Recombinant Human IGFBP-3 Protein, CF | R&D Systems 675-B3-025 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human IGFBP-3 Protein, CF

catalog :

675-B3-025

quantity :

25 ug

price :

407 USD

more info or order :

R&D Systems product webpage

citations: 12

Reference
Ng E, Kaida A, Nojima H, Miura M. Roles of IGFBP-3 in cell migration and growth in an endophytic tongue squamous cell carcinoma cell line. Sci Rep. 2022;12:11503 pubmed publisher
Shih H, Chang H, Chen C, Torng P. Differential expression of hypoxia-inducible factors related to the invasiveness of epithelial ovarian cancer. Sci Rep. 2021;11:22925 pubmed publisher
Wirthgen E, Goumon S, Kunze M, Walz C, Spitschak M, Tuchscherer A, et al. Effects of Transport Duration and Environmental Conditions in Winter or Summer on the Concentrations of Insulin-Like Growth Factors and Insulin-Like Growth Factor-Binding Proteins in the Plasma of Market-Weight Pigs. Front Endocrinol (Lausanne). 2018;9:36 pubmed publisher
Svalina M, Kikuchi K, Abraham J, Lal S, Davare M, Settelmeyer T, et al. IGF1R as a Key Target in High Risk, Metastatic Medulloblastoma. Sci Rep. 2016;6:27012 pubmed publisher
Elzi D, Lai Y, Song M, Hakala K, Weintraub S, Shiio Y. Plasminogen activator inhibitor 1--insulin-like growth factor binding protein 3 cascade regulates stress-induced senescence. Proc Natl Acad Sci U S A. 2012;109:12052-7 pubmed publisher
Bogatkevich G, Ludwicka Bradley A, Highland K, Hant F, Nietert P, Singleton C, et al. Impairment of the antifibrotic effect of hepatocyte growth factor in lung fibroblasts from African Americans: possible role in systemic sclerosis. Arthritis Rheum. 2007;56:2432-42 pubmed
Rosendahl A, Forsberg G. IGF-I and IGFBP-3 augment transforming growth factor-beta actions in human renal carcinoma cells. Kidney Int. 2006;70:1584-90 pubmed
Schnapp L, Donohoe S, Chen J, Sunde D, Kelly P, Ruzinski J, et al. Mining the acute respiratory distress syndrome proteome: identification of the insulin-like growth factor (IGF)/IGF-binding protein-3 pathway in acute lung injury. Am J Pathol. 2006;169:86-95 pubmed
Cortesio C, Jiang W. Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5. Arch Biochem Biophys. 2006;449:164-70 pubmed
Nakamura M, Miyamoto S, Maeda H, Ishii G, Hasebe T, Chiba T, et al. Matrix metalloproteinase-7 degrades all insulin-like growth factor binding proteins and facilitates insulin-like growth factor bioavailability. Biochem Biophys Res Commun. 2005;333:1011-6 pubmed
Congote L, Difalco M, Gibbs B. Thrombospondin 1, produced by endothelial cells under the action of erythropoietin, stimulates thymidine incorporation into erythroid cells and counteracts the inhibitory action of insulin-like growth factor binding protein 3. Cytokine. 2005;30:248-53 pubmed
Kirman I, Poltoratskaia N, Sylla P, Whelan R. Insulin-like growth factor-binding protein 3 inhibits growth of experimental colocarcinoma. Surgery. 2004;136:205-9 pubmed

product information

master code :

675-B3

SKU :

675-B3-025

product name :

Recombinant Human IGFBP-3 Protein, CF

unit size :

25 ug

description :

The Recombinant Human IGFBP-3 Protein, CF from R&D Systems is derived from NS0. The Recombinant Human IGFBP-3 Protein, CF has been validated for the following applications: Bioactivity.

target :

IGFBP-3

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in Acetonitrile and TFA.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Human

observed molecular weight :

37-47 kDa, reducing conditions

theoretical molecular weight :

29 kDa

gene symbol :

IGFBP3

details of functionality :

Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF-7 human breast cancer cells. Karey, K.P. et al . (1988) Cancer Research 48 :4083. The ED50 for this effect is 0.05-0.15 ╡g/mL in the presence of 14 ng/mL Recombinant Human IGF-II (Catalog # 292-G2 ).

endotoxin note :

<0.01 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

CAA46087

applications :

Bioactivity

source long :

Mouse myeloma cell line, NS0-derived human IGFBP-3 protein Gly28-Lys291, with an N-terminal Met

source short :

NS0

USD :

407 USD

alt names :

acid stable subunit of the 140 K IGF complex, binding protein 29, binding protein 53, growth hormone-dependent binding protein, IBP-3, IBP3BP-53, IGF-binding protein 3, IGFBP3, IGFBP-3, insulin-like growth factor binding protein 3, insulin-like growth factor-binding protein 3

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage