Recombinant Human Frizzled-8 Fc Chimera Protein, CF | R&D Systems 6129-FZ-050 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Frizzled-8 Fc Chimera Protein, CF

catalog :

6129-FZ-050

quantity :

50 ug

price :

498 USD

more info or order :

R&D Systems product webpage

citations: 4

Reference
Chidiac R, Abedin M, Macleod G, Yang A, Thibeault P, Blazer L, et al. A Norrin/Wnt surrogate antibody stimulates endothelial cell barrier function and rescues retinopathy. EMBO Mol Med. 2021;13:e13977 pubmed publisher
Saito Diaz K, Benchabane H, Tiwari A, Tian A, Li B, Thompson J, et al. APC Inhibits Ligand-Independent Wnt Signaling by the Clathrin Endocytic Pathway. Dev Cell. 2018;44:566-581.e8 pubmed publisher
Jackson H, Granger D, Jones G, Anderson L, Friel S, Rycroft D, et al. Novel Bispecific Domain Antibody to LRP6 Inhibits Wnt and R-spondin Ligand-Induced Wnt Signaling and Tumor Growth. Mol Cancer Res. 2016;14:859-68 pubmed publisher
Wilson T, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, et al. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature. 2012;487:505-9 pubmed publisher

product information

master code :

6129-FZ

SKU :

6129-FZ-050

product name :

Recombinant Human Frizzled-8 Fc Chimera Protein, CF

unit size :

50 ug

description :

The Recombinant Human Frizzled-8 Fc Chimera Protein, CF from R&D Systems is derived from CHO. The Recombinant Human Frizzled-8 Fc Chimera Protein, CF has been validated for the following applications: Bioactivity.

target :

Frizzled-8

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

species :

Human

observed molecular weight :

55-65 kDa, reducing conditions

theoretical molecular weight :

43.3 kDa (monomer)

gene symbol :

FZD8

details of functionality :

Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 15-75 ng/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN ) Measured by its binding ability in a functional ELISA. In a 100 ╡L reaction mixture containing biotinylated recombinant mouse (rm) Wnt-3a or rmWnt-5a at 50-100 ng/mL and Recombinant Human (rh) Frizzled-8 Fc Chimera dilutions at 0.1-8,000 ng/mL, the concentration of rhFrizzled-8 Fc Chimera that produces 50% of the optimal binding response is found to be approximately 7-28 ng/mL. Optimal concentrations should be determined by each laboratory for each application.

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

Q9H461

applications :

Bioactivity

source long :

Chinese Hamster Ovary cell line, CHO-derived human Frizzled-8 protein Human Frizzled-8 (Ala25-Pro172) Accession # Q9H461 IEGRMD Human IgG1 (Pro100-Lys330) N-terminus C-terminus

source short :

CHO

USD :

498 USD

alt names :

frizzled (Drosophila) homolog 8, frizzled 8, frizzled family receptor 8, frizzled homolog 8 (Drosophila), frizzled-8, Frizzled8, FZ-8, FZD8, hFz8

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage