Recombinant Human Lefty-A Protein | R&D Systems 746-LF-025 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Lefty-A Protein

catalog :

746-LF-025

quantity :

25 ug

price :

432 USD

citations: 11

Reference
Zeng N, Okumura T, Alauddin M, Khozooei S, Rajaxavier J, Zhang S, et al. LEFTY2/endometrial bleeding-associated factor up-regulates Na+ Coupled Glucose Transporter SGLT1 expression and Glycogen Accumulation in Endometrial Cancer Cells. PLoS ONE. 2020;15:e0230044 pubmed publisher
Sprouse M, Welte T, Boral D, Liu H, Yin W, Vishnoi M, et al. PMN-MDSCs Enhance CTC Metastatic Properties through Reciprocal Interactions via ROS/Notch/Nodal Signaling. Int J Mol Sci. 2019;20: pubmed publisher
Jørgensen A, Macdonald J, Nielsen J, Kilcoyne K, Perlman S, Lundvall L, et al. Nodal Signaling Regulates Germ Cell Development and Establishment of Seminiferous Cords in the Human Fetal Testis. Cell Rep. 2018;25:1924-1937.e4 pubmed publisher
Chang K, Hertz J, Zhang X, Jin X, Shaw P, Derosa B, et al. Novel Regulatory Mechanisms for the SoxC Transcriptional Network Required for Visual Pathway Development. J Neurosci. 2017;37:4967-4981 pubmed publisher
Salker M, Hosseinzadeh Z, Alowayed N, Zeng N, Umbach A, Webster Z, et al. LEFTYA Activates the Epithelial Na+ Channel (ENaC) in Endometrial Cells via Serum and Glucocorticoid Inducible Kinase SGK1. Cell Physiol Biochem. 2016;39:1295-306 pubmed publisher
Salker M, Schierbaum N, Alowayed N, Singh Y, Mack A, Stournaras C, et al. LeftyA decreases Actin Polymerization and Stiffness in Human Endometrial Cancer Cells. Sci Rep. 2016;6:29370 pubmed publisher
Wilson T, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, et al. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature. 2012;487:505-9 pubmed publisher
Cavallari C, Fonsato V, Herrera M, Bruno S, Tetta C, Camussi G. Role of Lefty in the anti tumor activity of human adult liver stem cells. Oncogene. 2013;32:819-26 pubmed publisher
Salker M, Christian M, Steel J, Nautiyal J, Lavery S, Trew G, et al. Deregulation of the serum- and glucocorticoid-inducible kinase SGK1 in the endometrium causes reproductive failure. Nat Med. 2011;17:1509-13 pubmed publisher
McLean A, D Amour K, Jones K, Krishnamoorthy M, Kulik M, Reynolds D, et al. Activin a efficiently specifies definitive endoderm from human embryonic stem cells only when phosphatidylinositol 3-kinase signaling is suppressed. Stem Cells. 2007;25:29-38 pubmed
Maynadie M, Picard F, Husson B, Chatelain B, Cornet Y, Le Roux G, et al. Immunophenotypic clustering of myelodysplastic syndromes. Blood. 2002;100:2349-56 pubmed

product information

master code :

746-LF

SKU :

746-LF-025

product name :

Recombinant Human Lefty-A Protein

unit size :

25 ug

description :

The Recombinant Human Lefty-A Protein from R&D Systems is derived from NS0. The Recombinant Human Lefty-A Protein has been validated for the following applications: Bioactivity.

target :

Lefty-A

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Human

observed molecular weight :

44 kDa, 38 kDa and 34 kDa, reducing conditions

theoretical molecular weight :

39 kDa

gene symbol :

LEFTY2

details of functionality :

Measured by its ability to induce cell death using Mv1Lu mink lung epithelial cells. The ED50 for this effect is 0.5-3 ╡g/mL.

endotoxin note :

<1.0 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

Q64280

applications :

Bioactivity

source long :

Mouse myeloma cell line, NS0-derived human Lefty-A protein Mouse Lefty-1 Propeptide (Leu22-Arg77) Accession # Q64280 HHHHHH Human Lefty-A (Phe78-Pro366) Accession # O00292 N-terminus C-terminus

source short :

NS0

USD :

432 USD

alt names :

EBAFTGF-beta-4, endometrial bleeding associated factor, Endometrial bleeding-associated factor, Lefta, LEFTAProtein lefty-A, left-right determination factor 2, Left-right determination factor A, LEFTY2, LeftyA, LEFTYATransforming growth factor beta-4, MGC46222, Protein lefty-2, TGFB4endometrial bleeding associated factor (left-right determination, factor A;transforming growth factor beta superfamily)

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.