product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human/Mouse Wnt-5a Protein, CF
catalog :
645-WN-010/CF
quantity :
10 ug
price :
601 USD
more info or order :
citations: 162
Reference
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Kilander M, Dahlström J, Schulte G. Assessment of Frizzled 6 membrane mobility by FRAP supports G protein coupling and reveals WNT-Frizzled selectivity. Cell Signal. 2014;26:1943-9 pubmed publisher
Ekström E, Bergenfelz C, von Bulow V, Serifler F, Carlemalm E, Jonsson G, et al. WNT5A induces release of exosomes containing pro-angiogenic and immunosuppressive factors from malignant melanoma cells. Mol Cancer. 2014;13:88 pubmed publisher
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Zhang A, He S, Sun X, Ding L, Bao X, Wang N. Wnt5a promotes migration of human osteosarcoma cells by triggering a phosphatidylinositol-3 kinase/Akt signals. Cancer Cell Int. 2014;14:15 pubmed publisher
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image
image 1 :
R&D Systems 645-WN-010/CF image 1
Graph showing dose-dependent bioactivity of mouse Wnt-5a protein
product information
brand :
R&D Systems
master code :
645-WN/CF
SKU :
645-WN-010/CF
product name :
Recombinant Human/Mouse Wnt-5a Protein, CF
description :
The Recombinant Human/Mouse Wnt-5a Protein, CF from R&D Systems is derived from CHO. The Recombinant Human/Mouse Wnt-5a Protein, CF has been validated for the following applications: Bioactivity.
target :
Wnt-5a
category :
Proteins and Enzymes
unit sizes :
10 ug
buffer :
Lyophilized from a 0.2 µm filtered solution in PBS, EDTA and CHAPS.
conjugate :
Unconjugated
purity :
>80%, by SDS-PAGE under reducing conditions and visualized by silver stain.
species :
Human, Mouse
observed molecular weight :
45 kDa, reducing conditions
theoretical molecular weight :
38 kDa
gene symbol :
WNT5A
details of functionality :
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 0.1-0.5 µg/mL, in the presence of 5 ng/mL rmWnt-3a. Optimal concentrations should be determined by each laboratory for each application.
endotoxin note :
<1.0 EU per 1 µg of the protein by the LAL method.
accessionNumbers :
P22725
applications :
Bioactivity
source :
CHO-derived Recombinant Human/Mouse Wnt-5a Protein, CF
USD :
601 USD
alt names :
hWNT5A, protein Wnt-5a, wingless-type MMTV integration site family, member 5A, Wnt5a, WNT-5A protein
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.