Recombinant Human Caspase-3 Protein | R&D Systems 707-C3 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Caspase-3 Protein

catalog :

707-C3

quantity :

10 ug

price :

379 USD

citations: 11

Reference
Lee Y, Shu M, Kim J, Kim Y, Sim K, Sung W, et al. Cilostazol protects hepatocytes against alcohol-induced apoptosis via activation of AMPK pathway. PLoS ONE. 2019;14:e0211415 pubmed publisher
Qin X, Lyu M, Si Y, Yang J, Wu Z, Li J. Alkyne-based surface-enhanced Raman scattering nanoprobe for ratiometric imaging analysis of caspase 3 in live cells and tissues. Anal Chim Acta. 2018;1043:115-122 pubmed publisher
Zhou B, Zhang J, Liu X, Chen H, Ai Y, Cheng K, et al. Tom20 senses iron-activated ROS signaling to promote melanoma cell pyroptosis. Cell Res. 2018;28:1171-1185 pubmed publisher
Wang F, Wang L, Wu J, Sokirniy I, Nguyen P, Bregnard T, et al. Active site-targeted covalent irreversible inhibitors of USP7 impair the functions of Foxp3+ T-regulatory cells by promoting ubiquitination of Tip60. PLoS ONE. 2017;12:e0189744 pubmed publisher
Shim M, Yoon H, Lee S, Jo M, Park J, Kim J, et al. Caspase-3/-7-Specific Metabolic Precursor for Bioorthogonal Tracking of Tumor Apoptosis. Sci Rep. 2017;7:16635 pubmed publisher
Taabazuing C, Okondo M, Bachovchin D. Pyroptosis and Apoptosis Pathways Engage in Bidirectional Crosstalk in Monocytes and Macrophages. Cell Chem Biol. 2017;24:507-514.e4 pubmed publisher
Pérez López A, Soria Gila M, Marsden E, Lilienkampf A, Bradley M. Fluorogenic Substrates for In Situ Monitoring of Caspase-3 Activity in Live Cells. PLoS ONE. 2016;11:e0153209 pubmed publisher
Wejda M, Impens F, Takahashi N, Van Damme P, Gevaert K, Vandenabeele P. Degradomics reveals that cleavage specificity profiles of caspase-2 and effector caspases are alike. J Biol Chem. 2012;287:33983-95 pubmed publisher
Guery L, Benikhlef N, Gautier T, Paul C, Jego G, Dufour E, et al. Fine-tuning nucleophosmin in macrophage differentiation and activation. Blood. 2011;118:4694-704 pubmed publisher
Ghosh J, Shenoy A, Clark J. Interactions between important regulatory proteins and human alphaB crystallin. Biochemistry. 2007;46:6308-17 pubmed
Lane D, Côté M, Grondin R, Couture M, Piche A. Acquired resistance to TRAIL-induced apoptosis in human ovarian cancer cells is conferred by increased turnover of mature caspase-3. Mol Cancer Ther. 2006;5:509-21 pubmed

product information

SKU :

707-C3-010

product name :

Recombinant Human Caspase-3 Protein

unit size :

10 ug

Description :

The Recombinant Human Caspase-3 Protein from R D Systems is derived from E. coli. The Recombinant Human Caspase-3 Protein has been validated for the following applications: Enzyme Activity.

target :

Caspase-3

category :

Proteins and Enzymes

buffer :

Supplied as a 0.2 µm filtered solution in HEPES, NaCl, DTT and Sucrose with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

species :

Human

observed molecular weight :

18 kDa and 10 kDa, reducing conditions

theoretical molecular weight :

17 kDa (subunit 1), 11 kDa (subunit 2)

gene symbol :

CASP3

details of functionality :

Measured by its ability to cleave the fluorogenic peptide substrate Ac-DEVD-AFC. The specific activity is 3,000 pmol/min/µg, as measured under the described conditions.

endotoxin note :

1.0 EU per 1 µg of the protein by the LAL method.

catalog number base :

707-C3

accessionNumbers :

P42574

applications :

Enzyme Activity

source :

E. coli-derived Recombinant Human Caspase-3 Protein

2020 Proposedprice USD :

379 USD

alt names :

Apopain, apoptosis-related cysteine protease, CASP3, CASP-3, caspase 3, apoptosis-related cysteine peptidase, caspase-3, Caspase3, CPP32, CPP-32, CPP32B, CPP32SREBP cleavage activity 1, Cysteine protease CPP32, EC 3.4.22, EC 3.4.22.56, LICE-1, PARP cleavage protease, procaspase3, Protein Yama, SCA-1, Yama

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.