Recombinant Human Glucosylceramidase/GBA Protein, CF | R&D Systems 7410-GHB-020 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Glucosylceramidase/GBA Protein, CF

catalog :

7410-GHB-020

quantity :

20 ug (also 1 mg)

price :

514 USD

more info or order :

R&D Systems product webpage

citations: 11

Reference
Takahashi H, Bhagwagar S, Nies S, Ye H, Han X, Chiasseu M, et al. Reduced progranulin increases tau and α-synuclein inclusions and alters mouse tauopathy phenotypes via glucocerebrosidase. Nat Commun. 2024;15:1434 pubmed publisher
Russo T, Kolisnyk B, Bs A, Kim T, Martin J, Plessis Belair J, et al. The SATB1-MIR22-GBA axis mediates glucocerebroside accumulation inducing a cellular senescence-like phenotype in dopaminergic neurons. bioRxiv. 2023;: pubmed publisher
Brown A, Zhang J, Lawler B, Lu B. Recapture Lysosomal Enzyme Deficiency via Targeted Gene Disruption in the Human Near-Haploid Cell Line HAP1. Genes (Basel). 2021;12: pubmed publisher
Babcock M, Mikulka C, Wang B, Chandriani S, Chandra S, Xu Y, et al. Substrate reduction therapy for Krabbe disease and metachromatic leukodystrophy using a novel ceramide galactosyltransferase inhibitor. Sci Rep. 2021;11:14486 pubmed publisher
Oftedal L, Maple Grødem J, Førland M, Alves G, Lange J. Validation and assessment of preanalytical factors of a fluorometric in vitro assay for glucocerebrosidase activity in human cerebrospinal fluid. Sci Rep. 2020;10:22098 pubmed publisher
Do M, Levy D, Brown A, Marriott G, Lu B. Targeted delivery of lysosomal enzymes to the endocytic compartment in human cells using engineered extracellular vesicles. Sci Rep. 2019;9:17274 pubmed publisher
Li H, Ham A, Ma T, Kuo S, Kanter E, Kim D, et al. Mitochondrial dysfunction and mitophagy defect triggered by heterozygous GBA mutations. Autophagy. 2019;15:113-130 pubmed publisher
Yun S, Kim D, Kim S, Kim S, Karuppagounder S, Kwon S, et al. α-Synuclein accumulation and GBA deficiency due to L444P GBA mutation contributes to MPTP-induced parkinsonism. Mol Neurodegener. 2018;13:1 pubmed publisher
Ashmus R, Shen D, Vocadlo D. Fluorescence-Quenched Substrates for Quantitative Live Cell Imaging of Glucocerebrosidase Activity. Methods Enzymol. 2018;598:199-215 pubmed publisher
Choi S, Kim D, Kam T, Yun S, Kim S, Park H, et al. Lysosomal Enzyme Glucocerebrosidase Protects against AÎ²1-42 Oligomer-Induced Neurotoxicity. PLoS ONE. 2015;10:e0143854 pubmed publisher
Berger Z, Perkins S, Ambroise C, Oborski C, Calabrese M, Noell S, et al. Tool compounds robustly increase turnover of an artificial substrate by glucocerebrosidase in human brain lysates. PLoS ONE. 2015;10:e0119141 pubmed publisher

product information

brand :

R&D Systems, a Bio-Techne brand

catalog number base :

7410-GHB

SKU :

7410-GHB-020

product name :

Recombinant Human Glucosylceramidase/GBA Protein, CF

description :

The Recombinant Human Glucosylceramidase/GBA Protein, CF from R&D Systems is derived from CHO. The Recombinant Human Glucosylceramidase/GBA Protein, CF has been validated for the following applications: Enzyme Activity.

target :

Glucosylceramidase/GBA

unit size :

20 ug (also 1 mg)

category :

Proteins and Enzymes

buffer :

Supplied as a 0.2 µm filtered solution in Sodium Citrate.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

species :

Human

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

observed molecular weight :

60-75 kDa, reducing conditions

theoretical molecular weight :

56 kDa

details of functionality :

Measured by its ability to hydrolyze 4-methylumbelliferyl¿ß-D-glucopyranoside. The specific activity is 200 pmol/min/µg, as measured under the described conditions.

endotoxin note :

<1.0 EU per 1 µg of the protein by the LAL method.

accessionNumbers :

P04062.3

applications :

Enzyme Activity

product keywords :

7410-GH, rhGBA, hGBA, CF, carrier-free, carrier free, 741O-GHB, 7410GHB, bioprocessingenzymes, glycobiologyenzymes, Glycosidase, Glycosidases, GBA, GBA 1, Lysosomal Glucocerebrosidase, Acid Beta-Glucosidase, GLUC, D-Glucosyl-N-Acylsphingosine Glucohydrolase, Beta-Glucocerebrosidase, Gaucher disease, Lysosome Markers

source :

CHO-derived Recombinant Human Glucosylceramidase/GBA Protein, CF

USD :

499

USD 2025 :

514 USD

alt names :

Acid beta-glucosidase, alglucerase, Beta-glucocerebrosidase, D-glucosyl-N-acylsphingosine glucohydrolase, EC 3.2.1.45, GBA, GBA1, GC, GCB, GLUC, glucosidase, beta, acid, glucosidase, beta; acid (includes glucosylceramidase), glucosylceramidase, imiglucerase, lysosomal glucocerebrosidase

more info or order :

R&D Systems product webpage