Recombinant Human Frizzled-4 Protein, CF | R&D Systems 5847-FZ-050 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Frizzled-4 Protein, CF

catalog :

5847-FZ-050

quantity :

50 ug

price :

432 USD

more info or order :

R&D Systems product webpage

citations: 2

Reference
Chidiac R, Abedin M, Macleod G, Yang A, Thibeault P, Blazer L, et al. A Norrin/Wnt surrogate antibody stimulates endothelial cell barrier function and rescues retinopathy. EMBO Mol Med. 2021;13:e13977 pubmed publisher
Wilson T, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, et al. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature. 2012;487:505-9 pubmed publisher

product information

master code :

5847-FZ

SKU :

5847-FZ-050

product name :

Recombinant Human Frizzled-4 Protein, CF

unit size :

50 ug

description :

The Recombinant Human Frizzled-4 Protein, CF from R&D Systems is derived from NS0. The Recombinant Human Frizzled-4 Protein, CF has been validated for the following applications: Bioactivity.

target :

Frizzled-4

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

species :

Human

observed molecular weight :

58-60 kDa, reducing conditions

theoretical molecular weight :

42.8 kDa (monomer)

gene symbol :

FZD4

details of functionality :

Measured by its binding ability in a functional ELISA. In a 100 ╡L reaction mixture containing biotinylated rmWnt-5a at 100 ng/mL and rhFrizzled-4/Fc Chimera dilutions at 0.1-2,000 ng/mL, the concentration of rhFrizzled-4/Fc Chimera that produces 50% of the optimal binding response is found to be approximately 15-60 ng/mL. Optimal concentrations should be determined by each laboratory for each application.

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

Q9ULV1

applications :

Bioactivity

source long :

Mouse myeloma cell line, NS0-derived human Frizzled-4 protein Human Frizzled-4 (Phe37-Glu180) Accession # Q9ULV1 IEGRMD Human IgG1 (Pro100-Lys330) N-terminus C-terminus

source short :

NS0

USD :

432 USD

alt names :

CD344, CD344 antigen, EVR1, exudative vitreoretinopathy 1, FEVR, frizzled (Drosophila) homolog 4, frizzled homolog 4 (Drosophila), frizzled-4, Frizzled4, Fz-4, FZD4, FZD4S, FzE4, GPCR, hFz4, MGC34390, WNT receptor frizzled-4

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage