Recombinant Human Caspase-3 Protein, CF | R&D Systems 707-C3-010/CF product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Caspase-3 Protein, CF

catalog :

707-C3-010/CF

quantity :

10 ug

price :

480 USD

citations: 16

Reference
Ai Y, Wang W, Liu F, Fang W, Chen H, Wu L, et al. Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P. Cell Res. 2023;: pubmed publisher
Bosc E, Anastasie J, Soualmia F, Coric P, Kim J, Wang L, et al. Genuine selective caspase-2 inhibition with new irreversible small peptidomimetics. Cell Death Dis. 2022;13:959 pubmed publisher
Zhang J, Zhou B, Sun R, Ai Y, Cheng K, Li F, et al. The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8. Cell Res. 2021;: pubmed publisher
Shen H, Zhu H, Panja D, Gu Q, Li Z. Autophagy controls the induction and developmental decline of NMDAR-LTD through endocytic recycling. Nat Commun. 2020;11:2979 pubmed publisher
Huo D, Zhu J, Chen G, Chen Q, Zhang C, Luo X, et al. Eradication of unresectable liver metastasis through induction of tumour specific energy depletion. Nat Commun. 2019;10:3051 pubmed publisher
Lee Y, Shu M, Kim J, Kim Y, Sim K, Sung W, et al. Cilostazol protects hepatocytes against alcohol-induced apoptosis via activation of AMPK pathway. PLoS ONE. 2019;14:e0211415 pubmed publisher
Qin X, Lyu M, Si Y, Yang J, Wu Z, Li J. Alkyne-based surface-enhanced Raman scattering nanoprobe for ratiometric imaging analysis of caspase 3 in live cells and tissues. Anal Chim Acta. 2018;1043:115-122 pubmed publisher
Zhou B, Zhang J, Liu X, Chen H, Ai Y, Cheng K, et al. Tom20 senses iron-activated ROS signaling to promote melanoma cell pyroptosis. Cell Res. 2018;28:1171-1185 pubmed publisher
Wang F, Wang L, Wu J, Sokirniy I, Nguyen P, Bregnard T, et al. Active site-targeted covalent irreversible inhibitors of USP7 impair the functions of Foxp3+ T-regulatory cells by promoting ubiquitination of Tip60. PLoS ONE. 2017;12:e0189744 pubmed publisher
Shim M, Yoon H, Lee S, Jo M, Park J, Kim J, et al. Caspase-3/-7-Specific Metabolic Precursor for Bioorthogonal Tracking of Tumor Apoptosis. Sci Rep. 2017;7:16635 pubmed publisher
Pérez López A, Soria Gila M, Marsden E, Lilienkampf A, Bradley M. Fluorogenic Substrates for In Situ Monitoring of Caspase-3 Activity in Live Cells. PLoS ONE. 2016;11:e0153209 pubmed publisher
Wejda M, Impens F, Takahashi N, Van Damme P, Gevaert K, Vandenabeele P. Degradomics reveals that cleavage specificity profiles of caspase-2 and effector caspases are alike. J Biol Chem. 2012;287:33983-95 pubmed publisher
Jarde T, Evans R, McQuillan K, Parry L, Feng G, Alvares B, et al. In vivo and in vitro models for the therapeutic targeting of Wnt signaling using a Tet-OΔN89β-catenin system. Oncogene. 2013;32:883-93 pubmed publisher
Guery L, Benikhlef N, Gautier T, Paul C, Jego G, Dufour E, et al. Fine-tuning nucleophosmin in macrophage differentiation and activation. Blood. 2011;118:4694-704 pubmed publisher
Ghosh J, Shenoy A, Clark J. Interactions between important regulatory proteins and human alphaB crystallin. Biochemistry. 2007;46:6308-17 pubmed
Lane D, Côté M, Grondin R, Couture M, Piche A. Acquired resistance to TRAIL-induced apoptosis in human ovarian cancer cells is conferred by increased turnover of mature caspase-3. Mol Cancer Ther. 2006;5:509-21 pubmed

product information

master code :

707-C3/CF

SKU :

707-C3-010/CF

product name :

Recombinant Human Caspase-3 Protein, CF

unit size :

10 ug

description :

The Recombinant Human Caspase-3 Protein, CF from R&D Systems is derived from E. coli. The Recombinant Human Caspase-3 Protein, CF has been validated for the following applications: Enzyme Activity.

target :

Caspase-3

category :

Proteins and Enzymes

buffer :

Supplied as a 0.2 ╡m filtered solution in HEPES, NaCl, DTT and Sucrose.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Human

observed molecular weight :

18 kDa and 10 kDa, reducing conditions

theoretical molecular weight :

17 kDa (subunit 1), 11 kDa (subunit 2)

gene symbol :

CASP3

details of functionality :

Measured by its ability to cleaveáthe fluorogenic peptide substrate Ac-DEVD-AFC. The specific activity is 3,000 pmol/min/╡g, as measured under the described conditions.

endotoxin note :

<1.0 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

P42574

applications :

Enzyme Activity

source long :

E. coli -derived human Caspase-3 protein Ser29-Asp175 (subunit 1) & Ala183-His277 (Asp190Glu) (subunit 2)

source short :

E. coli

USD :

480 USD

alt names :

Apopain, apoptosis-related cysteine protease, CASP3, CASP-3, caspase 3, apoptosis-related cysteine peptidase, caspase-3, Caspase3, CPP32, CPP-32, CPP32B, CPP32SREBP cleavage activity 1, Cysteine protease CPP32, EC 3.4.22, EC 3.4.22.56, LICE-1, PARP cleavage protease, procaspase3, Protein Yama, SCA-1, Yama

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 ░C as supplied. 3 months, -70 ░C under sterile conditions after opening.