Recombinant Human Angiopoietin-2 Protein, CF | R&D Systems 623-AN product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Angiopoietin-2 Protein, CF

catalog :

623-AN

quantity :

1 mg

price :

4650 USD

citations: 38

Reference
THACKABERRY E, Zhou Y, Zuch de Zafra C, Fuh G, Lee C, Sanowar S, et al. Rapid Development of Glaucoma Via ITV Nonselective ANGPT 1/2 Antibody: A Potential Role for ANGPT/TIE2 Signaling in Primate Aqueous Humor Outflow. Invest Ophthalmol Vis Sci. 2019;60:4097-4108 pubmed publisher
Kim J, Park J, Choi J, Park I, Hwang Y, Bae H, et al. Tie2 activation promotes choriocapillary regeneration for alleviating neovascular age-related macular degeneration. Sci Adv. 2019;5:eaau6732 pubmed publisher
Yang X, Chen Z, Meng X, Sun C, Li M, Shu L, et al. Angiopoietin-2 promotes osteogenic differentiation of thoracic ligamentum flavum cells via modulating the Notch signaling pathway. PLoS ONE. 2018;13:e0209300 pubmed publisher
Elamaa H, Kihlström M, Kapiainen E, Kaakinen M, Miinalainen I, Ragauskas S, et al. Angiopoietin-4-dependent venous maturation and fluid drainage in the peripheral retina. elife. 2018;7: pubmed publisher
Lavoie S, Dumas E, Vulesevic B, Neagoe P, White M, Sirois M. Synthesis of Human Neutrophil Extracellular Traps Contributes to Angiopoietin-Mediated In Vitro Proinflammatory and Proangiogenic Activities. J Immunol. 2018;200:3801-3813 pubmed publisher
Wang M, Chen D, Chen L, Cao G, Zhao H, Liu D, et al. Novel inhibitors of the cellular renin-angiotensin system components, poricoic acids, target Smad3 phosphorylation and Wnt/β-catenin pathway against renal fibrosis. Br J Pharmacol. 2018;175:2689-2708 pubmed publisher
Conroy S, Kruyt F, Wagemakers M, Bhat K, den Dunnen W. IL-8 associates with a pro-angiogenic and mesenchymal subtype in glioblastoma. Oncotarget. 2018;9:15721-15731 pubmed publisher
Wang K, Kang L, Liu W, Song Y, Wu X, Zhang Y, et al. Angiopoietin-2 promotes extracellular matrix degradation in human degenerative nucleus pulposus cells. Int J Mol Med. 2018;41:3551-3558 pubmed publisher
Souma T, Thomson B, Heinen S, Carota I, Yamaguchi S, Onay T, et al. Context-dependent functions of angiopoietin 2 are determined by the endothelial phosphatase VEPTP. Proc Natl Acad Sci U S A. 2018;115:1298-1303 pubmed publisher
La Porta S, Roth L, Singhal M, Mogler C, Spegg C, Schieb B, et al. Endothelial Tie1-mediated angiogenesis and vascular abnormalization promote tumor progression and metastasis. J Clin Invest. 2018;128:834-845 pubmed publisher
Daly C, Qian X, Castanaro C, Pasnikowski E, Jiang X, Thomson B, et al. Angiopoietins bind thrombomodulin and inhibit its function as a thrombin cofactor. Sci Rep. 2018;8:505 pubmed publisher
Bohn K, Adkins C, Nounou M, Lockman P. Inhibition of VEGF and Angiopoietin-2 to Reduce Brain Metastases of Breast Cancer Burden. Front Pharmacol. 2017;8:193 pubmed publisher
Wang K, Liu W, Song Y, Wu X, Zhang Y, Li S, et al. The role of angiopoietin-2 in nucleus pulposus cells during human intervertebral disc degeneration. Lab Invest. 2017;97:971-982 pubmed publisher
Morooka N, Futaki S, Sato Nishiuchi R, Nishino M, Totani Y, Shimono C, et al. Polydom Is an Extracellular Matrix Protein Involved in Lymphatic Vessel Remodeling. Circ Res. 2017;120:1276-1288 pubmed publisher
Wu X, Giobbie Hurder A, Liao X, Connelly C, Connolly E, Li J, et al. Angiopoietin-2 as a Biomarker and Target for Immune Checkpoint Therapy. Cancer Immunol Res. 2017;5:17-28 pubmed publisher
Bezabeh B, Fleming R, Fazenbaker C, Zhong H, Coffman K, Yu X, et al. Insertion of scFv into the hinge domain of full-length IgG1 monoclonal antibody results in tetravalent bispecific molecule with robust properties. MAbs. 2017;9:240-256 pubmed publisher
Hossain M, Shifat R, Johnson D, Bedford M, Gabrusiewicz K, Cortes Santiago N, et al. TIE2-mediated tyrosine phosphorylation of H4 regulates DNA damage response by recruiting ABL1. Sci Adv. 2016;2:e1501290 pubmed
