This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
R&D Systems
product type :
protein
product name :
Recombinant Human Glucosylceramidase/GBA Protein, CF
catalog :
7410-GHB-020
quantity :
20 ug (also 1 mg)
price :
514 USD
citations: 12
Reference
Takahashi H, Bhagwagar S, Nies S, Ye H, Han X, Chiasseu M, et al. Reduced progranulin increases tau and α-synuclein inclusions and alters mouse tauopathy phenotypes via glucocerebrosidase. Nat Commun. 2024;15:1434 pubmed publisher
Russo T, Kolisnyk B, B S A, Plessis Belair J, Kim T, Martin J, et al. The SATB1-MIR22-GBA axis mediates glucocerebroside accumulation inducing a cellular senescence-like phenotype in dopaminergic neurons. Aging Cell. 2024;23:e14077 pubmed publisher
Russo T, Kolisnyk B, Bs A, Kim T, Martin J, Plessis Belair J, et al. The SATB1-MIR22-GBA axis mediates glucocerebroside accumulation inducing a cellular senescence-like phenotype in dopaminergic neurons. bioRxiv. 2023;: pubmed publisher
Brown A, Zhang J, Lawler B, Lu B. Recapture Lysosomal Enzyme Deficiency via Targeted Gene Disruption in the Human Near-Haploid Cell Line HAP1. Genes (Basel). 2021;12: pubmed publisher
Babcock M, Mikulka C, Wang B, Chandriani S, Chandra S, Xu Y, et al. Substrate reduction therapy for Krabbe disease and metachromatic leukodystrophy using a novel ceramide galactosyltransferase inhibitor. Sci Rep. 2021;11:14486 pubmed publisher
Oftedal L, Maple Grødem J, Førland M, Alves G, Lange J. Validation and assessment of preanalytical factors of a fluorometric in vitro assay for glucocerebrosidase activity in human cerebrospinal fluid. Sci Rep. 2020;10:22098 pubmed publisher
Do M, Levy D, Brown A, Marriott G, Lu B. Targeted delivery of lysosomal enzymes to the endocytic compartment in human cells using engineered extracellular vesicles. Sci Rep. 2019;9:17274 pubmed publisher
Li H, Ham A, Ma T, Kuo S, Kanter E, Kim D, et al. Mitochondrial dysfunction and mitophagy defect triggered by heterozygous GBA mutations. Autophagy. 2019;15:113-130 pubmed publisher
Yun S, Kim D, Kim S, Kim S, Karuppagounder S, Kwon S, et al. α-Synuclein accumulation and GBA deficiency due to L444P GBA mutation contributes to MPTP-induced parkinsonism. Mol Neurodegener. 2018;13:1 pubmed publisher
Ashmus R, Shen D, Vocadlo D. Fluorescence-Quenched Substrates for Quantitative Live Cell Imaging of Glucocerebrosidase Activity. Methods Enzymol. 2018;598:199-215 pubmed publisher
Choi S, Kim D, Kam T, Yun S, Kim S, Park H, et al. Lysosomal Enzyme Glucocerebrosidase Protects against Aβ1-42 Oligomer-Induced Neurotoxicity. PLoS ONE. 2015;10:e0143854 pubmed publisher
Berger Z, Perkins S, Ambroise C, Oborski C, Calabrese M, Noell S, et al. Tool compounds robustly increase turnover of an artificial substrate by glucocerebrosidase in human brain lysates. PLoS ONE. 2015;10:e0119141 pubmed publisher
product information
master code :
7410-GHB
SKU :
7410-GHB-020
product name :
Recombinant Human Glucosylceramidase/GBA Protein, CF
unit size :
20 ug (also 1 mg)
description :
The Recombinant Human Glucosylceramidase/GBA Protein, CF from R&D Systems is derived from CHO. The Recombinant Human Glucosylceramidase/GBA Protein, CF has been validated for the following applications: Enzyme Activity.
target :
Glucosylceramidase/GBA
category :
Proteins and Enzymes
buffer :
Supplied as a 0.2 ╡m filtered solution in Sodium Citrate.
conjugate :
Unconjugated
purity :
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.
species :
Human
observed molecular weight :
60-75 kDa, reducing conditions
theoretical molecular weight :
56 kDa
details of functionality :
Measured by its ability to hydrolyze 4-methylumbelliferyl┐▀-D-glucopyranoside. The specific activity is 200 pmol/min/╡g, as measured under the described conditions.
endotoxin note :
<1.0 EU per 1 ╡g of the protein by the LAL method.
accessionNumbers :
P04062.3
applications :
Enzyme Activity
source long :
Chinese Hamster Ovary cell line, CHO-derived human Glucosylceramidase/GBA protein Ala40-Gln536, with a C-terminal 6-His tag
source short :
CHO
USD :
514 USD
alt names :
Acid beta-glucosidase, alglucerase, Beta-glucocerebrosidase, D-glucosyl-N-acylsphingosine glucohydrolase, EC 3.2.1.45, GBA, GBA1, GC, GCB, GLUC, glucosidase, beta, acid, glucosidase, beta; acid (includes glucosylceramidase), glucosylceramidase, imiglucerase, lysosomal glucocerebrosidase
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 ░C as supplied. 3 months, -20 to -70 ░C under sterile conditions after opening.
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.