Recombinant Human SPINK1 Protein, CF | R&D Systems 7496-PI-010 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human SPINK1 Protein, CF

catalog :

7496-PI-010

quantity :

10 ug

price :

541 USD

citations: 2

Reference
Luo D, Liu D, Rao C, Shi S, Zeng X, Liu S, et al. Raised SPINK1 levels play a role in angiogenesis and the transendothelial migration of ALL cells. Sci Rep. 2022;12:2999 pubmed publisher
Kim K, Turner R, Khazan N, Kodza A, Jones A, Singh R, et al. Role of trypsin and protease-activated receptor-2 in ovarian cancer. PLoS ONE. 2020;15:e0232253 pubmed publisher

product information

master code :

7496-PI

SKU :

7496-PI-010

product name :

Recombinant Human SPINK1 Protein, CF

unit size :

10 ug

description :

The Recombinant Human SPINK1 Protein, CF from R&D Systems is derived from E. coli. The Recombinant Human SPINK1 Protein, CF has been validated for the following applications: Enzyme Activity.

target :

SPINK1

category :

Proteins and Enzymes

buffer :

Supplied as a 0.2 ╡m filtered solution in MES, NaCl, CaCl2, DTT, Glycerol and Brij-35.

conjugate :

Unconjugated

purity :

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie« Blue stain at 5 ╡g per lane.

species :

Human

observed molecular weight :

8-11 kDa, reducing conditions

theoretical molecular weight :

7 kDa

gene symbol :

SPINK1

details of functionality :

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002 ). The IC50 value is 10 nM, as measured under the described conditions.

endotoxin note :

<1.0 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

P00995

applications :

Enzyme Activity

source long :

E. coli -derived human SPINK1 protein Asp24-Cys79, with an N-terminal Met and 6-His tag

source short :

E. coli

USD :

541 USD

alt names :

pancreatic secretory trypsin inhibitor, PCTT, PCTTSpink3, PSTI, PSTISerine protease inhibitor Kazal-type 1, serine peptidase inhibitor, Kazal type 1, serine protease inhibitor, Kazal type 1, Spink3, TATI, TATITumor-associated trypsin inhibitor

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 ░C as supplied. 3 months, -70 ░C under sterile conditions after opening.