product summary
Loading...
company name :
R&D Systems
product type :
protein
product name :
Recombinant Porcine TNF-alpha Protein, CF
catalog :
690-PT-025/CF
quantity :
25 ug
price :
493 USD
more info or order :
R&D Systems product webpage
citations: 9
Reference
Liniger M, Gerber M, Renzullo S, García Nicolás O, Ruggli N. TNF-Mediated Inhibition of Classical Swine Fever Virus Replication Is IRF1-, NF-κB- and JAK/STAT Signaling-Dependent. Viruses. 2021;13: pubmed publisher
Hohnstein F, Meurer M, de Buhr N, von Köckritz Blickwede M, Baums C, Alber G, et al. Analysis of Porcine Pro- and Anti-Inflammatory Cytokine Induction by S. suis In Vivo and In Vitro. Pathogens. 2020;9: pubmed publisher
Hall S, Ison S, Owles C, Coe J, Sandercock D, Zanella A. Development and validation of a multiplex fluorescent microsphere immunoassay assay for detection of porcine cytokines. MethodsX. 2019;6:1218-1227 pubmed publisher
Franzoni G, Graham S, Sanna G, Angioi P, Fiori M, Anfossi A, et al. Interaction of porcine monocyte-derived dendritic cells with African swine fever viruses of diverse virulence. Vet Microbiol. 2018;216:190-197 pubmed publisher
Singleton H, Graham S, Frossard J, Bodman Smith K, Steinbach F. Infection of monocytes with European porcine reproductive and respiratory syndrome virus (PRRSV-1) strain Lena is significantly enhanced by dexamethasone and IL-10. Virology. 2018;517:199-207 pubmed publisher
Clyne A, Zhu H, Edelman E. Elevated fibroblast growth factor-2 increases tumor necrosis factor-alpha induced endothelial cell death in high glucose. J Cell Physiol. 2008;217:86-92 pubmed publisher
McNulty A, Moutos F, Weinberg J, Guilak F. Enhanced integrative repair of the porcine meniscus in vitro by inhibition of interleukin-1 or tumor necrosis factor alpha. Arthritis Rheum. 2007;56:3033-42 pubmed
Johannisson A, Jonasson R, Dernfalk J, Jensen Waern M. Simultaneous detection of porcine proinflammatory cytokines using multiplex flow cytometry by the xMAP technology. Cytometry A. 2006;69:391-5 pubmed
Azevedo M, Yuan L, Pouly S, Gonzales A, Jeong K, Nguyen T, et al. Cytokine responses in gnotobiotic pigs after infection with virulent or attenuated human rotavirus. J Virol. 2006;80:372-82 pubmed
product information
brand :
R&D Systems, a Bio-Techne Brand
catalog number base :
690-PT/CF
SKU :
690-PT-025/CF
product name :
Recombinant Porcine TNF-alpha Protein, CF
Description :
The Recombinant Porcine TNF-alpha Protein, CF from R&D Systems is derived from E. coli. The Recombinant Porcine TNF-alpha Protein, CF has been validated for the following applications: Bioactivity.
target :
TNF-alpha
category :
Proteins and Enzymes
unit size :
25 ug
buffer :
Lyophilized from a 0.2 µm filtered solution in PBS.
conjugate :
Unconjugated
purity :
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
species :
Porcine
theoretical molecular weight :
17 kDa
gene symbol :
TNF
details of functionality :
Measured in a cytotoxicity assay using TNF-susceptible PK-15 porcine kidney epithelial cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al . (eds): IRL Press. 221; Bertoni et al . (1993) J. Immunol. Meth. 160 :267. The ED50 for this effect is 0.003-0.018 ng/mL.
endotoxin note :
<0.01 EU per 1 µg of the protein by the LAL method.
accessionNumbers :
P23563
applications :
Bioactivity
source :
E. coli-derived Recombinant Porcine TNF-alpha Protein
USD :
493 USD
alt names :
APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.