Recombinant Human t-Plasminogen Activator/tPA Protein, CF | R&D Systems 7449-SE-010 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human t-Plasminogen Activator/tPA Protein, CF

catalog :

7449-SE-010

quantity :

10 ug

price :

432 USD

more info or order :

R&D Systems product webpage

citations: 1

Reference
Yao L, Zhou Y, Li J, Wickens L, Conforti F, Rattu A, et al. Bidirectional epithelial-mesenchymal crosstalk provides self-sustaining profibrotic signals in pulmonary fibrosis. J Biol Chem. 2021;297:101096 pubmed publisher

product information

master code :

7449-SE

SKU :

7449-SE-010

product name :

Recombinant Human t-Plasminogen Activator/tPA Protein, CF

unit size :

10 ug

description :

The Recombinant Human t-Plasminogen Activator/tPA Protein, CF from R&D Systems is derived from CHO. The Recombinant Human t-Plasminogen Activator/tPA Protein, CF has been validated for the following applications: Enzyme Activity.

target :

t-Plasminogen Activator/tPA

category :

Proteins and Enzymes

buffer :

Supplied as a 0.2 ╡m filtered solution in MES, NaCl and CaCl2.

conjugate :

Unconjugated

purity :

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Human

observed molecular weight :

30-40 kDa, reducing conditions

theoretical molecular weight :

31 kDa (Chain A), 29 kDa (Chain B)

gene symbol :

PLAT

details of functionality :

Measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The specific activity is 190 pmol/min/╡g, as measured under the described conditions.

endotoxin note :

<1.0 EU per 1 ╡g of the protein by the LAL method.

top caption :

Recombinant Human t-Plasminogen Activator/tPA Protein Enzyme Activity

accessionNumbers :

P00750

applications :

Enzyme Activity

source long :

Chinese Hamster Ovary cell line, CHO-derived human t-Plasminogen Activator/tPA protein Met1-Pro562, with a C-terminal 6-His tag Single-chain proform was expressed, purified, activated with thermolysin to a 2-chain protease and further purified.

source short :

CHO

USD :

432 USD

alt names :

Alteplase, DKFZp686I03148, EC 3.4.21, EC 3.4.21.68, plasminogen activator, tissue, plasminogen activator, tissue type, PLAT, reteplase, tissue plasminogen activator (t-PA), tissue-type plasminogen activator, TPA, T-PA, t-plasminogen activator, tPlasminogen Activator

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 ░C as supplied. 3 months, -20 to -70 ░C under sterile conditions after opening.

more info or order :

R&D Systems product webpage