product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human Semaphorin 5B Fc Chimera Protein, CF
catalog :
6680-S5-050
quantity :
50 ug
price :
523 USD
more info or order :
citations: 1
Reference
Jung J, Zhang K, Wang Z, McMurray M, Tkaczuk A, Ogawa Y, et al. Semaphorin-5B Controls Spiral Ganglion Neuron Branch Refinement During Development. J Neurosci. 2019;: pubmed publisher
product information
master code :
6680-S5
SKU :
6680-S5-050
product name :
Recombinant Human Semaphorin 5B Fc Chimera Protein, CF
unit size :
50 ug
description :
The Recombinant Human Semaphorin 5B Fc Chimera Protein, CF from R&D Systems is derived from CHO. The Recombinant Human Semaphorin 5B Fc Chimera Protein, CF has been validated for the following applications: Bioactivity.
target :
Semaphorin 5B
category :
Proteins and Enzymes
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS.
conjugate :
Unconjugated
purity :
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
species :
Human
observed molecular weight :
130-140 kDa, reducing conditions
theoretical molecular weight :
110.3 kDa (monomer)
gene symbol :
SEMA5B
details of functionality :
Measured by the ability of the immobilized protein to support the adhesion of human pancreatic cancer cells. When 5 x 104 cells/well are added to Recombinant Human Semaphorin 5B Fc Chimera coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour at 37 ░C. The ED50 for this effect is 0.7-2.8 ╡g/mL.
endotoxin note :
<0.10 EU per 1 ╡g of the protein by the LAL method.
accessionNumbers :
NP_001026872
applications :
Bioactivity
source long :
Chinese Hamster Ovary cell line, CHO-derived human Semaphorin 5B protein Human Semaphorin 5B (Val78 - Thr834) Accession # NP_001026872 IEGRMD Human IgG1 (Pro100 - Lys330) N-terminus C-terminus
source short :
CHO
USD :
523 USD
alt names :
FLJ10372, KIAA1445, sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B, SEMA5B, SEMAG, SEMG
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.