Recombinant Human L-Selectin/CD62L Fc Chimera Protein, CF | R&D Systems 728-LS-100 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human L-Selectin/CD62L Fc Chimera Protein, CF

catalog :

728-LS-100

quantity :

100 ug

price :

394 USD

more info or order :

R&D Systems product webpage

citations: 12

Reference
Khan S, Xia Y, Goodale D, Schoettle G, Allan A. Lung-Derived Selectins Enhance Metastatic Behavior of Triple Negative Breast Cancer Cells. Biomedicines. 2021;9: pubmed publisher
Kelkar A, Zhu Y, Groth T, Stolfa G, Stablewski A, Singhi N, et al. Doxycycline-Dependent Self-Inactivation of CRISPR-Cas9 to Temporally Regulate On- and Off-Target Editing. Mol Ther. 2019;: pubmed publisher
Martin E, Malgor R, Resto V, Goetz D, Burdick M. Dynamic biochemical tissue analysis detects functional selectin ligands on human cancer tissues. Sci Rep. 2019;9:8511 pubmed publisher
Carlson G, Martin E, Shirure V, Malgor R, Resto V, Goetz D, et al. Dynamic biochemical tissue analysis detects functional L-selectin ligands on colon cancer tissues. PLoS ONE. 2017;12:e0173747 pubmed publisher
Li B, Juenet M, Aid Launais R, Maire M, Ollivier V, Letourneur D, et al. Development of Polymer Microcapsules Functionalized with Fucoidan to Target P-Selectin Overexpressed in Cardiovascular Diseases. Adv Healthc Mater. 2017;6: pubmed publisher
Robinson S, Thomas M, Simmons P, Lu J, Yang H, Javni J, et al. Non-fucosylated CB CD34+ cells represent a good target for enforced fucosylation to improve engraftment following cord blood transplantation. Cytotherapy. 2017;19:285-292 pubmed publisher
Pepin M, Mezouar S, Pegon J, Muczynski V, Adam F, Bianchini E, et al. Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity. Sci Rep. 2016;6:37953 pubmed publisher
Stolfa G, Mondal N, Zhu Y, Yu X, Buffone A, Neelamegham S. Using CRISPR-Cas9 to quantify the contributions of O-glycans, N-glycans and Glycosphingolipids to human leukocyte-endothelium adhesion. Sci Rep. 2016;6:30392 pubmed publisher
Krishnamurthy V, Sardar M, Ying Y, Song X, Haller C, Dai E, et al. Glycopeptide analogues of PSGL-1 inhibit P-selectin in vitro and in vivo. Nat Commun. 2015;6:6387 pubmed publisher
Riese S, Kuehne C, Tedder T, Hallmann R, Hohenester E, Buscher K. Heterotropic modulation of selectin affinity by allosteric antibodies affects leukocyte rolling. J Immunol. 2014;192:1862-9 pubmed publisher
Gakhar G, Navarro V, Jurish M, Lee G, Tagawa S, Akhtar N, et al. Circulating tumor cells from prostate cancer patients interact with E-selectin under physiologic blood flow. PLoS ONE. 2013;8:e85143 pubmed publisher
Prakobphol A, Genbacev O, Gormley M, Kapidzic M, Fisher S. A role for the L-selectin adhesion system in mediating cytotrophoblast emigration from the placenta. Dev Biol. 2006;298:107-17 pubmed

product information

brand :

R&D Systems

master code :

728-LS

SKU :

728-LS-100

product name :

Recombinant Human L-Selectin/CD62L Fc Chimera Protein, CF

description :

The Recombinant Human L-Selectin/CD62L Fc Chimera Protein, CF from R&D Systems is derived from NS0. The Recombinant Human L-Selectin/CD62L Fc Chimera Protein, CF has been validated for the following applications: Bioactivity.

target :

L-Selectin/CD62L

category :

Proteins and Enzymes

unit sizes :

100 ug

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS.

conjugate :

Unconjugated

purity :

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

species :

Human

observed molecular weight :

90-100 kDa, reducing conditions

theoretical molecular weight :

60.4 kDa (monomer)

gene symbol :

SELL

details of functionality :

Measured by the ability of the immobilized protein to support the adhesion of LS180 human colorectal adenocarcinoma cells. When 5 x 104 cells/well are added to Recombinant Human L-Selectin/CD62L Fc Chimera coated plates, adhesion is induced in a dose dependent manner after 1 hour incubation at 37 °C. The ED50 for this effect is 0.4-2 µg/mL. Optimal dilutions should be determined by each laboratory for each application.

endotoxin note :

<0.01 EU per 1 µg of the protein by the LAL method.

accessionNumbers :

P14151

applications :

Bioactivity

source :

NS0-derived Recombinant Human L-Selectin/CD62L Fc Chimera Protein, CF

USD :

394 USD

alt names :

CD62L, CD62L antigen, gp90-MEL, hLHRc, LAM1, LAM-1, LAM1LECAM1, LECAM1, Leu-8, LEU8, Leukocyte adhesion molecule 1, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, LNHR, LNHRTQ1, LSEL, L-selectin, Lyam-1, LYAM1, LYAM1CD62 antigen-like family member L, Lymph node homing receptor, lymphocyte adhesion molecule 1, pln homing receptor, PLNHR, selectin L, SELL, TQ1

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage