Recombinant Human 5'-Nucleotidase/CD73 Protein, CF | R&D Systems 5795-EN-010 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human 5'-Nucleotidase/CD73 Protein, CF

catalog :

5795-EN-010

quantity :

10 ug (also 500 ug)

price :

443 USD

more info or order :

R&D Systems product webpage

citations: 6

Reference
Hong C, Menshikova E, Whiteside T, Jackson E. Assessment of ATP Metabolism to Adenosine by Ecto-Nucleotidases Carried by Tumor-Derived Small Extracellular Vesicles. Res Sq. 2024;: pubmed publisher
Jacoberger Foissac C, Cousineau I, Bareche Y, Allard D, Chrobak P, Allard B, et al. CD73 Inhibits cGAS-STING and Cooperates with CD39 to Promote Pancreatic Cancer. Cancer Immunol Res. 2023;11:56-71 pubmed publisher
Pervan Steel C, Roy Chowdhury U, Sookdeo H, Casale R, Dosa P, Htoo T, et al. Ocular Hypotensive Properties and Biochemical Profile of QLS-101, a Novel ATP-Sensitive Potassium (KATP) Channel Opening Prodrug. Invest Ophthalmol Vis Sci. 2022;63:26 pubmed publisher
Liu S, Li D, Liu J, Wang H, Horecny I, Shen R, et al. A Novel CD73 Inhibitor SHR170008 Suppresses Adenosine in Tumor and Enhances Anti-Tumor Activity with PD-1 Blockade in a Mouse Model of Breast Cancer. Onco Targets Ther. 2021;14:4561-4574 pubmed publisher
Goueli S, Hsiao K. Monitoring and characterizing soluble and membrane-bound ectonucleotidases CD73 and CD39. PLoS ONE. 2019;14:e0220094 pubmed publisher
Yang X, Pei S, Wang H, Jin Y, Yu F, Zhou B, et al. Tiamulin inhibits breast cancer growth and pulmonary metastasis by decreasing the activity of CD73. BMC Cancer. 2017;17:255 pubmed publisher

product information

master code :

5795-EN

SKU :

5795-EN-010

product name :

Recombinant Human 5'-Nucleotidase/CD73 Protein, CF

unit size :

10 ug (also 500 ug)

description :

The Recombinant Human 5'-Nucleotidase/CD73 Protein, CF from R&D Systems is derived from CHO. The Recombinant Human 5'-Nucleotidase/CD73 Protein, CF has been validated for the following applications: Enzyme Activity.

target :

5'-Nucleotidase/CD73

category :

Proteins and Enzymes

buffer :

Supplied as a 0.2 ╡m filtered solution in Tris, NaCl, CaCl2 and Glycerol.

conjugate :

Unconjugated

purity :

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

species :

Human

observed molecular weight :

61-62 kDa, reducing conditions

theoretical molecular weight :

59 kDa

gene symbol :

NT5E

details of functionality :

Measured by its ability to hydrolyze the 5Æ-phosphate group from the substrate adenosine-5Æ-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996 ). The specific activity is 15,000 pmol/min/╡g, as measured under the described conditions.

endotoxin note :

<1.0 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

AAH65937

applications :

Enzyme Activity

source long :

Chinese Hamster Ovary cell line, CHO-derived human 5'-Nucleotidase/CD73 protein Trp27-Lys547, with a C-terminal 6-His tag, Trp27-Lys547

source short :

CHO

USD :

443 USD

alt names :

5' nucleotidase (CD73), 5'-NT, 5-NT, 5'-nucleotidase, ecto (CD73), CD73, CD73 antigen, E5NT, EC 3.1.3.5, ecto-5'-nucleotidase, eN, eNT, NT, NT55'-nucleotidase, NT5E, NTE, Purine 5-Prime-Nucleotidase

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 ░C as supplied. 3 months, -70 ░C under sterile conditions after opening.

more info or order :

R&D Systems product webpage