Recombinant Porcine TNF-alpha Protein | R&D Systems 690-PT-025 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Porcine TNF-alpha Protein

catalog :

690-PT-025

quantity :

25 ug

price :

493 USD

more info or order :

R&D Systems product webpage

citations: 9

Reference
Liniger M, Gerber M, Renzullo S, García Nicolás O, Ruggli N. TNF-Mediated Inhibition of Classical Swine Fever Virus Replication Is IRF1-, NF-κB- and JAK/STAT Signaling-Dependent. Viruses. 2021;13: pubmed publisher
Hohnstein F, Meurer M, de Buhr N, von Köckritz Blickwede M, Baums C, Alber G, et al. Analysis of Porcine Pro- and Anti-Inflammatory Cytokine Induction by S. suis In Vivo and In Vitro. Pathogens. 2020;9: pubmed publisher
Hall S, Ison S, Owles C, Coe J, Sandercock D, Zanella A. Development and validation of a multiplex fluorescent microsphere immunoassay assay for detection of porcine cytokines. MethodsX. 2019;6:1218-1227 pubmed publisher
Franzoni G, Graham S, Sanna G, Angioi P, Fiori M, Anfossi A, et al. Interaction of porcine monocyte-derived dendritic cells with African swine fever viruses of diverse virulence. Vet Microbiol. 2018;216:190-197 pubmed publisher
Singleton H, Graham S, Frossard J, Bodman Smith K, Steinbach F. Infection of monocytes with European porcine reproductive and respiratory syndrome virus (PRRSV-1) strain Lena is significantly enhanced by dexamethasone and IL-10. Virology. 2018;517:199-207 pubmed publisher
Clyne A, Zhu H, Edelman E. Elevated fibroblast growth factor-2 increases tumor necrosis factor-alpha induced endothelial cell death in high glucose. J Cell Physiol. 2008;217:86-92 pubmed publisher
McNulty A, Moutos F, Weinberg J, Guilak F. Enhanced integrative repair of the porcine meniscus in vitro by inhibition of interleukin-1 or tumor necrosis factor alpha. Arthritis Rheum. 2007;56:3033-42 pubmed
Johannisson A, Jonasson R, Dernfalk J, Jensen Waern M. Simultaneous detection of porcine proinflammatory cytokines using multiplex flow cytometry by the xMAP technology. Cytometry A. 2006;69:391-5 pubmed
Azevedo M, Yuan L, Pouly S, Gonzales A, Jeong K, Nguyen T, et al. Cytokine responses in gnotobiotic pigs after infection with virulent or attenuated human rotavirus. J Virol. 2006;80:372-82 pubmed

product information

brand :

R&D Systems, a Bio-Techne Brand

catalog number base :

690-PT

SKU :

690-PT-025

product name :

Recombinant Porcine TNF-alpha Protein

Description :

The Recombinant Porcine TNF-alpha Protein from R&D Systems is derived from E. coli. The Recombinant Porcine TNF-alpha Protein has been validated for the following applications: Bioactivity.

target :

TNF-alpha

category :

Proteins and Enzymes

unit size :

25 ug

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain

species :

Porcine

theoretical molecular weight :

17 kDa

gene symbol :

TNF

details of functionality :

Measured in a cytotoxicity assay using TNF-susceptible PK-15 porcine kidney epithelial cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al . (eds): IRL Press. 221; Bertoni et al . (1993) J. Immunol. Meth. 160 :267. The ED50 for this effect is 0.003-0.018 ng/mL.

endotoxin note :

<0.01 EU per 1 µg of the protein by the LAL method.

accessionNumbers :

P23563

applications :

Bioactivity

source :

E. coli-derived Recombinant Porcine TNF-alpha Protein

USD :

493 USD

alt names :

APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage