

Unconjugated

purity :

>75%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Human

observed molecular weight :

110-113 kDa, reducing conditions

theoretical molecular weight :

82 kDa

gene symbol :

SIRT1

details of functionality :

Measured by its ability to remove the acetyl group from a fluorogenic peptide substrate Ac-RGK(Ac)-AMC (Catalog # ES016 ) in a coupled assay. The specific activity is 15 pmol/min/╡g, as measured under the described conditions.

endotoxin note :

<1.0 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

Q96EB6

applications :

Enzyme Activity

source long :

E. coli -derived human Sirtuin 1/SIRT1 protein Met1-Ser747, with a C-terminal 6-His tag.

source short :

E. coli

USD :

432 USD

alt names :

EC 3.5.1, S. cerevisiae, homolog) 1, SIR2alpha, SIR2L1, SIR2-like protein 1, SIRT1, sirtuin 1

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 ░C as supplied. 3 months, -70 ░C under sterile conditions after opening.

more info or order :

R&D Systems product webpage