This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
R&D Systems
product type :
protein
product name :
Recombinant Human/Mouse Wnt-5a Protein
catalog :
645-WN
quantity :
10 ug
price :
489 USD
citations: 134
Reference
Chen Q, Jiao J, Wang Y, Mai Z, Ren J, He S, et al. Egr-1 mediates low-dose arecoline induced human oral mucosa fibroblast proliferation via transactivation of Wnt5a expression. BMC Mol Cell Biol. 2020;21:80 pubmed publisher
Flores Hernández E, Velázquez D, Castañeda Patlán M, Fuentes García G, Fonseca Camarillo G, Yamamoto Furusho J, et al. Canonical and non-canonical Wnt signaling are simultaneously activated by Wnts in colon cancer cells. Cell Signal. 2020;72:109636 pubmed publisher
Ikezu S, Yeh H, Delpech J, Woodbury M, Van Enoo A, Ruan Z, et al. Inhibition of colony stimulating factor 1 receptor corrects maternal inflammation-induced microglial and synaptic dysfunction and behavioral abnormalities. Mol Psychiatry. 2020;: pubmed publisher
Kozielewicz P, Turku A, Bowin C, Petersen J, Valnohova J, Canizal M, et al. Structural insight into small molecule action on Frizzleds. Nat Commun. 2020;11:414 pubmed publisher
Luo C, Balsa E, Perry E, Liang J, Tavares C, Vazquez F, et al. H3K27me3-mediated PGC1α gene silencing promotes melanoma invasion through WNT5A and YAP. J Clin Invest. 2020;130:853-862 pubmed publisher
de Rezende M, Ng Blichfeldt J, Justo G, Paredes Gamero E, Gosens R. Divergent effects of Wnt5b on IL-3- and GM-CSF-induced myeloid differentiation. Cell Signal. 2020;67:109507 pubmed publisher
Zhao F, Zhou L, Liu J, Xu Z, Ping W, Li H, et al. Construction of a vascularized bladder with autologous adipose-derived stromal vascular fraction cells combined with bladder acellular matrix via tissue engineering. J Tissue Eng. 2019;10:2041731419891256 pubmed publisher
Nagaraja S, Quezada M, Gillespie S, Arzt M, Lennon J, Woo P, et al. Histone Variant and Cell Context Determine H3K27M Reprogramming of the Enhancer Landscape and Oncogenic State. Mol Cell. 2019;76:965-980.e12 pubmed publisher
Wu X, Van Dijk E, Ng Blichfeldt J, Bos I, Ciminieri C, Konigshoff M, et al. Mesenchymal WNT-5A/5B Signaling Represses Lung Alveolar Epithelial Progenitors. Cells. 2019;8: pubmed publisher
Barbero G, Castro M, Villanueva M, Quezada M, Fernández N, Demorrow S, et al. An Autocrine Wnt5a Loop Promotes NF-κB Pathway Activation and Cytokine/Chemokine Secretion in Melanoma. Cells. 2019;8: pubmed publisher
Ji Z, Zhao W, Lin H, Zhou X. Systematically understanding the immunity leading to CRPC progression. PLoS Comput Biol. 2019;15:e1007344 pubmed publisher
Kaiser K, Gyllborg D, Prochazka J, Salasova A, Kompaníková P, Molina F, et al. WNT5A is transported via lipoprotein particles in the cerebrospinal fluid to regulate hindbrain morphogenesis. Nat Commun. 2019;10:1498 pubmed publisher
Lutze G, Haarmann A, Demanou Toukam J, Buttler K, Wilting J, Becker J. Non-canonical WNT-signaling controls differentiation of lymphatics and extension lymphangiogenesis via RAC and JNK signaling. Sci Rep. 2019;9:4739 pubmed publisher
Li W, Yu X, Zhu C, Wang Z, Zhao Z, Li Y, et al. Notum attenuates HBV-related liver fibrosis through inhibiting Wnt 5a mediated non-canonical pathways. Biol Res. 2019;52:10 pubmed publisher
Karvonen H, Perttilä R, Niininen W, Hautanen V, Barker H, Murumägi A, et al. Wnt5a and ROR1 activate non-canonical Wnt signaling via RhoA in TCF3-PBX1 acute lymphoblastic leukemia and highlight new treatment strategies via Bcl-2 co-targeting. Oncogene. 2019;38:3288-3300 pubmed publisher
Zhang S, Zhang H, Ghia E, Huang J, Wu L, Zhang J, et al. Inhibition of chemotherapy resistant breast cancer stem cells by a ROR1 specific antibody. Proc Natl Acad Sci U S A. 2019;116:1370-1377 pubmed publisher
Ogorodnikov A, Levin M, Tattikota S, Tokalov S, Hoque M, Scherzinger D, et al. Transcriptome 3'end organization by PCF11 links alternative polyadenylation to formation and neuronal differentiation of neuroblastoma. Nat Commun. 2018;9:5331 pubmed publisher
Brown B, Connolly G, Mill C, Williams H, Angelini G, Johnson J, et al. Aging differentially modulates the Wnt pro-survival signalling pathways in vascular smooth muscle cells. Aging Cell. 2018;:e12844 pubmed publisher
Heliste J, Jokilammi A, Paatero I, Chakroborty D, Stark C, Savunen T, et al. Receptor tyrosine kinase profiling of ischemic heart identifies ROR1 as a potential therapeutic target. BMC Cardiovasc Disord. 2018;18:196 pubmed publisher
Periasamy P, Tran V, O Neill H. Identification of genes which regulate stroma-dependent in vitro hematopoiesis. PLoS ONE. 2018;13:e0205583 pubmed publisher
Lee S, Shin S, Zhong P, Kim H, Kim D, Kim J, et al. Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes. Nat Commun. 2018;9:3434 pubmed publisher
Bae Y, Kwon J, Kim M, Kim G, Choi S, Oh W, et al. Intracellular Calcium Determines the Adipogenic Differentiation Potential of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells via the Wnt5a/β-Catenin Signaling Pathway. Stem Cells Int. 2018;2018:6545071 pubmed publisher
Andersen P, Tampakakis E, Jimenez D, Kannan S, Miyamoto M, Shin H, et al. Precardiac organoids form two heart fields via Bmp/Wnt signaling. Nat Commun. 2018;9:3140 pubmed publisher
Boyan B, Olivares Navarrete R, Berger M, Hyzy S, Schwartz Z. Role of Wnt11 during Osteogenic Differentiation of Human Mesenchymal Stem Cells on Microstructured Titanium Surfaces. Sci Rep. 2018;8:8588 pubmed publisher
Qian P, De Kumar B, He X, Nolte C, Gogol M, Ahn Y, et al. Retinoid-Sensitive Epigenetic Regulation of the Hoxb Cluster Maintains Normal Hematopoiesis and Inhibits Leukemogenesis. Cell Stem Cell. 2018;22:740-754.e7 pubmed publisher
Nakamura B, Glazier A, Kattah M, Duong B, Jia Y, Campo D, et al. A20 regulates canonical wnt-signaling through an interaction with RIPK4. PLoS ONE. 2018;13:e0195893 pubmed publisher
Hasan M, Yu J, Widhopf G, Rassenti L, Chen L, Shen Z, et al. Wnt5a induces ROR1 to recruit DOCK2 to activate Rac1/2 in chronic lymphocytic leukemia. Blood. 2018;: pubmed publisher
Cho Y, Kang Y, Lee S, Lee Y, Seol S, Lee W, et al. Effect of SFRP5 (Secreted Frizzled-Related Protein 5) on the WNT5A (Wingless-Type Family Member 5A)-Induced Endothelial Dysfunction and Its Relevance With Arterial Stiffness in Human Subjects. Arterioscler Thromb Vasc Biol. 2018;38:1358-1367 pubmed publisher
Li Z, Zhang K, Li X, Pan H, Li S, Chen F, et al. Wnt5a suppresses inflammation-driven intervertebral disc degeneration via a TNF-?/NF-?B-Wnt5a negative-feedback loop. Osteoarthritis Cartilage. 2018;26:966-977 pubmed publisher
Gao B, Ajima R, Yang W, Li C, Song H, Anderson M, et al. Coordinated directional outgrowth and pattern formation by integration of Wnt5a and Fgf signaling in planar cell polarity. Development. 2018;145: pubmed publisher
Takahashi T, Shiraishi A, Murata J. The Coordinated Activities of nAChR and Wnt Signaling Regulate Intestinal Stem Cell Function in Mice. Int J Mol Sci. 2018;19: pubmed publisher
Lin H, Lin H, Chiou Y, Wu C, Chiu W, Tang M. Caveolin-1 down-regulation is required for Wnt5a-Frizzled 2 signalling in Ha-RasV12 -induced cell transformation. J Cell Mol Med. 2018;22:2631-2643 pubmed publisher
Ackers I, Szymanski C, Duckett K, Consitt L, Silver M, Malgor R. Blocking Wnt5a signaling decreases CD36 expression and foam cell formation in atherosclerosis. Cardiovasc Pathol. 2018;34:1-8 pubmed publisher
Tusi B, Wolock S, Weinreb C, Hwang Y, Hidalgo D, Zilionis R, et al. Population snapshots predict early haematopoietic and erythroid hierarchies. Nature. 2018;555:54-60 pubmed publisher
Kokabu S, Rosen V. BMP3 expression by osteoblast lineage cells is regulated by canonical Wnt signaling. FEBS Open Bio. 2018;8:168-176 pubmed publisher
Rosengren T, Larsen L, Pedersen L, Christensen S, Møller L. TSC1 and TSC2 regulate cilia length and canonical Hedgehog signaling via different mechanisms. Cell Mol Life Sci. 2018;75:2663-2680 pubmed publisher
Xu J, Li X, Cole A, Sherman Z, Du W. Reduced Cell Division Control Protein 42 Activity Compromises Hematopoiesis-Supportive Function of Fanconi Anemia Mesenchymal Stromal Cells. Stem Cells. 2018;36:785-795 pubmed publisher
Xiong H, Yan T, Zhang W, Shi F, Jiang X, Wang X, et al. miR-613 inhibits cell migration and invasion by downregulating Daam1 in triple-negative breast cancer. Cell Signal. 2018;44:33-42 pubmed publisher
Baio J, Martinez A, Bailey L, Hasaniya N, Pecaut M, Kearns Jonker M. Spaceflight Activates Protein Kinase C Alpha Signaling and Modifies the Developmental Stage of Human Neonatal Cardiovascular Progenitor Cells. Stem Cells Dev. 2018;: pubmed publisher
Reichman D, Park L, Man L, Redmond D, Chao K, Harvey R, et al. Wnt inhibition promotes vascular specification of embryonic cardiac progenitors. Development. 2018;145: pubmed publisher
Chen Y, Xu H, Lin G. Generation of iPSC-derived limb progenitor-like cells for stimulating phalange regeneration in the adult mouse. Cell Discov. 2017;3:17046 pubmed publisher
Ndoye A, Budina Kolomets A, Kugel C, Webster M, Kaur A, Behera R, et al. ATG5 Mediates a Positive Feedback Loop between Wnt Signaling and Autophagy in Melanoma. Cancer Res. 2017;77:5873-5885 pubmed publisher
Susman M, Karuna E, Kunz R, Gujral T, Cantú A, Choi S, et al. Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the control of cell and tissue behaviors in vertebrates. elife. 2017;6: pubmed publisher
Petersen J, Wright S, Rodriguez D, Matricon P, Lahav N, Vromen A, et al. Agonist-induced dimer dissociation as a macromolecular step in G protein-coupled receptor signaling. Nat Commun. 2017;8:226 pubmed publisher
Steinbusch M, Caron M, Surtel D, Friedrich F, Lausch E, Pruijn G, et al. Expression of RMRP RNA is regulated in chondrocyte hypertrophy and determines chondrogenic differentiation. Sci Rep. 2017;7:6440 pubmed publisher
Paclíková P, Bernatik O, Radaszkiewicz T, Bryja V. The N-Terminal Part of the Dishevelled DEP Domain Is Required for Wnt/β-Catenin Signaling in Mammalian Cells. Mol Cell Biol. 2017;37: pubmed publisher
Strakova K, Matricon P, Yokota C, Arthofer E, Bernatik O, Rodriguez D, et al. The tyrosine Y2502.39 in Frizzled 4 defines a conserved motif important for structural integrity of the receptor and recruitment of Disheveled. Cell Signal. 2017;38:85-96 pubmed publisher
Prgomet Z, Andersson T, Lindberg P. Higher expression of WNT5A protein in oral squamous cell carcinoma compared with dysplasia and oral mucosa with a normal appearance. Eur J Oral Sci. 2017;125:237-246 pubmed publisher
Connacher M, Tay J, Ahn N. Rear-polarized Wnt5a-receptor-actin-myosin-polarity (WRAMP) structures promote the speed and persistence of directional cell migration. Mol Biol Cell. 2017;28:1924-1936 pubmed publisher
Skaria T, Bächli E, Schoedon G. WIF1 prevents Wnt5A mediated LIMK/CFL phosphorylation and adherens junction disruption in human vascular endothelial cells. J Inflamm (Lond). 2017;14:10 pubmed publisher
Bernatik O, Radaszkiewicz T, Behal M, Dave Z, Witte F, Mahl A, et al. A Novel Role for the BMP Antagonist Noggin in Sensitizing Cells to Non-canonical Wnt-5a/Ror2/Disheveled Pathway Activation. Front Cell Dev Biol. 2017;5:47 pubmed publisher
Savvidou I, Khong T, Cuddihy A, McLean C, Horrigan S, Spencer A. β-Catenin Inhibitor BC2059 Is Efficacious as Monotherapy or in Combination with Proteasome Inhibitor Bortezomib in Multiple Myeloma. Mol Cancer Ther. 2017;16:1765-1778 pubmed publisher
Gao Y, Cai A, Xi H, Li J, Xu W, Zhang Y, et al. Ring finger protein 43 associates with gastric cancer progression and attenuates the stemness of gastric cancer stem-like cells via the Wnt-?/catenin signaling pathway. Stem Cell Res Ther. 2017;8:98 pubmed publisher
Vesel M, Rapp J, Feller D, Kiss E, Jaromi L, Meggyes M, et al. ABCB1 and ABCG2 drug transporters are differentially expressed in non-small cell lung cancers (NSCLC) and expression is modified by cisplatin treatment via altered Wnt signaling. Respir Res. 2017;18:52 pubmed publisher
Minegishi K, Hashimoto M, Ajima R, Takaoka K, Shinohara K, Ikawa Y, et al. A Wnt5 Activity Asymmetry and Intercellular Signaling via PCP Proteins Polarize Node Cells for Left-Right Symmetry Breaking. Dev Cell. 2017;40:439-452.e4 pubmed publisher
Kessenbrock K, Smith P, Steenbeek S, Pervolarakis N, Kumar R, Minami Y, et al. Diverse regulation of mammary epithelial growth and branching morphogenesis through noncanonical Wnt signaling. Proc Natl Acad Sci U S A. 2017;114:3121-3126 pubmed publisher
Poobalasingam T, Yates L, Walker S, Pereira M, Gross N, Ali A, et al. Heterozygous Vangl2Looptail mice reveal novel roles for the planar cell polarity pathway in adult lung homeostasis and repair. Dis Model Mech. 2017;10:409-423 pubmed publisher
Behera R, Kaur A, Webster M, Kim S, Ndoye A, Kugel C, et al. Inhibition of Age-Related Therapy Resistance in Melanoma by Rosiglitazone-Mediated Induction of Klotho. Clin Cancer Res. 2017;23:3181-3190 pubmed publisher
Jang J, Jung Y, Chae S, Chung S, Kim S, Yoon Y. WNT/?-catenin pathway modulates the TNF-?-induced inflammatory response in bronchial epithelial cells. Biochem Biophys Res Commun. 2017;484:442-449 pubmed publisher
Schou K, Mogensen J, Morthorst S, Nielsen B, Aleliunaite A, Serra Marques A, et al. KIF13B establishes a CAV1-enriched microdomain at the ciliary transition zone to promote Sonic hedgehog signalling. Nat Commun. 2017;8:14177 pubmed publisher
Jang J, Jung Y, Kim Y, Jho E, Yoon Y. LPS-induced inflammatory response is suppressed by Wnt inhibitors, Dickkopf-1 and LGK974. Sci Rep. 2017;7:41612 pubmed publisher
Baarsma H, Skronska Wasek W, Mutze K, Ciolek F, Wagner D, John Schuster G, et al. Noncanonical WNT-5A signaling impairs endogenous lung repair in COPD. J Exp Med. 2017;214:143-163 pubmed publisher
Farah O, Biechele S, Rossant J, Dufort D. Porcupine-dependent Wnt signaling controls stromal proliferation and endometrial gland maintenance through the action of distinct WNTs. Dev Biol. 2017;422:58-69 pubmed publisher
Himburg H, Doan P, Quarmyne M, Yan X, Sasine J, Zhao L, et al. Dickkopf-1 promotes hematopoietic regeneration via direct and niche-mediated mechanisms. Nat Med. 2017;23:91-99 pubmed publisher
