Recombinant B. thetaiotaomicron O-GlcNAcase/OGA Protein, CF | R&D Systems 6779-GH-020 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant B. thetaiotaomicron O-GlcNAcase/OGA Protein, CF

catalog :

6779-GH-020

quantity :

20 ug

price :

456 USD

citations: 4

Reference
Tachida Y, Iijima J, Takahashi K, Suzuki H, Kizuka Y, Yamaguchi Y, et al. O-GalNAc glycosylation determines intracellular trafficking of APP and Aβ production. J Biol Chem. 2023;299:104905 pubmed publisher
Dodson M, Liu P, Jiang T, Ambrose A, Luo G, Rojo de la Vega M, et al. Increased O-GlcNAcylation of SNAP29 Drives Arsenic-Induced Autophagic Dysfunction. Mol Cell Biol. 2018;38: pubmed publisher
Wu Z, Person A, Anderson M, Burroughs B, Tatge T, Khatri K, et al. Imaging specific cellular glycan structures using glycosyltransferases via click chemistry. Glycobiology. 2017;: pubmed publisher
Rambaruth N, Greenwell P, Dwek M. The lectin Helix pomatia agglutinin recognizes O-GlcNAc containing glycoproteins in human breast cancer. Glycobiology. 2012;22:839-48 pubmed publisher

product information

master code :

6779-GH

SKU :

6779-GH-020

product name :

Recombinant B. thetaiotaomicron O-GlcNAcase/OGA Protein, CF

unit size :

20 ug

description :

The Recombinant B. thetaiotaomicron O-GlcNAcase/OGA Protein, CF from R&D Systems is derived from E. coli. The Recombinant B. thetaiotaomicron O-GlcNAcase/OGA Protein, CF has been validated for the following applications: Enzyme Activity.

target :

O-GlcNAcase/OGA

category :

Proteins and Enzymes

buffer :

Supplied as a 0.2 ╡m filtered solution in Tris, NaCl, Brij and Glycerol.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie« Blue stain at 5 ╡g per lane

species :

B. thetaiotaomicron

observed molecular weight :

66-76 kDa, reducing conditions

theoretical molecular weight :

83 kDa

gene symbol :

BT_4395

details of functionality :

Measured by its ability to hydrolyze 4-methylumbelliferyl-N-acetyl┐▀-D-glucosaminide (4-MU-GlcNAc) The specific activity is 3500 pmol/min/╡g, as measured under the described conditions.

endotoxin note :

<1.0 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

Q89ZI2

applications :

Enzyme Activity

source long :

E. coli -derived b. thetaiotaomicron O-GlcNAcase/OGA protein Gln22-Lys737, with an N-terminal Met and 6-His tag

source short :

E. coli

USD :

456 USD

alt names :

bifunctional protein NCOAT, FLJ11229, GH84, HEXC, HEXC3, Hexosaminidase B, hyaluronidase in meningioma, KIAA0679, MEA5FLJ23355, meningioma expressed antigen 5 (hyaluronidase), Meningioma-expressed antigen 5, MGEA5, NCOAT, Nuclear cytoplasmic O-GlcNAcase and acetyltransferase, OGA, OGlcNAcase, O-GlcNAcase

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 ░C as supplied. 3 months, -20 to -70 ░C under sterile conditions after opening.