Recombinant Human Galactosylceramidase/GALC Protein, CF | R&D Systems 7310-GH-005 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Galactosylceramidase/GALC Protein, CF

catalog :

7310-GH-005

quantity :

5 ug

price :

567 USD

citations: 3

Reference
Babcock M, Mikulka C, Wang B, Chandriani S, Chandra S, Xu Y, et al. Substrate reduction therapy for Krabbe disease and metachromatic leukodystrophy using a novel ceramide galactosyltransferase inhibitor. Sci Rep. 2021;11:14486 pubmed publisher
Akiyama H, Ide M, Nagatsuka Y, Sayano T, Nakanishi E, Uemura N, et al. Glucocerebrosidases catalyze a transgalactosylation reaction that yields a newly-identified brain sterol metabolite, galactosylated cholesterol. J Biol Chem. 2020;295:5257-5277 pubmed publisher
Ullal A, Pham H, Singh R, Ross P, Graham C, Norton S, et al. Fluorimetric assay with a novel substrate for quantification of galactocerebrosidase activity in dried blood spot specimens. Pract Lab Med. 2020;18:e00141 pubmed publisher

product information

master code :

7310-GH

SKU :

7310-GH-005

product name :

Recombinant Human Galactosylceramidase/GALC Protein, CF

unit size :

5 ug

description :

The Recombinant Human Galactosylceramidase/GALC Protein, CF from R&D Systems is derived from CHO. The Recombinant Human Galactosylceramidase/GALC Protein, CF has been validated for the following applications: Enzyme Activity.

target :

Galactosylceramidase/GALC

category :

Proteins and Enzymes

buffer :

Supplied as a 0.2 ╡m filtered solution in MES, NaCl and Glycerol.

conjugate :

Unconjugated

purity :

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Human

observed molecular weight :

70-85 kDa, reducing conditions

theoretical molecular weight :

74 kDa

gene symbol :

GALC

details of functionality :

Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl┐▀-D-galactopyranoside. The specific activity is 550 pmol/min/ug, as measured under the described conditions.

endotoxin note :

<1.0 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

NP_000144

applications :

Enzyme Activity

source long :

Chinese Hamster Ovary cell line, CHO-derived human Galactosylceramidase/GALC protein Met1-Arg685, with a C-terminal 6-His tag

source short :

CHO

USD :

567 USD

alt names :

EC 3.2.1.46, galactocerebrosidase, Galactocerebroside beta-galactosidase, Galactosylceramidase, galactosylceramidase (Krabbe disease), Galactosylceramide beta-galactosidase, galactosylceraminidase, GALC, GALCERase

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 ░C as supplied. 3 months, -20 to -70 ░C under sterile conditions after opening.