Dumas E, Neagoe P, McDonald P, White M, Sirois M. New Insights into the Pro-Inflammatory Activities of Ang1 on Neutrophils: Induction of MIP-1? Synthesis and Release. PLoS ONE. 2016;11:e0163140 pubmed publisher
Jenny Zhou H, Qin L, Zhang H, Tang W, Ji W, He Y, et al. Endothelial exocytosis of angiopoietin-2 resulting from CCM3 deficiency contributes to cerebral cavernous malformation. Nat Med. 2016;22:1033-1042 pubmed publisher
Korhonen E, Lampinen A, Giri H, Anisimov A, Kim M, Allen B, et al. Tie1 controls angiopoietin function in vascular remodeling and inflammation. J Clin Invest. 2016;126:3495-510 pubmed publisher
Chan W, Ismail H, Mayaki D, Sanchez V, Tiedemann K, Davis E, et al. Fibulin-5 Regulates Angiopoietin-1/Tie-2 Receptor Signaling in Endothelial Cells. PLoS ONE. 2016;11:e0156994 pubmed publisher
Rautiola J, Lampinen A, Mirtti T, Ristimaki A, Joensuu H, Bono P, et al. Association of Angiopoietin-2 and Ki-67 Expression with Vascular Density and Sunitinib Response in Metastatic Renal Cell Carcinoma. PLoS ONE. 2016;11:e0153745 pubmed publisher
Kloepper J, Riedemann L, Amoozgar Z, Seano G, Susek K, Yu V, et al. Ang-2/VEGF bispecific antibody reprograms macrophages and resident microglia to anti-tumor phenotype and prolongs glioblastoma survival. Proc Natl Acad Sci U S A. 2016;113:4476-81 pubmed publisher
Hakanpaa L, Sipilä T, Leppänen V, Gautam P, Nurmi H, Jacquemet G, et al. Endothelial destabilization by angiopoietin-2 via integrin Î²1 activation. Nat Commun. 2015;6:5962 pubmed publisher
Brunckhorst M, Xu Y, Lu R, Yu Q. Angiopoietins promote ovarian cancer progression by establishing a procancer microenvironment. Am J Pathol. 2014;184:2285-96 pubmed publisher
Haddad L, Sirois M. Angiopoietin-1 upregulates de novo expression of IL-1? and Il1-Ra, and the exclusive release of Il1-Ra from human neutrophils. PLoS ONE. 2014;9:e88980 pubmed publisher
Keats E, Dominguez J, Grant M, Khan Z. Switch from canonical to noncanonical Wnt signaling mediates high glucose-induced adipogenesis. Stem Cells. 2014;32:1649-60 pubmed publisher
Brindle N, Sale J, Arakawa H, Buerstedde J, Nuamchit T, Sharma S, et al. Directed evolution of an angiopoietin-2 ligand trap by somatic hypermutation and cell surface display. J Biol Chem. 2013;288:33205-12 pubmed publisher
Yu X, Seegar T, Dalton A, Tzvetkova Robev D, Goldgur Y, Rajashankar K, et al. Structural basis for angiopoietin-1-mediated signaling initiation. Proc Natl Acad Sci U S A. 2013;110:7205-10 pubmed publisher
Brunckhorst M, Wang H, Lu R, Yu Q. Angiopoietin-4 promotes glioblastoma progression by enhancing tumor cell viability and angiogenesis. Cancer Res. 2010;70:7283-93 pubmed publisher
Reikvam H, Hatfield K, Oyan A, Kalland K, Kittang A, Bruserud O. Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation. Eur J Haematol. 2010;84:239-51 pubmed publisher
Soman C, Giorgio T. Sensitive and multiplexed detection of proteomic antigens via quantum dot aggregation. Nanomedicine. 2009;5:402-9 pubmed publisher
Saharinen P, Eklund L, Miettinen J, Wirkkala R, Anisimov A, Winderlich M, et al. Angiopoietins assemble distinct Tie2 signalling complexes in endothelial cell-cell and cell-matrix contacts. Nat Cell Biol. 2008;10:527-37 pubmed publisher
Soman C, Giorgio T. Quantum dot self-assembly for protein detection with sub-picomolar sensitivity. Langmuir. 2008;24:4399-404 pubmed publisher
Rao S, Lobov I, Vallance J, Tsujikawa K, Shiojima I, Akunuru S, et al. Obligatory participation of macrophages in an angiopoietin 2-mediated cell death switch. Development. 2007;134:4449-58 pubmed
Vart R, Nikitenko L, Lagos D, Trotter M, Cannon M, Bourboulia D, et al. Kaposi's sarcoma-associated herpesvirus-encoded interleukin-6 and G-protein-coupled receptor regulate angiopoietin-2 expression in lymphatic endothelial cells. Cancer Res. 2007;67:4042-51 pubmed
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product information

