Recombinant Human LIGHT/TNFSF14 Protein | R&D Systems 664-LI product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human LIGHT/TNFSF14 Protein

catalog :

664-LI

quantity :

25 ug

price :

329 USD

citations: 18

Reference
Xie C, Jiang B, Qin L, Tellides G, Kirkiles Smith N, Jane Wit D, et al. Complement-activated interferon-γ-primed human endothelium transpresents interleukin-15 to CD8+ T cells. J Clin Invest. 2020;130:3437-3452 pubmed publisher
An S, Raju I, Surenkhuu B, Kwon J, Gulati S, Karaman M, et al. Neutrophil extracellular traps (NETs) contribute to pathological changes of ocular graft-vs.-host disease (oGVHD) dry eye: Implications for novel biomarkers and therapeutic strategies. Ocul Surf. 2019;: pubmed publisher
Kinchen J, Chen H, Parikh K, Antanaviciute A, Jagielowicz M, Fawkner Corbett D, et al. Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel Disease. Cell. 2018;175:372-386.e17 pubmed publisher
Kim K, Choi H, Kim H, Lee T. TNFSF14 inhibits melanogenesis via NF-kB signaling in melanocytes. Cytokine. 2018;110:126-130 pubmed publisher
da Silva Antunes R, Mehta A, Madge L, Tocker J, Croft M. TNFSF14 (LIGHT) Exhibits Inflammatory Activities in Lung Fibroblasts Complementary to IL-13 and TGF-β. Front Immunol. 2018;9:576 pubmed publisher
Clarke A, Poulton L, Shim D, Mabon D, Butt D, Pollard M, et al. An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease. MAbs. 2018;10:664-677 pubmed publisher
Abadpour S, Halvorsen B, Sahraoui A, Korsgren O, Aukrust P, Scholz H. Interleukin-22 reverses human islet dysfunction and apoptosis triggered by hyperglycemia and LIGHT. J Mol Endocrinol. 2018;60:171-183 pubmed publisher
Yuan X, Gu Y, Lai X, Gu Q. LIGHT is increased in patients with coronary disease and regulates inflammatory response and lipid metabolism in oxLDL-induced THP-1 macrophages. Biochem Biophys Res Commun. 2017;490:732-738 pubmed publisher
Sibilano R, Gaudenzio N, DeGorter M, Reber L, Hernandez J, Starkl P, et al. A TNFRSF14-Fc?RI-mast cell pathway contributes to development of multiple features of asthma pathology in mice. Nat Commun. 2016;7:13696 pubmed publisher
Heo S, Noh E, Gwon G, Kim J, Jo J, Choi Y, et al. LIGHT (TNFSF14) Increases the Survival and Proliferation of Human Bone Marrow-Derived Mesenchymal Stem Cells. PLoS ONE. 2016;11:e0166589 pubmed publisher
da Silva Antunes R, Madge L, Soroosh P, Tocker J, Croft M. The TNF Family Molecules LIGHT and Lymphotoxin Î±Î² Induce a Distinct Steroid-Resistant Inflammatory Phenotype in Human Lung Epithelial Cells. J Immunol. 2015;195:2429-41 pubmed publisher
Herro R, Antunes R, Aguilera A, Tamada K, Croft M. The Tumor Necrosis Factor Superfamily Molecule LIGHT Promotes Keratinocyte Activity and Skin Fibrosis. J Invest Dermatol. 2015;135:2109-2118 pubmed publisher
Mikami Y, Matsuzaki H, Horie M, Noguchi S, Jo T, Narumoto O, et al. Lymphotoxin Î² receptor signaling induces IL-8 production in human bronchial epithelial cells. PLoS ONE. 2014;9:e114791 pubmed publisher
Jin H, Jin X, Lee J. Zinc-finger protein 91 plays a key role in LIGHT-induced activation of non-canonical NF-?B pathway. Biochem Biophys Res Commun. 2010;400:581-6 pubmed publisher
Sandberg W, Halvorsen B, Yndestad A, Smith C, Otterdal K, Brosstad F, et al. Inflammatory interaction between LIGHT and proteinase-activated receptor-2 in endothelial cells: potential role in atherogenesis. Circ Res. 2009;104:60-8 pubmed publisher
Kang Y, Kim S, Kang J, Han S, Nam E, Kyung H, et al. LIGHT up-regulated on B lymphocytes and monocytes in rheumatoid arthritis mediates cellular adhesion and metalloproteinase production by synoviocytes. Arthritis Rheum. 2007;56:1106-17 pubmed
Otterdal K, Smith C, Oie E, Pedersen T, Yndestad A, Stang E, et al. Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes. Blood. 2006;108:928-35 pubmed
Edwards J, Sun S, Locklin R, Shipman C, Adamopoulos I, Athanasou N, et al. LIGHT (TNFSF14), a novel mediator of bone resorption, is elevated in rheumatoid arthritis. Arthritis Rheum. 2006;54:1451-62 pubmed

product information

brand :

R&D Systems

master code :

664-LI

SKU :

664-LI-025

product name :

Recombinant Human LIGHT/TNFSF14 Protein

description :

The Recombinant Human LIGHT/TNFSF14 Protein from R&D Systems is derived from NS0. The Recombinant Human LIGHT/TNFSF14 Protein has been validated for the following applications: Bioactivity.

target :

LIGHT/TNFSF14

category :

Proteins and Enzymes

unit size :

25 ug

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

species :

Human

observed molecular weight :

25 kDa, reducing conditions

theoretical molecular weight :

20.9 kDa

gene symbol :

TNFSF14

details of functionality :

Measured in a cytotoxicity assay using HT-29 human colon adenocarcinoma cells Yu, K.Y. et al . (1999) J. Biol. Chem. 274 :13733; Harrop, J.A. et al . (1998) J. Biol. Chem. 273 :27548; Zhai, Y. et al . (1998) J. Clin. Invest. 102 :1142. Recombinant Human LIGHT/TNFSF14 at 10 ng/mL causes a 2-4.5 fold inhibition of HT29 proliferation, in the presence of 10 U/mL (1 ng/mL) Recombinant Human IFN-gamma (Catalog # 285-IF ). Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al . (1990) Proc. Natl. Acad. Sci. USA 87 :1323. The ED50 for this effect is 1-4 ng/mL.

endotoxin note :

<0.10 EU per 1 µg of the protein by the LAL method.

catalog number base :

664-LI

accessionNumbers :

O43557

applications :

Bioactivity

source :

Mouse myeloma cell line, NS0-derived human LIGHT/TNFSF14 protein Met 10-His tag GGGSGGGSGGGSIEGR Human Light (Asp74-Val240) Accession # O43557 N-terminus C-terminus

2020 USD :

329

2021 USD :

329 USD

alt names :

CD258, CD258 antigen, delta transmembrane LIGHT, Herpes virus entry mediator ligand, Herpesvirus entry mediator ligand, HVEM-L, HVEMLherpesvirus entry mediator-ligand, ligand for herpesvirus entry mediator, LIGHTherpesvirus entry mediator A, LTg, TNFSF14, TR2, tumor necrosis factor (ligand) superfamily, member 14, tumor necrosis factor ligand superfamily member 14, tumor necrosis factor receptor-like 2, tumor necrosis factor superfamily member LIGHT

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.