Recombinant P. heparinus Chondroitinase B Protein, CF | R&D Systems 6974-GH-020 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant P. heparinus Chondroitinase B Protein, CF

catalog :

6974-GH-020

quantity :

20 ug

price :

456 USD

more info or order :

R&D Systems product webpage

citations: 4

Reference
Persson A, Vorontsov E, Larson G, Nilsson J. Glycosaminoglycan Domain Mapping of Cellular Chondroitin/Dermatan Sulfates. Sci Rep. 2020;10:3506 pubmed publisher
Persson A, Gomez Toledo A, Vorontsov E, Nasir W, Willen D, Noborn F, et al. LC-MS/MS characterization of xyloside-primed glycosaminoglycans with cytotoxic properties reveals structural diversity and novel glycan modifications. J Biol Chem. 2018;293:10202-10219 pubmed publisher
Persson A, Tykesson E, Westergren Thorsson G, Malmstrom A, Ellervik U, Mani K. Xyloside-primed Chondroitin Sulfate/Dermatan Sulfate from Breast Carcinoma Cells with a Defined Disaccharide Composition Has Cytotoxic Effects in Vitro. J Biol Chem. 2016;291:14871-82 pubmed publisher
Gouignard N, Maccarana M, Strate I, von Stedingk K, Malmstrom A, Pera E. Musculocontractural Ehlers-Danlos syndrome and neurocristopathies: dermatan sulfate is required for Xenopus neural crest cells to migrate and adhere to fibronectin. Dis Model Mech. 2016;9:607-20 pubmed publisher

product information

master code :

6974-GH

SKU :

6974-GH-020

product name :

Recombinant P. heparinus Chondroitinase B Protein, CF

unit size :

20 ug

description :

The Recombinant P. heparinus Chondroitinase B Protein, CF from R&D Systems is derived from E. coli. The Recombinant P. heparinus Chondroitinase B Protein, CF has been validated for the following applications: Enzyme Activity.

target :

Chondroitin B Lyase/Chondroitinase B

category :

Proteins and Enzymes

buffer :

Supplied as a 0.2 ╡m filtered solution in Tris and NaCl.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie« Blue stain at 5 ╡g per lane

species :

P. heparinus

observed molecular weight :

52-56 kDa, reducing conditions

theoretical molecular weight :

55 kDa

gene symbol :

Phep_0789

details of functionality :

Measured by its ability to hydrolyze chondroitin sulfate B. The specific activity is 90,000 pmol/min/╡g, as measured under the described conditions.

endotoxin note :

<1.0 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

Q46079

applications :

Enzyme Activity

source long :

E. coli -derived p. heparinus Chondroitin B Lyase/Chondroitinase B protein Gln26-His506, with an N-terminal Met and 6-His tag

source short :

E. coli

USD :

456 USD

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 ░C as supplied. 3 months, -20 to -70 ░C under sterile conditions after opening.

more info or order :

R&D Systems product webpage