brand :

R&D Systems

master code :

623-AN

SKU :

623-AN-01M/CF

product name :

Recombinant Human Angiopoietin-2 Protein, CF

description :

The Recombinant Human Angiopoietin-2 Protein, CF from R&D Systems is derived from NS0. The Recombinant Human Angiopoietin-2 Protein, CF has been validated for the following applications: Bioactivity.

target :

Angiopoietin-2

category :

Proteins and Enzymes

unit size :

1 mg

buffer :

Lyophilized from a 0.2 µm filtered solution in MOPS, NaCl, CHAPS and PEG. *1 MG pack size is supplied as a 0.2 µm filtered solution in MOPS, NaCl, CHAPS and PEG.

conjugate :

Unconjugated

purity :

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

species :

Human

observed molecular weight :

66 kDa, reducing conditions

theoretical molecular weight :

56 kDa

gene symbol :

ANGPT2

details of functionality :

Measured by its ability to activate Tie-2 in C6 rat glial cells transfected with human Tie-2. 0.2 µg/mL of Recombinant Human Angiopoietin-2 significantly induces phosphorylation of human Tie-2. Measured by its binding ability in a functional ELISA. When Recombinant Human Tie-2 Fc Chimera (Catalog # 313-TI) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Angiopoietin-2 binds with an ED50 of 0.5-4 ng/mL.

endotoxin note :

<0.10 EU per 1 µg of the protein by the LAL method.

catalog number base :

623-AN/CF

accessionNumbers :

O15123

applications :

Bioactivity

source :

Mouse myeloma cell line, NS0-derived human Angiopoietin-2 protein Tyr19-Phe496, with a C-terminal 6-His tag

2020 USD :

4650

2021 USD :

4650 USD

alt names :

AGPT2, ANG2, ANG-2, angiopoietin 2, angiopoietin-2, angiopoietin-2a, angiopoietin-2B, angiopoitin 2, ANGPT2, Tie2-ligand

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after reconstitution. 1 month, 2 to 8 °C under sterile conditions after reconstitution.