Recombinant Human Wnt-5b Protein | R&D Systems 7347-WN product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Wnt-5b Protein

catalog :

7347-WN

quantity :

25 ug

price :

469 USD

citations: 9

Reference
de Rezende M, Ng Blichfeldt J, Justo G, Paredes Gamero E, Gosens R. Divergent effects of Wnt5b on IL-3- and GM-CSF-induced myeloid differentiation. Cell Signal. 2020;67:109507 pubmed publisher
Wu X, Van Dijk E, Ng Blichfeldt J, Bos I, Ciminieri C, Konigshoff M, et al. Mesenchymal WNT-5A/5B Signaling Represses Lung Alveolar Epithelial Progenitors. Cells. 2019;8: pubmed publisher
Wang S, Chang J, Hsiao J, Yen Y, Jiang S, Liu S, et al. Tumour cell-derived WNT5B modulates in vitro lymphangiogenesis via induction of partial endothelial-mesenchymal transition of lymphatic endothelial cells. Oncogene. 2017;36:1503-1515 pubmed publisher
Angbohang A, Wu N, Charalambous T, Eastlake K, Lei Y, Kim Y, et al. Downregulation of the Canonical WNT Signaling Pathway by TGFÎ²1 Inhibits Photoreceptor Differentiation of Adult Human MÃ¼ller Glia with Stem Cell Characteristics. Stem Cells Dev. 2016;25:1-12 pubmed publisher
Choudhary P, Dodsworth B, Sidders B, Gutteridge A, Michaelides C, Duckworth J, et al. A FOXM1 Dependent Mesenchymal-Epithelial Transition in Retinal Pigment Epithelium Cells. PLoS ONE. 2015;10:e0130379 pubmed publisher
Nicenboim J, Malkinson G, Lupo T, Asaf L, Sela Y, Mayseless O, et al. Lymphatic vessels arise from specialized angioblasts within a venous niche. Nature. 2015;522:56-61 pubmed
Kilander M, Dahlström J, Schulte G. Assessment of Frizzled 6 membrane mobility by FRAP supports G protein coupling and reveals WNT-Frizzled selectivity. Cell Signal. 2014;26:1943-9 pubmed publisher
Keats E, Dominguez J, Grant M, Khan Z. Switch from canonical to noncanonical Wnt signaling mediates high glucose-induced adipogenesis. Stem Cells. 2014;32:1649-60 pubmed publisher
Takeshita A, Iwai S, Morita Y, Niki Yonekawa A, Hamada M, Yura Y. Wnt5b promotes the cell motility essential for metastasis of oral squamous cell carcinoma through active Cdc42 and RhoA. Int J Oncol. 2014;44:59-68 pubmed publisher

product information

brand :

R&D Systems

master code :

7347-WN

SKU :

7347-WN-025

product name :

Recombinant Human Wnt-5b Protein

description :

The Recombinant Human Wnt-5b Protein from R&D Systems is derived from CHO. The Recombinant Human Wnt-5b Protein has been validated for the following applications: Bioactivity.

target :

Wnt-5b

category :

Proteins and Enzymes

unit size :

25 ug

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

species :

Human

observed molecular weight :

45 kDa, reducing conditions

theoretical molecular weight :

38.5 kDa

gene symbol :

WNT5B

details of functionality :

Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 125-750 ng/mL, in the presence of 5 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN ). Optimal dilutions should be determined by each laboratory for each application.

endotoxin note :

<0.10 EU per 1 µg of the protein by the LAL method.

catalog number base :

7347-WN

accessionNumbers :

NP_110402

applications :

Bioactivity

source :

Chinese Hamster Ovary cell line, CHO-derived human Wnt-5b protein Gln18-Lys359

2020 USD :

439

2021 USD :

469 USD

alt names :

MGC2648, protein Wnt-5b, wingless-type MMTV integration site family, member 5B, Wnt5b, WNT-5B protein

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.