product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human Active Heparanase/HPSE Protein, CF
catalog :
7570-GH-005
quantity :
5 ug
price :
560 USD
more info or order :
R&D Systems product webpage
citations: 13
Reference
Manseur C, Groult H, Porta M, Bodet P, Mersni Achour R, Petit R, et al. A Screening Approach to Assess the Impact of Various Commercial Sources of Crude Marine λ-Carrageenan on the Production of Oligosaccharides with Anti-heparanase and Anti-migratory Activities. Mar Drugs. 2023;21: pubmed publisher
He P, Zhang X, Xia K, Green D, Baytas S, Xu Y, et al. Chemoenzymatic synthesis of sulfur-linked sugar polymers as heparanase inhibitors. Nat Commun. 2022;13:7438 pubmed publisher
Cousin R, Groult H, Manseur C, Ferru Cl xe9 ment R, Gani M, Havret R, et al. A Marine λ-Oligocarrageenan Inhibits Migratory and Invasive Ability of MDA-MB-231 Human Breast Cancer Cells through Actions on Heparanase Metabolism and MMP-14/MMP-2 Axis. Mar Drugs. 2021;19: pubmed publisher
Queisser K, Mellema R, Middleton E, Portier I, Manne B, Denorme F, et al. COVID-19 generates hyaluronan fragments that directly induce endothelial barrier dysfunction. JCI Insight. 2021;6: pubmed publisher
Garcia J, McCarthy H, Kuiper J, Melrose J, Roberts S. Perlecan in the Natural and Cell Therapy Repair of Human Adult Articular Cartilage: Can Modifications in This Proteoglycan Be a Novel Therapeutic Approach?. Biomolecules. 2021;11: pubmed publisher
Ko K, Suzuki T, Ishikawa R, Hattori N, Ito R, Umehara K, et al. Ischemic stroke disrupts the endothelial glycocalyx through activation of proHPSE via acrolein exposure. J Biol Chem. 2020;295:18614-18624 pubmed publisher
Njau F, Shushakova N, Schenk H, Wulfmeyer V, Bollin R, Menne J, et al. Calcium dobesilate reduces VEGF signaling by interfering with heparan sulfate binding site and protects from vascular complications in diabetic mice. PLoS ONE. 2020;15:e0218494 pubmed publisher
Kiyan Y, Tkachuk S, Kurselis K, Shushakova N, Stahl K, Dawodu D, et al. Heparanase-2 protects from LPS-mediated endothelial injury by inhibiting TLR4 signalling. Sci Rep. 2019;9:13591 pubmed publisher
Wu Z, Person A, Anderson M, Burroughs B, Tatge T, Khatri K, et al. Imaging specific cellular glycan structures using glycosyltransferases via click chemistry. Glycobiology. 2017;: pubmed publisher
Poupard N, Badarou P, Fasani F, Groult H, Bridiau N, Sannier F, et al. Assessment of Heparanase-Mediated Angiogenesis Using Microvascular Endothelial Cells: Identification of λ-Carrageenan Derivative as a Potent Anti Angiogenic Agent. Mar Drugs. 2017;15: pubmed publisher
Poupard N, Groult H, Bodin J, Bridiau N, Bordenave Juchereau S, Sannier F, et al. Production of heparin and ?-carrageenan anti-heparanase derivatives using a combination of physicochemical depolymerization and glycol splitting. Carbohydr Polym. 2017;166:156-165 pubmed publisher
Bohdan N, Espín S, Águila S, Teruel Montoya R, Vicente V, Corral J, et al. Heparanase Activates Antithrombin through the Binding to Its Heparin Binding Site. PLoS ONE. 2016;11:e0157834 pubmed publisher
Tinholt M, Stavik B, Louch W, Carlson C, Sletten M, Ruf W, et al. Syndecan-3 and TFPI colocalize on the surface of endothelial-, smooth muscle-, and cancer cells. PLoS ONE. 2015;10:e0117404 pubmed publisher
product information
master code :
7570-GH
SKU :
7570-GH-005
product name :
Recombinant Human Active Heparanase/HPSE Protein, CF
unit size :
5 ug
description :
The Recombinant Human Active Heparanase/HPSE Protein, CF from R&D Systems is derived from CHO. The Recombinant Human Active Heparanase/HPSE Protein, CF has been validated for the following applications: Enzyme Activity.
target :
Heparanase/HPSE
category :
Proteins and Enzymes
buffer :
Supplied as a 0.2 ╡m filtered solution in Tris, NaCl and E64.
conjugate :
Unconjugated
purity :
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.
species :
Human
observed molecular weight :
50-60 kDa 7-9 kDa, reducing conditions
theoretical molecular weight :
43 kDa 9 kDa
gene symbol :
HPSE
details of functionality :
Measured by its ability to release biotinylated heparan sulfate from Recombinant Human Syndecan-4 (Catalog # 2918-SD ). 20 ng of Recombinant Human Active Heparanase/HPSE digestion will result in 50% of OD reduction compared with the Negative Control, as measured under the described conditions.
endotoxin note :
<1.0 EU per 1 ╡g of the protein by the LAL method.
accessionNumbers :
AF144325
applications :
Enzyme Activity
source long :
Chinese Hamster Ovary cell line, CHO-derived human Heparanase/HPSE protein Gln36-Ile543 with N-terminal 6-His tag The linker peptide from Ser110-Gln157 is removed by protease treatment
source short :
CHO
USD :
560 USD
alt names :
EC 3.2, Endo-glucoronidase, HEP, heparanase, Heparanase-1, Hpa1, HPAheparanase exon 9 and 10 deletion, HPR1, HPSE, HPSE1heparanase-1, HSE1, HSE1heparanase exon10-deletion
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 ░C as supplied. 3 months, -20 to -70 ░C under sterile conditions after opening.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.