Recombinant Human Sirtuin 3/SIRT3 Protein, CF | R&D Systems 7488-DA-050 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Sirtuin 3/SIRT3 Protein, CF

catalog :

7488-DA-050

quantity :

50 ug

price :

432 USD

more info or order :

R&D Systems product webpage

citations: 1

Reference
James A, Hoogewijs K, Logan A, Hall A, Ding S, Fearnley I, et al. Non-enzymatic N-acetylation of Lysine Residues by AcetylCoA Often Occurs via a Proximal S-acetylated Thiol Intermediate Sensitive to Glyoxalase II. Cell Rep. 2017;18:2105-2112 pubmed publisher

product information

master code :

7488-DA

SKU :

7488-DA-050

product name :

Recombinant Human Sirtuin 3/SIRT3 Protein, CF

unit size :

50 ug

description :

The Recombinant Human Sirtuin 3/SIRT3 Protein, CF from R&D Systems is derived from E. coli. The Recombinant Human Sirtuin 3/SIRT3 Protein, CF has been validated for the following applications: Enzyme Activity.

target :

Sirtuin 3/SIRT3

category :

Proteins and Enzymes

buffer :

Supplied as a 0.2 ╡m filtered solution in Tris, NaCl, Brij-35 and Glycerol.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Human

observed molecular weight :

30-33 kDa, reducing conditions

theoretical molecular weight :

34 kDa

gene symbol :

SIRT3

details of functionality :

Measured by its ability to remove the acetyl group from a fluorogenic peptide substrate Ac-RGK(Ac)-AMC (Catalog # ES016 ) in a coupled assay. The specific activity is 2 pmol/min/╡g, as measured under the described conditions.

endotoxin note :

<1.0 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

Q9NTG7

applications :

Enzyme Activity

source long :

E. coli -derived human Sirtuin 3/SIRT3 protein Ser101-Lys399, with an N-terminal Met and 6-His tag

source short :

E. coli

USD :

432 USD

alt names :

EC 3.5.1, EC 3.5.1.-, hSIRT3, mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase, NAD-dependent deacetylase sirtuin-3, mitochondrial, SIR2L3, SIR2L3silent mating type information regulation 2, S.cerevisiae, homolog 3, sir2-like 3, SIR2-like protein 3, SIRT3, sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae), sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 3, sirtuin 3, sirtuin type 3

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 ░C as supplied. 3 months, -20 to -70 ░C under sterile conditions after opening.

more info or order :

R&D Systems product webpage