mouse monoclonal (611621)
application: immunohistochemistry
citations: 1
application: immunohistochemistry
citations: 1
Immunohistochemistry Netrin-4 was detected in perfusion fixed frozen sections of mouse brain (nucleus accumbens) using Goat Anti-Mouse Netrin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1132 ) conjugated to Digoxigenin at 15 ╡g/mL overnight at 4 ░C. Tissue was stained using 25 ╡g/mL Mouse Anti-Digoxigenin Monoclonal Antibody (Catalog # MAB7520 ) followed by incubation with NorthernLightsÖ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007 ). Tissue was counter-stained with DAPI (blue). Specific staining was localized to vasculature. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections .
quantity: 100 ug (also 25 ug)
price: 349 USD
to the supplier
mouse monoclonal (611621)
conjugate: AF488
application: immunohistochemistry, immunocytochemistry
conjugate: AF488
application: immunohistochemistry, immunocytochemistry
Immunohistochemistry Netrin-4 was detected in perfusion fixed frozen sections of mouse brain (nucleus accumbens) using digoxigenin-conjugated Goat Anti-Mouse Netrin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1132 ) at 5 ╡g/mL overnight at 4 ░C. Tissue was stained using the Digoxigenin Alexa Fluor« 488-conjugated Monoclonal Antibody (green; Catalog # IC7520G ) at a final concentration of 1X (1:10 dilution) and counterstained with DAPI (blue). Specific staining was localized to vasculature. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections .
quantity: 100 Tests
price: 349 USD
to the supplier
mouse monoclonal (611532)
application: western blot
application: western blot
Detection of Digoxigenin by Western Blot. Western blot shows lysates of Goat IgG labeled with Digoxigenin. PVDF membrane was probed with 0.1 ╡g/mL of Mouse Anti-Digoxigenin Monoclonal Antibody (Catalog # MAB75201) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018 ). Specific bands were detected for IgG labeled with Digoxigenin at approximately 25 and 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .
quantity: 100 ug (also 25 ug)
price: 349 USD
to the supplier
mouse monoclonal (611621R)
application: immunohistochemistry
application: immunohistochemistry
Digoxigenin-Conjugated Antibody in Human Prostate Cancer Tissue. PKM2 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human/Mouse/Rat PKM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7244 ) conjugated to Digoxigenin at 10 ╡g/mL overnight at 4 ░C, followed by Mouse Anti-Digoxigenin Recombinant Monoclonal Antibody (Catalog # MAB7520R) at 5 ╡g/mL for 1 hour at room temperature. Tissue was stained using the Anti-Mouse HRP-DAB Cell Tissue Staining Kit (brown; Catalog # CTS002 ) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections .
quantity: 1 mg
price: 1399 USD
to the supplier