product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant GFP/SNAP25B/VAMP-2 Protein, CF
catalog :
7375-SV-050
quantity :
50 ug
price :
502 USD
more info or order :
R&D Systems product webpage
citations: 1
Reference
Albarran E, Sun Y, Liu Y, Raju K, Dong A, Li Y, et al. Postsynaptic synucleins mediate endocannabinoid signaling. Nat Neurosci. 2023;26:997-1007 pubmed publisher
product information
master code :
7375-SV
SKU :
7375-SV-050
product name :
Recombinant GFP/SNAP25B/VAMP-2 Protein, CF
unit size :
50 ug
description :
The Recombinant GFP/SNAP25B/VAMP-2 Protein, CF from R&D Systems is derived from E. coli. The Recombinant GFP/SNAP25B/VAMP-2 Protein, CF has been validated for the following applications: Enzyme Activity.
target :
GFP/SNAP25B/VAMP-2
category :
Proteins and Enzymes
buffer :
Supplied as a 0.2 ╡m filtered solution in Tris, NaCl, DTT and Tween«.
conjugate :
Unconjugated
purity :
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.
observed molecular weight :
54-58 kDa, reducing conditions
theoretical molecular weight :
52 kDa
details of functionality :
Measured by cleavage by botulinum and tetanus toxin light chains. 50% of 1 ╡g can be cleaved by 4 ng of toxin light chain, as measured under the described conditions.
endotoxin note :
<1.0 EU per 1 ╡g of the protein by the LAL method.
accessionNumbers :
ACS44347 (GFP); P60880 (SNAP25B); P63027 (VAMP-2)
applications :
Enzyme Activity
source long :
E. coli -derived GFP/SNAP25B/VAMP-2 protein MVKSADI 10-His tag Linker 1 GFP (Ser2-Lys238) Accession # ACS44347 Linker 2 Human SNAP25B (Asn93-Gly206) Accession # P60880 Human VAMP-2 (Ser2-Lys94) Accession # P63027 Cys N-terminus C-terminus
source short :
E. coli
extended description :
Substrate for Botulinum and Tetanus Neurotoxin Proteases
USD :
502 USD
product details :
Substrate for Botulinum and Tetanus Neurotoxin Proteases
alt names :
eGFP, GFP, GFPuv, Green Fluorescent Protein, RIC4, SEC9, SUP, SYB2, VAMP2
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 ░C as supplied. 3 months, -70 ░C under sterile conditions after opening.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.