Recombinant Human CD40 Ligand/TNFSF5 (HEK293-expressed), CF | R&D Systems 6420-CLB-050 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human CD40 Ligand/TNFSF5 (HEK293-expressed), CF

catalog :

6420-CLB-050

quantity :

50 ug

price :

369 USD

more info or order :

R&D Systems product webpage

citations: 19

Reference
Mebrahtu A, Laur xe9 n I, Veerman R, Akpinar G, Lord M, Kostakis A, et al. A bispecific CD40 agonistic antibody allowing for antibody-peptide conjugate formation to enable cancer-specific peptide delivery, resulting in improved T proliferation and anti-tumor immunity in mice. Nat Commun. 2024;15:9542 pubmed publisher
Doba xf1 o L xf3 pez C, Valero J, Araujo Ayala F, Nadeu F, Gava F, Faria C, et al. Patient-derived follicular lymphoma spheroids recapitulate lymph node signaling and immune profile uncovering galectin-9 as a novel immunotherapeutic target. Blood Cancer J. 2024;14:75 pubmed publisher
Leylek R, Alcántara Hernández M, Granja J, Chavez M, Perez K, Diaz O, et al. Chromatin Landscape Underpinning Human Dendritic Cell Heterogeneity. Cell Rep. 2020;32:108180 pubmed publisher
Bibby J, Purvis H, Hayday T, Chandra A, Okkenhaug K, Rosenzweig S, et al. Cholesterol metabolism drives regulatory B cell IL-10 through provision of geranylgeranyl pyrophosphate. Nat Commun. 2020;11:3412 pubmed publisher
Kojok K, Mohsen M, El Kadiry A, Mourad W, Merhi Y. Aspirin Reduces the Potentiating Effect of CD40L on Platelet Aggregation via Inhibition of Myosin Light Chain. J Am Heart Assoc. 2020;9:e013396 pubmed publisher
Hasan M, Thompson Snipes L, Klintmalm G, Demetris A, O Leary J, Oh S, et al. CD24hiCD38hi and CD24hiCD27+ Human Regulatory B Cells Display Common and Distinct Functional Characteristics. J Immunol. 2019;203:2110-2120 pubmed publisher
Anzilotti C, Swan D, Boisson B, Deobagkar Lele M, Oliveira C, Chabosseau P, et al. An essential role for the Zn2+ transporter ZIP7 in B cell development. Nat Immunol. 2019;20:350-361 pubmed publisher
Bian Z, Field M, Elner S, Kahlenberg J, Elner V. Distinct CD40L receptors mediate inflammasome activation and secretion of IL-1β and MCP-1 in cultured human retinal pigment epithelial cells. Exp Eye Res. 2018;170:29-39 pubmed publisher
Wilson R, Makuch M, Kienzler A, Varley J, Taylor J, Woodhall M, et al. Condition-dependent generation of aquaporin-4 antibodies from circulating B cells in neuromyelitis optica. Brain. 2018;141:1063-1074 pubmed publisher
Clarke A, Poulton L, Shim D, Mabon D, Butt D, Pollard M, et al. An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease. MAbs. 2018;10:664-677 pubmed publisher
Dai J, Luftig M. Intracellular BH3 Profiling Reveals Shifts in Antiapoptotic Dependency in Human B Cell Maturation and Mitogen-Stimulated Proliferation. J Immunol. 2018;200:1727-1736 pubmed publisher
Grossman L, Chang C, Dai J, Nikitin P, Jima D, Dave S, et al. Epstein-Barr Virus Induces Adhesion Receptor CD226 (DNAM-1) Expression during Primary B-Cell Transformation into Lymphoblastoid Cell Lines. mSphere. 2017;2: pubmed publisher
Brach D, Johnston Blackwell D, Drew A, Lingaraj T, Motwani V, Warholic N, et al. EZH2 Inhibition by Tazemetostat Results in Altered Dependency on B-cell Activation Signaling in DLBCL. Mol Cancer Ther. 2017;16:2586-2597 pubmed publisher
Guo Y, Walsh A, Fearon U, Smith M, Wechalekar M, Yin X, et al. CD40L-Dependent Pathway Is Active at Various Stages of Rheumatoid Arthritis Disease Progression. J Immunol. 2017;198:4490-4501 pubmed publisher
Price A, Dai J, Bazot Q, Patel L, Nikitin P, Djavadian R, et al. Epstein-Barr virus ensures B cell survival by uniquely modulating apoptosis at early and late times after infection. elife. 2017;6: pubmed publisher
Liu B, Cao Y, Huizinga T, Hafler D, Toes R. TLR-mediated STAT3 and ERK activation controls IL-10 secretion by human B cells. Eur J Immunol. 2014;44:2121-9 pubmed publisher
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Ramgolam V, Sha Y, Marcus K, Choudhary N, Troiani L, Chopra M, et al. B cells as a therapeutic target for IFN-? in relapsing-remitting multiple sclerosis. J Immunol. 2011;186:4518-26 pubmed publisher

product information

master code :

6420-CLB

SKU :

6420-CLB-050

product name :

Recombinant Human CD40 Ligand/TNFSF5 (HEK293-expressed), CF

unit size :

50 ug

description :

The Recombinant Human CD40 Ligand/TNFSF5 (HEK293-expressed), CF from R&D Systems is derived from HEK293. The Recombinant Human CD40 Ligand/TNFSF5 (HEK293-expressed), CF has been validated for the following applications: Bioactivity.

target :

CD40 Ligand/TNFSF5

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS and EDTA with Trehalose.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

species :

Human

observed molecular weight :

20-27 kDa, under reducing conditions.

theoretical molecular weight :

22 kDa

details of functionality :

Measured in a cell proliferation assay using human B cells. The ED50 for this effect is 0.250-3.00 ng/mL in the presence of Recombinant Human IL-4 (Catalog # 204-IL ) and a cross linking HA Tag Antibody (Catalog # MAB060 ).

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

P29965.1

applications :

Bioactivity

source long :

Human embryonic kidney cell, HEK293-derived human CD40 Ligand/TNFSF5 protein YPYDVPDYA GCN4-IZ GGGSGGGSGGGS Human CD40 Ligand (Met113-Leu261) Accession # P29965.1 N-terminus C-terminus

source short :

HEK293

extended description :

Analyzed by SEC-MALS. HA tag

USD :

369 USD

product details :

Analyzed by SEC-MALS. HA tag

alt names :

CD154, CD154 antigen, CD40 antigen ligand, CD40 ligand, CD40L, CD40-L, CD40LG, CD40LIGM, gp39, hCD40L, HIGM1, T-B cell-activating molecule, T-BAM, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TNFSF5IMD3, TRAP, TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome), tumor necrosis factor (ligand) superfamily member 5, Tumor necrosis factor ligand superfamily member 5

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage