product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human Wnt-5b Protein, CF
catalog :
7347-WN-025/CF
quantity :
25 ug
price :
578 USD
more info or order :
R&D Systems product webpage
citations: 13
Reference
Gupta S, Kawaguchi R, Heinrichs E, Gallardo S, Castellanos S, Mandric I, et al. In vitro atlas of dorsal spinal interneurons reveals Wnt signaling as a critical regulator of progenitor expansion. Cell Rep. 2022;40:111119 pubmed publisher
Wu C, Dicks A, Steward N, Tang R, Katz D, Choi Y, et al. Single cell transcriptomic analysis of human pluripotent stem cell chondrogenesis. Nat Commun. 2021;12:362 pubmed publisher
Rodriguez Hernandez I, Maiques O, Kohlhammer L, Cantelli G, Perdrix Rosell A, Monger J, et al. WNT11-FZD7-DAAM1 signalling supports tumour initiating abilities and melanoma amoeboid invasion. Nat Commun. 2020;11:5315 pubmed publisher
Nagel S, Pommerenke C, Meyer C, MacLeod R, Drexler H. Aberrant expression of NKL homeobox genes HMX2 and HMX3 interferes with cell differentiation in acute myeloid leukemia. PLoS ONE. 2020;15:e0240120 pubmed publisher
de Rezende M, Ng Blichfeldt J, Justo G, Paredes Gamero E, Gosens R. Divergent effects of Wnt5b on IL-3- and GM-CSF-induced myeloid differentiation. Cell Signal. 2020;67:109507 pubmed publisher
Wu X, Van Dijk E, Ng Blichfeldt J, Bos I, Ciminieri C, Konigshoff M, et al. Mesenchymal WNT-5A/5B Signaling Represses Lung Alveolar Epithelial Progenitors. Cells. 2019;8: pubmed publisher
Wang S, Chang J, Hsiao J, Yen Y, Jiang S, Liu S, et al. Tumour cell-derived WNT5B modulates in vitro lymphangiogenesis via induction of partial endothelial-mesenchymal transition of lymphatic endothelial cells. Oncogene. 2017;36:1503-1515 pubmed publisher
Angbohang A, Wu N, Charalambous T, Eastlake K, Lei Y, Kim Y, et al. Downregulation of the Canonical WNT Signaling Pathway by TGFβ1 Inhibits Photoreceptor Differentiation of Adult Human Müller Glia with Stem Cell Characteristics. Stem Cells Dev. 2016;25:1-12 pubmed publisher
Choudhary P, Dodsworth B, Sidders B, Gutteridge A, Michaelides C, Duckworth J, et al. A FOXM1 Dependent Mesenchymal-Epithelial Transition in Retinal Pigment Epithelium Cells. PLoS ONE. 2015;10:e0130379 pubmed publisher
Nicenboim J, Malkinson G, Lupo T, Asaf L, Sela Y, Mayseless O, et al. Lymphatic vessels arise from specialized angioblasts within a venous niche. Nature. 2015;522:56-61 pubmed
Kilander M, Dahlström J, Schulte G. Assessment of Frizzled 6 membrane mobility by FRAP supports G protein coupling and reveals WNT-Frizzled selectivity. Cell Signal. 2014;26:1943-9 pubmed publisher
Keats E, Dominguez J, Grant M, Khan Z. Switch from canonical to noncanonical Wnt signaling mediates high glucose-induced adipogenesis. Stem Cells. 2014;32:1649-60 pubmed publisher
Takeshita A, Iwai S, Morita Y, Niki Yonekawa A, Hamada M, Yura Y. Wnt5b promotes the cell motility essential for metastasis of oral squamous cell carcinoma through active Cdc42 and RhoA. Int J Oncol. 2014;44:59-68 pubmed publisher
product information
brand :
R&D Systems
master code :
7347-WN/CF
SKU :
7347-WN-025/CF
product name :
Recombinant Human Wnt-5b Protein, CF
description :
The Recombinant Human Wnt-5b Protein, CF from R&D Systems is derived from CHO. The Recombinant Human Wnt-5b Protein, CF has been validated for the following applications: Bioactivity.
target :
Wnt-5b
category :
Proteins and Enzymes
unit sizes :
25 ug
buffer :
Lyophilized from a 0.2 µm filtered solution in PBS, EDTA and CHAPS.
conjugate :
Unconjugated
purity :
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
species :
Human
observed molecular weight :
45 kDa, reducing conditions
theoretical molecular weight :
38.5 kDa
gene symbol :
WNT5B
details of functionality :
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 125-750 ng/mL, in the presence of 5 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN ). Optimal dilutions should be determined by each laboratory for each application.
endotoxin note :
<0.10 EU per 1 µg of the protein by the LAL method.
accessionNumbers :
NP_110402
applications :
Bioactivity
source :
CHO-derived Recombinant Human Wnt-5b Protein, CF
USD :
578 USD
alt names :
MGC2648, protein Wnt-5b, wingless-type MMTV integration site family, member 5B, Wnt5b, WNT-5B protein
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.