Ando F, Sohara E, Morimoto T, Yui N, Nomura N, Kikuchi E, et al. Wnt5a induces renal AQP2 expression by activating calcineurin signalling pathway. Nat Commun. 2016;7:13636 pubmed publisher
Rapp J, Kiss E, Meggyes M, Szabó Meleg E, Feller D, Smuk G, et al. Increased Wnt5a in squamous cell lung carcinoma inhibits endothelial cell motility. BMC Cancer. 2016;16:915 pubmed
Hu B, Wang Q, Wang Y, Hua S, Sauvé C, Ong D, et al. Epigenetic Activation of WNT5A Drives Glioblastoma Stem Cell Differentiation and Invasive Growth. Cell. 2016;167:1281-1295.e18 pubmed publisher
Prasad C, Chaurasiya S, Guilmain W, Andersson T. WNT5A signaling impairs breast cancer cell migration and invasion via mechanisms independent of the epithelial-mesenchymal transition. J Exp Clin Cancer Res. 2016;35:144 pubmed publisher
Ramirez V, Ramos Fernández E, Henriquez J, Lorenzo A, Inestrosa N. Wnt-5a/Frizzled9 Receptor Signaling through the G?o-G?? Complex Regulates Dendritic Spine Formation. J Biol Chem. 2016;291:19092-107 pubmed publisher
Bader E, Migliorini A, Gegg M, Moruzzi N, Gerdes J, Roscioni S, et al. Identification of proliferative and mature ?-cells in the islets of Langerhans. Nature. 2016;535:430-4 pubmed
Skaria T, Burgener J, Bächli E, Schoedon G. IL-4 Causes Hyperpermeability of Vascular Endothelial Cells through Wnt5A Signaling. PLoS ONE. 2016;11:e0156002 pubmed publisher
Tetreault M, Weinblatt D, Shaverdashvili K, Yang Y, Katz J. KLF4 transcriptionally activates non-canonical WNT5A to control epithelial stratification. Sci Rep. 2016;6:26130 pubmed publisher
Skaria T, Bächli E, Schoedon G. Wnt5A/Ryk signaling critically affects barrier function in human vascular endothelial cells. Cell Adh Migr. 2017;11:24-38 pubmed publisher
Lyros O, Nie L, Moore T, Medda R, Otterson M, Behmaram B, et al. Dysregulation of WNT5A/ROR2 Signaling Characterizes the Progression of Barrett-Associated Esophageal Adenocarcinoma. Mol Cancer Res. 2016;14:647-59 pubmed publisher
Tiwary S, Xu L. FRIZZLED7 Is Required for Tumor Initiation and Metastatic Growth of Melanoma Cells. PLoS ONE. 2016;11:e0147638 pubmed publisher
Horigane S, Ageta Ishihara N, Kamijo S, Fujii H, Okamura M, Kinoshita M, et al. Facilitation of axon outgrowth via a Wnt5a-CaMKK-CaMKIα pathway during neuronal polarization. Mol Brain. 2016;9:8 pubmed publisher
Puvirajesinghe T, Bertucci F, Jain A, Scerbo P, Belotti E, Audebert S, et al. Identification of p62/SQSTM1 as a component of non-canonical Wnt VANGL2-JNK signalling in breast cancer. Nat Commun. 2016;7:10318 pubmed publisher
Zhao D, Xu A, Dai Z, Peng J, Zhu M, Shen J, et al. WNT5A transforms intestinal CD8αα⁺ IELs into an unconventional phenotype with pro-inflammatory features. BMC Gastroenterol. 2015;15:173 pubmed publisher
Henry C, Llamosas E, Knipprath Meszaros A, Schoetzau A, Obermann E, Fuenfschilling M, et al. Targeting the ROR1 and ROR2 receptors in epithelial ovarian cancer inhibits cell migration and invasion. Oncotarget. 2015;6:40310-26 pubmed publisher
Martinez S, Scerbo P, Giordano M, Daulat A, Lhoumeau A, Thomé V, et al. The PTK7 and ROR2 Protein Receptors Interact in the Vertebrate WNT/Planar Cell Polarity (PCP) Pathway. J Biol Chem. 2015;290:30562-72 pubmed publisher
Tu Z, Wang Q, Cui T, Wang J, Ran H, Bao H, et al. Uterine RAC1 via Pak1-ERM signaling directs normal luminal epithelial integrity conducive to on-time embryo implantation in mice. Cell Death Differ. 2016;23:169-81 pubmed publisher
Suryawanshi A, Manoharan I, Hong Y, Swafford D, Majumdar T, Taketo M, et al. Canonical wnt signaling in dendritic cells regulates Th1/Th17 responses and suppresses autoimmune neuroinflammation. J Immunol. 2015;194:3295-304 pubmed publisher
Handorf A, Chamberlain C, Li W. Endogenously produced Indian Hedgehog regulates TGFβ-driven chondrogenesis of human bone marrow stromal/stem cells. Stem Cells Dev. 2015;24:995-1007 pubmed publisher
Ouji Y, Ishizaka S, Nakamura Uchiyama F, Okuzaki D, Yoshikawa M. Partial maintenance and long-term expansion of murine skin epithelial stem cells by Wnt-3a in vitro. J Invest Dermatol. 2015;135:1598-1608 pubmed publisher
Godoy J, Arrázola M, Ordenes D, Silva Alvarez C, Braidy N, Inestrosa N. Wnt-5a ligand modulates mitochondrial fission-fusion in rat hippocampal neurons. J Biol Chem. 2014;289:36179-93 pubmed publisher
Ishizuya Oka A, Kajita M, Hasebe T. Thyroid hormone-regulated Wnt5a/Ror2 signaling is essential for dedifferentiation of larval epithelial cells into adult stem cells in the Xenopus laevis intestine. PLoS ONE. 2014;9:e107611 pubmed publisher
Tsai H, Deng W, Lai W, Chiu W, Yang C, Tsai Y, et al. Wnts enhance neurotrophin-induced neuronal differentiation in adult bone-marrow-derived mesenchymal stem cells via canonical and noncanonical signaling pathways. PLoS ONE. 2014;9:e104937 pubmed publisher
Bainbridge T, DeAlmeida V, Izrael Tomasevic A, Chalouni C, Pan B, Goldsmith J, et al. Evolutionary divergence in the catalytic activity of the CAM-1, ROR1 and ROR2 kinase domains. PLoS ONE. 2014;9:e102695 pubmed publisher
Lee J, Heur M. Interleukin-1?-induced Wnt5a enhances human corneal endothelial cell migration through regulation of Cdc42 and RhoA. Mol Cell Biol. 2014;34:3535-45 pubmed publisher
Many A, Brown A. Both canonical and non-canonical Wnt signaling independently promote stem cell growth in mammospheres. PLoS ONE. 2014;9:e101800 pubmed publisher
Li S, Wang W, Zhang N, Ma T, Zhao C. IL-1? mediates MCP-1 induction by Wnt5a in gastric cancer cells. BMC Cancer. 2014;14:480 pubmed publisher
Linnskog R, Jonsson G, Axelsson L, Prasad C, Andersson T. Interleukin-6 drives melanoma cell motility through p38?-MAPK-dependent up-regulation of WNT5A expression. Mol Oncol. 2014;8:1365-78 pubmed publisher
Smith A, Lewis F, Aquila I, Waring C, Nocera A, Agosti V, et al. Isolation and characterization of resident endogenous c-Kit+ cardiac stem cells from the adult mouse and rat heart. Nat Protoc. 2014;9:1662-81 pubmed publisher
Zhao Y, Wang C, Li R, Hui T, Su Y, Yuan Q, et al. Wnt5a promotes inflammatory responses via nuclear factor ?B (NF-?B) and mitogen-activated protein kinase (MAPK) pathways in human dental pulp cells. J Biol Chem. 2014;289:21028-39 pubmed
Kilander M, Dahlström J, Schulte G. Assessment of Frizzled 6 membrane mobility by FRAP supports G protein coupling and reveals WNT-Frizzled selectivity. Cell Signal. 2014;26:1943-9 pubmed publisher
Ekström E, Bergenfelz C, von Bulow V, Serifler F, Carlemalm E, Jonsson G, et al. WNT5A induces release of exosomes containing pro-angiogenic and immunosuppressive factors from malignant melanoma cells. Mol Cancer. 2014;13:88 pubmed publisher
Bentzinger C, von Maltzahn J, Dumont N, Stark D, Wang Y, Nhan K, et al. Wnt7a stimulates myogenic stem cell motility and engraftment resulting in improved muscle strength. J Cell Biol. 2014;205:97-111 pubmed publisher
Okamoto M, Udagawa N, Uehara S, Maeda K, Yamashita T, Nakamichi Y, et al. Noncanonical Wnt5a enhances Wnt/?-catenin signaling during osteoblastogenesis. Sci Rep. 2014;4:4493 pubmed publisher
