Recombinant Human CD40 Ligand/TNFSF5 (HEK293-expressed) | R&D Systems 6420-CL product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human CD40 Ligand/TNFSF5 (HEK293-expressed)

catalog :

6420-CL

quantity :

25 ug

price :

299 USD

citations: 14

Reference
Hasan M, Thompson Snipes L, Klintmalm G, Demetris A, O Leary J, Oh S, et al. CD24hiCD38hi and CD24hiCD27+ Human Regulatory B Cells Display Common and Distinct Functional Characteristics. J Immunol. 2019;203:2110-2120 pubmed publisher
Anzilotti C, Swan D, Boisson B, Deobagkar Lele M, Oliveira C, Chabosseau P, et al. An essential role for the Zn2+ transporter ZIP7 in B cell development. Nat Immunol. 2019;20:350-361 pubmed publisher
Bian Z, Field M, Elner S, Kahlenberg J, Elner V. Distinct CD40L receptors mediate inflammasome activation and secretion of IL-1β and MCP-1 in cultured human retinal pigment epithelial cells. Exp Eye Res. 2018;170:29-39 pubmed publisher
Wilson R, Makuch M, Kienzler A, Varley J, Taylor J, Woodhall M, et al. Condition-dependent generation of aquaporin-4 antibodies from circulating B cells in neuromyelitis optica. Brain. 2018;141:1063-1074 pubmed publisher
Clarke A, Poulton L, Shim D, Mabon D, Butt D, Pollard M, et al. An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease. MAbs. 2018;10:664-677 pubmed publisher
Dai J, Luftig M. Intracellular BH3 Profiling Reveals Shifts in Antiapoptotic Dependency in Human B Cell Maturation and Mitogen-Stimulated Proliferation. J Immunol. 2018;200:1727-1736 pubmed publisher
Grossman L, Chang C, Dai J, Nikitin P, Jima D, Dave S, et al. Epstein-Barr Virus Induces Adhesion Receptor CD226 (DNAM-1) Expression during Primary B-Cell Transformation into Lymphoblastoid Cell Lines. mSphere. 2017;2: pubmed publisher
Brach D, Johnston Blackwell D, Drew A, Lingaraj T, Motwani V, Warholic N, et al. EZH2 Inhibition by Tazemetostat Results in Altered Dependency on B-cell Activation Signaling in DLBCL. Mol Cancer Ther. 2017;16:2586-2597 pubmed publisher
Guo Y, Walsh A, Fearon U, Smith M, Wechalekar M, Yin X, et al. CD40L-Dependent Pathway Is Active at Various Stages of Rheumatoid Arthritis Disease Progression. J Immunol. 2017;198:4490-4501 pubmed publisher
Price A, Dai J, Bazot Q, Patel L, Nikitin P, Djavadian R, et al. Epstein-Barr virus ensures B cell survival by uniquely modulating apoptosis at early and late times after infection. elife. 2017;6: pubmed publisher
Liu B, Cao Y, Huizinga T, Hafler D, Toes R. TLR-mediated STAT3 and ERK activation controls IL-10 secretion by human B cells. Eur J Immunol. 2014;44:2121-9 pubmed publisher
Nikitin P, Price A, McFadden K, Yan C, Luftig M. Mitogen-induced B-cell proliferation activates Chk2-dependent G1/S cell cycle arrest. PLoS ONE. 2014;9:e87299 pubmed publisher
Wilson T, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, et al. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature. 2012;487:505-9 pubmed publisher
Ramgolam V, Sha Y, Marcus K, Choudhary N, Troiani L, Chopra M, et al. B cells as a therapeutic target for IFN-? in relapsing-remitting multiple sclerosis. J Immunol. 2011;186:4518-26 pubmed publisher

product information

brand :

R&D Systems

master code :

6420-CL

SKU :

6420-CL-025

product name :

Recombinant Human CD40 Ligand/TNFSF5 (HEK293-expressed)

description :

The Recombinant Human CD40 Ligand/TNFSF5 (HEK293-expressed) from R&D Systems is derived from HEK293. The Recombinant Human CD40 Ligand/TNFSF5 (HEK293-expressed) has been validated for the following applications: Bioactivity.

target :

CD40 Ligand/TNFSF5

category :

Proteins and Enzymes

unit size :

25 ug

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS and EDTA with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

species :

Human

observed molecular weight :

22-26 kDa, reducing conditions

theoretical molecular weight :

21.6 kDa

gene symbol :

CD40LG

details of functionality :

Measured in a cell proliferation assay using enriched human B cells in the presence of IL-4. Spriggs, M.K. et al . (1992) J. Exp. Med. 176 :1543. The ED50 for this effect is 0.2-1.2 ng/mL in the presence of 20 ng/mL of Recombinant Human IL-4 (Catalog # 204-IL ) and a cross-linking antibody, Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody (Catalog # MAB060 ). Measured by its ability to induce IL-6 secretion by enriched human B cells. Urashima, M. et al . (1996) Leukemia Res. 20 :507. The ED50 for this effect is approximately 0.5 ng/mL in the presence of Recombinant Human IL-4 (Catalog # 204-IL ) and a cross-linking antibody, Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody (Catalog # MAB060 ).

endotoxin note :

<0.01 EU per 1 µg of the protein by the LAL method.

catalog number base :

6420-CL

accessionNumbers :

P29965

applications :

Bioactivity

source :

Human embryonic kidney cell, HEK293-derived human CD40 Ligand/TNFSF5 protein Hemagglutinin tag YPYDVPDYA GCN4-IZ (Met1-Ile29) GGGSGGGSGGGS Human CD40 Ligand (Met113-Leu261) Accession # P29965 N-terminus C-terminus

2020 USD :

299

2021 USD :

299 USD

alt names :

CD154, CD154 antigen, CD40 antigen ligand, CD40 ligand, CD40L, CD40-L, CD40LG, CD40LIGM, gp39, hCD40L, HIGM1, T-B cell-activating molecule, T-BAM, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TNFSF5IMD3, TRAP, TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome), tumor necrosis factor (ligand) superfamily member 5, Tumor necrosis factor ligand superfamily member 5

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.