This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
R&D Systems
product type :
protein
product name :
Recombinant Human Agrin Protein, CF
catalog :
6624-AG-050
quantity :
50 ug
price :
524 USD
citations: 10
Reference
Silvestri B, Mochi M, Mawrie D, de Turris V, Colantoni A, Borhy B, et al. HuD impairs neuromuscular junctions and induces apoptosis in human iPSC and Drosophila ALS models. Nat Commun. 2024;15:9618 pubmed publisher
Barbeau S, Semprez F, Dobbertin A, Merriadec L, Roussange F, Eymard B, et al. Molecular Analysis of a Congenital Myasthenic Syndrome Due to a Pathogenic Variant Affecting the C-Terminus of ColQ. Int J Mol Sci. 2023;24: pubmed publisher
Yamashita Y, Nakada S, Nakamura K, Sakurai H, Ohno K, Goto T, et al. Evaluation of Human-Induced Pluripotent Stem Cells Derived from a Patient with Schwartz-Jampel Syndrome Revealed Distinct Hyperexcitability in the Skeletal Muscles. Biomedicines. 2023;11: pubmed publisher
Stoklund Dittlau K, Terrie L, Baatsen P, Kerstens A, De Swert L, Janky R, et al. FUS-ALS hiPSC-derived astrocytes impair human motor units through both gain-of-toxicity and loss-of-support mechanisms. Mol Neurodegener. 2023;18:5 pubmed publisher
Pandya M, Gopinathan G, Tillberg C, Wang J, Luan X, Diekwisch T. The Hippo Pathway Effectors YAP/TAZ Are Essential for Mineralized Tissue Homeostasis in the Alveolar Bone/Periodontal Complex. J Dev Biol. 2022;10: pubmed publisher
Stoklund Dittlau K, Krasnow E, Fumagalli L, Vandoorne T, Baatsen P, Kerstens A, et al. Human motor units in microfluidic devices are impaired by FUS mutations and improved by HDAC6 inhibition. Stem Cell Reports. 2021;16:2213-2227 pubmed publisher
Lin C, Yoshida M, Li L, Ikenaka A, Oshima S, Nakagawa K, et al. iPSC-derived functional human neuromuscular junctions model the pathophysiology of neuromuscular diseases. JCI Insight. 2019;4: pubmed publisher
Nagy N, Barad C, Hotta R, Bhave S, Arciero E, DORA D, et al. Collagen 18 and agrin are secreted by neural crest cells to remodel their microenvironment and regulate their migration during enteric nervous system development. Development. 2018;145: pubmed publisher
Katt M, Linville R, Mayo L, Xu Z, Searson P. Functional brain-specific microvessels from iPSC-derived human brain microvascular endothelial cells: the role of matrix composition on monolayer formation. Fluids Barriers CNS. 2018;15:7 pubmed publisher
Toda M, Ueno M, Yamada J, Hiraga A, Tanaka H, Schlötzer Schrehardt U, et al. The Different Binding Properties of Cultured Human Corneal Endothelial Cell Subpopulations to Descemet's Membrane Components. Invest Ophthalmol Vis Sci. 2016;57:4599-605 pubmed publisher
product information
master code :
6624-AG
SKU :
6624-AG-050
product name :
Recombinant Human Agrin Protein, CF
unit size :
50 ug
description :
The Recombinant Human Agrin Protein, CF from R&D Systems is derived from CHO. The Recombinant Human Agrin Protein, CF has been validated for the following applications: Bioactivity.
target :
Agrin
category :
Proteins and Enzymes
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS.
conjugate :
Unconjugated
purity :
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.
species :
Human
observed molecular weight :
100-110 kDa, reducing conditions
theoretical molecular weight :
83.5 kDa
gene symbol :
AGRN
details of functionality :
Measured by the ability of the immobilized protein to support the adhesion of U-87 MG human glioblastoma/astrocytoma cells. The typical ED50 for this effect is 0.9-3.6 ╡g/mL after 1 hour incubation at 37 ░C. . Optimal dilutions should be determined by each laboratory for each application.
endotoxin note :
<0.01 EU per 1 ╡g of the protein by the LAL method.
accessionNumbers :
NP_940978
applications :
Bioactivity
source long :
Chinese Hamster Ovary cell line, CHO-derived human Agrin protein Ala1260-Pro2045, with an N-terminal 6-His tag
source short :
CHO
USD :
524 USD
alt names :
agrin, agrin proteoglycan, AGRN
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.