Liu A, Chen S, Cai S, Dong L, Liu L, Yang Y, et al. Wnt5a through noncanonical Wnt/JNK or Wnt/PKC signaling contributes to the differentiation of mesenchymal stem cells into type II alveolar epithelial cells in vitro. PLoS ONE. 2014;9:e90229 pubmed publisher
Yu C, Nguyen T, Irvine K, Sweet M, Frazer I, Blumenthal A. Recombinant Wnt3a and Wnt5a elicit macrophage cytokine production and tolerization to microbial stimulation via Toll-like receptor 4. Eur J Immunol. 2014;44:1480-90 pubmed publisher
Zhang A, He S, Sun X, Ding L, Bao X, Wang N. Wnt5a promotes migration of human osteosarcoma cells by triggering a phosphatidylinositol-3 kinase/Akt signals. Cancer Cell Int. 2014;14:15 pubmed publisher
Lee G, Kang D, Ha Y, Jung Y, Chung J, Min K, et al. Prostate cancer bone metastases acquire resistance to androgen deprivation via WNT5A-mediated BMP-6 induction. Br J Cancer. 2014;110:1634-44 pubmed publisher
Keats E, Dominguez J, Grant M, Khan Z. Switch from canonical to noncanonical Wnt signaling mediates high glucose-induced adipogenesis. Stem Cells. 2014;32:1649-60 pubmed publisher
Zhao C, Bu X, Wang W, Ma T, Ma H. GEC-derived SFRP5 inhibits Wnt5a-induced macrophage chemotaxis and activation. PLoS ONE. 2014;9:e85058 pubmed publisher
Ye Z, Zhang C, Tu T, Sun M, Liu D, Lu D, et al. Wnt5a uses CD146 as a receptor to regulate cell motility and convergent extension. Nat Commun. 2013;4:2803 pubmed publisher
Dawes L, Sugiyama Y, Lovicu F, Harris C, Shelley E, McAvoy J. Interactions between lens epithelial and fiber cells reveal an intrinsic self-assembly mechanism. Dev Biol. 2014;385:291-303 pubmed publisher
Thrasivoulou C, Millar M, Ahmed A. Activation of intracellular calcium by multiple Wnt ligands and translocation of ?-catenin into the nucleus: a convergent model of Wnt/Ca2+ and Wnt/?-catenin pathways. J Biol Chem. 2013;288:35651-9 pubmed publisher
Florian M, Nattamai K, Dörr K, Marka G, Uberle B, Vas V, et al. A canonical to non-canonical Wnt signalling switch in haematopoietic stem-cell ageing. Nature. 2013;503:392-6 pubmed publisher
Povinelli B, Nemeth M. Wnt5a regulates hematopoietic stem cell proliferation and repopulation through the Ryk receptor. Stem Cells. 2014;32:105-15 pubmed publisher
Li B, Shi Y, Shu J, Gao J, Wu P, Tang S. Wingless-type mammary tumor virus integration site family, member 5A (Wnt5a) regulates human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein 120 (gp120)-induced expression of pro-inflammatory cytokines via the Ca2+/calmodulin-dependent pr. J Biol Chem. 2013;288:13610-9 pubmed publisher
Cajanek L, Ganji R, Henriques Oliveira C, Theofilopoulos S, Konik P, Bryja V, et al. Tiam1 regulates the Wnt/Dvl/Rac1 signaling pathway and the differentiation of midbrain dopaminergic neurons. Mol Cell Biol. 2013;33:59-70 pubmed publisher
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Tran H, Polakis P. Reversible modification of adenomatous polyposis coli (APC) with K63-linked polyubiquitin regulates the assembly and activity of the ?-catenin destruction complex. J Biol Chem. 2012;287:28552-63 pubmed publisher
Cohen E, Miller M, Wang Z, Moon R, Morrisey E. Wnt5a and Wnt11 are essential for second heart field progenitor development. Development. 2012;139:1931-40 pubmed publisher
Goel S, Chin E, Fakhraldeen S, Berry S, Beebe D, Alexander C. Both LRP5 and LRP6 receptors are required to respond to physiological Wnt ligands in mammary epithelial cells and fibroblasts. J Biol Chem. 2012;287:16454-66 pubmed publisher
Endo M, Doi R, Nishita M, Minami Y. Ror family receptor tyrosine kinases regulate the maintenance of neural progenitor cells in the developing neocortex. J Cell Sci. 2012;125:2017-29 pubmed publisher
Li B, Zhong L, Yang X, Andersson T, Huang M, Tang S. WNT5A signaling contributes to A?-induced neuroinflammation and neurotoxicity. PLoS ONE. 2011;6:e22920 pubmed publisher
Scheel C, Eaton E, Li S, Chaffer C, Reinhardt F, Kah K, et al. Paracrine and autocrine signals induce and maintain mesenchymal and stem cell states in the breast. Cell. 2011;145:926-40 pubmed publisher
Blakely B, Bye C, Fernando C, Horne M, Macheda M, Stacker S, et al. Wnt5a regulates midbrain dopaminergic axon growth and guidance. PLoS ONE. 2011;6:e18373 pubmed publisher
Albers J, Schulze J, Beil F, Gebauer M, Baranowsky A, Keller J, et al. Control of bone formation by the serpentine receptor Frizzled-9. J Cell Biol. 2011;192:1057-72 pubmed publisher
Kilander M, Dijksterhuis J, Ganji R, Bryja V, Schulte G. WNT-5A stimulates the GDP/GTP exchange at pertussis toxin-sensitive heterotrimeric G proteins. Cell Signal. 2011;23:550-4 pubmed publisher
Gudjonsson J, Johnston A, Stoll S, Riblett M, Xing X, Kochkodan J, et al. Evidence for altered Wnt signaling in psoriatic skin. J Invest Dermatol. 2010;130:1849-59 pubmed publisher
Bourhis E, Tam C, Franke Y, Bazan J, Ernst J, Hwang J, et al. Reconstitution of a frizzled8.Wnt3a.LRP6 signaling complex reveals multiple Wnt and Dkk1 binding sites on LRP6. J Biol Chem. 2010;285:9172-9 pubmed publisher
Steele B, Harper M, Macaulay I, Morrell C, Perez Tamayo A, Foy M, et al. Canonical Wnt signaling negatively regulates platelet function. Proc Natl Acad Sci U S A. 2009;106:19836-41 pubmed publisher
O Connell M, Fiori J, Xu M, Carter A, Frank B, Camilli T, et al. The orphan tyrosine kinase receptor, ROR2, mediates Wnt5A signaling in metastatic melanoma. Oncogene. 2010;29:34-44 pubmed publisher
Gogolla N, Galimberti I, Deguchi Y, Caroni P. Wnt signaling mediates experience-related regulation of synapse numbers and mossy fiber connectivities in the adult hippocampus. Neuron. 2009;62:510-25 pubmed publisher
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product information
brand :
R&D Systems
master code :
645-WN
SKU :
645-WN-010
product name :
Recombinant Human/Mouse Wnt-5a Protein
description :
The Recombinant Human/Mouse Wnt-5a Protein from R&D Systems is derived from CHO. The Recombinant Human/Mouse Wnt-5a Protein has been validated for the following applications: Bioactivity.
target :
Wnt-5a
category :
Proteins and Enzymes
unit size :
10 ug
buffer :
Lyophilized from a 0.2 µm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.
conjugate :
Unconjugated
purity :
>80%, by SDS-PAGE under reducing conditions and visualized by silver stain.
species :
Human, Mouse
observed molecular weight :
45 kDa, reducing conditions
theoretical molecular weight :
38 kDa
gene symbol :
WNT5A
details of functionality :
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 0.1-0.5 µg/mL, in the presence of 5 ng/mL rmWnt-3a. Optimal concentrations should be determined by each laboratory for each application.
endotoxin note :
<1.0 EU per 1 µg of the protein by the LAL method.
catalog number base :
645-WN
accessionNumbers :
P22725
applications :
Bioactivity
source :
Chinese Hamster Ovary cell line, CHO-derived Wnt-5a protein Gln38-Lys380
2020 USD :
459
2021 USD :
489 USD
alt names :
hWNT5A, protein Wnt-5a, wingless-type MMTV integration site family, member 5A, Wnt5a, WNT-5A protein
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.