Recombinant Mouse Neurocan Protein, CF | R&D Systems 5800-NC-050 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Mouse Neurocan Protein, CF

catalog :

5800-NC-050

quantity :

50 ug

price :

524 USD

more info or order :

R&D Systems product webpage

citations: 4

Reference
Nakajima C, Sawada M, Umeda E, Takagi Y, Nakashima N, Kuboyama K, et al. Identification of the growth cone as a probe and driver of neuronal migration in the injured brain. Nat Commun. 2024;15:1877 pubmed publisher
Fontanil T, Mohamedi Y, Moncada Pazos A, Cobo T, Vega J, Cobo J, et al. Neurocan is a New Substrate for the ADAMTS12 Metalloprotease: Potential Implications in Neuropathies. Cell Physiol Biochem. 2019;52:1003-1016 pubmed publisher
Sullivan C, Gotthard I, Wyatt E, Bongu S, Mohan V, Weinberg R, et al. Perineuronal Net Protein Neurocan Inhibits NCAM/EphA3 Repellent Signaling in GABAergic Interneurons. Sci Rep. 2018;8:6143 pubmed publisher
Sengottuvel V, Leibinger M, Pfreimer M, Andreadaki A, Fischer D. Taxol facilitates axon regeneration in the mature CNS. J Neurosci. 2011;31:2688-99 pubmed publisher

product information

master code :

5800-NC

SKU :

5800-NC-050

product name :

Recombinant Mouse Neurocan Protein, CF

unit size :

50 ug

description :

The Recombinant Mouse Neurocan Protein, CF from R&D Systems is derived from CHO. The Recombinant Mouse Neurocan Protein, CF has been validated for the following applications: Bioactivity.

target :

Neurocan

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

species :

Mouse

observed molecular weight :

120-200 kDa, reducing conditions

theoretical molecular weight :

67.1 kDa

gene symbol :

Ncan

details of functionality :

Measured by the ability of the immobilized protein to enhance the adhesion of H4 human neuroglioma cells. When 5 x 104 cells/well are added to mouse Neurocan coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is 0.1-0.4 ╡g/mL.

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

P55066

applications :

Bioactivity

source long :

Chinese Hamster Ovary cell line, CHO-derived mouse Neurocan protein Asp23-Asp637, with a C-terminal 10-His tag

source short :

CHO

USD :

524 USD

alt names :

Chondroitin sulfate proteoglycan 3, chondroitin sulfate proteoglycan 3 (neurocan), CSPG3, CSPG3neurocan core protein, FLJ44681, NCAN, NEUR, neurocan, neurocan proteoglycan

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage