Recombinant Human sFRP-5 Protein, CF | R&D Systems 6266-SF-050 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human sFRP-5 Protein, CF

catalog :

6266-SF-050

quantity :

50 ug

price :

541 USD

more info or order :

R&D Systems product webpage

citations: 5

Reference
Sahabian A, Sgodda M, Naujok O, Dettmer R, Dahlmann J, Manstein F, et al. Chemically-Defined, Xeno-Free, Scalable Production of hPSC-Derived Definitive Endoderm Aggregates with Multi-Lineage Differentiation Potential. Cells. 2019;8: pubmed publisher
Au D, Migliorini M, Strickland D, Muratoglu S. Macrophage LRP1 Promotes Diet-Induced Hepatic Inflammation and Metabolic Dysfunction by Modulating Wnt Signaling. Mediators Inflamm. 2018;2018:7902841 pubmed publisher
Cho Y, Kang Y, Lee S, Lee Y, Seol S, Lee W, et al. Effect of SFRP5 (Secreted Frizzled-Related Protein 5) on the WNT5A (Wingless-Type Family Member 5A)-Induced Endothelial Dysfunction and Its Relevance With Arterial Stiffness in Human Subjects. Arterioscler Thromb Vasc Biol. 2018;38:1358-1367 pubmed publisher
Carstensen M, Wiza C, Röhrig K, Fahlbusch P, Roden M, Herder C, et al. Effect of Sfrp5 on cytokine release and insulin action in primary human adipocytes and skeletal muscle cells. PLoS ONE. 2014;9:e85906 pubmed publisher
Wilson T, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, et al. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature. 2012;487:505-9 pubmed publisher

product information

master code :

6266-SF

SKU :

6266-SF-050

product name :

Recombinant Human sFRP-5 Protein, CF

unit size :

50 ug

description :

The Recombinant Human sFRP-5 Protein, CF from R&D Systems is derived from CHO. The Recombinant Human sFRP-5 Protein, CF has been validated for the following applications: Bioactivity.

target :

sFRP-5

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS and NaCl.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

species :

Human

observed molecular weight :

30-40 kDa, reducing conditions

theoretical molecular weight :

33.8, 32.7 30.7 kDa

gene symbol :

SFRP5

details of functionality :

Measured by its ability to inhibit Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 0.2-2 ╡g/mL in the presence of 750 ng/mL Recombinant Human Wnt-3a (Catalog # 5036-WN ).

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

Q5T4F7

applications :

Bioactivity

source long :

Chinese Hamster Ovary cell line, CHO-derived human sFRP-5 protein Glu30-His317, with an N-terminal HA (YPYDVPDYA) tag

source short :

CHO

USD :

541 USD

alt names :

frizzled-related protein 1b, FRP-1b, FRP1B, SARP3, SARP-3, secreted apoptosis related protein 3, secreted apoptosis-related protein 3, secreted frizzled-related protein 5, sFRP5, sFRP-5

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage