product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant Mouse E-Selectin/CD62E Fc Chimera Protein, CF
catalog :
575-ES-100
quantity :
100 ug
price :
432 USD
more info or order :
R&D Systems product webpage
citations: 45
Reference
Lien T, Sun D, Chang H. Targeted Delivery to Dying Cells Through P-Selectin-PSGL-1 Axis: A Promising Strategy for Enhanced Drug Efficacy in Liver Injury Models. Cells. 2024;13: pubmed publisher
Pruenster M, Immler R, Roth J, Kuchler T, Bromberger T, Napoli M, et al. E-selectin-mediated rapid NLRP3 inflammasome activation regulates S100A8/S100A9 release from neutrophils via transient gasdermin D pore formation. Nat Immunol. 2023;24:2021-2031 pubmed publisher
Qi J, Jin F, You Y, Du Y, Liu D, Xu X, et al. Synergistic effect of tumor chemo-immunotherapy induced by leukocyte-hitchhiking thermal-sensitive micelles. Nat Commun. 2021;12:4755 pubmed publisher
Anastasiou M, Newton G, Kaur K, Carrillo Salinas F, Smolgovsky S, Bayer A, et al. Endothelial STING controls T cell transmigration in an IFNI-dependent manner. JCI Insight. 2021;6: pubmed publisher
Florez M, Matatall K, Jeong Y, Ortinau L, Shafer P, Lynch A, et al. Interferon Gamma Mediates Hematopoietic Stem Cell Activation and Niche Relocalization through BST2. Cell Rep. 2020;33:108530 pubmed publisher
Azcutia V, Kelm M, Luissint A, Boerner K, Flemming S, Quirós M, et al. Neutrophil expressed CD47 regulates CD11b/CD18-dependent neutrophil transepithelial migration in the intestine in vivo. Mucosal Immunol. 2020;: pubmed publisher
Barbier V, Erbani J, Fiveash C, Davies J, Tay J, Tallack M, et al. Endothelial E-selectin inhibition improves acute myeloid leukaemia therapy by disrupting vascular niche-mediated chemoresistance. Nat Commun. 2020;11:2042 pubmed publisher
Martens R, Permanyer M, Werth K, Yu K, Braun A, Halle O, et al. Efficient homing of T cells via afferent lymphatics requires mechanical arrest and integrin-supported chemokine guidance. Nat Commun. 2020;11:1114 pubmed publisher
Aleisa F, Sakashita K, Lee J, AbuSamra D, Al Alwan B, Nozue S, et al. Functional binding of E-selectin to its ligands is enhanced by structural features beyond its lectin domain. J Biol Chem. 2020;295:3719-3733 pubmed publisher
Hobbs S, Nolz J. Targeted Expansion of Tissue-Resident CD8+ T Cells to Boost Cellular Immunity in the Skin. Cell Rep. 2019;29:2990-2997.e2 pubmed publisher
Mondal N, Silva M, Castano A, Maus M, Sackstein R. Glycoengineering of chimeric antigen receptor (CAR) T-cells to enforce E-selectin binding. J Biol Chem. 2019;294:18465-18474 pubmed publisher
Martin E, Malgor R, Resto V, Goetz D, Burdick M. Dynamic biochemical tissue analysis detects functional selectin ligands on human cancer tissues. Sci Rep. 2019;9:8511 pubmed publisher
Godavarthy P, Kumar R, Herkt S, Pereira R, Hayduk N, Weissenberger E, et al. The vascular bone marrow niche influences outcome in chronic myeloid leukemia via the E-selectin - SCL/TAL1 - CD44 axis. Haematologica. 2019;: pubmed publisher
Carrascal M, Talina C, Borralho P, Gonçalo Mineiro A, Henriques A, Pen C, et al. Staining of E-selectin ligands on paraffin-embedded sections of tumor tissue. BMC Cancer. 2018;18:495 pubmed publisher
Dipiazza A, Laniewski N, Rattan A, Topham D, Miller J, Sant A. CD4 T Cell Epitope Specificity and Cytokine Potential Are Preserved as Cells Transition from the Lung Vasculature to Lung Tissue following Influenza Virus Infection. J Virol. 2018;92: pubmed publisher
Morita K, Tokushige C, Maeda S, Kiyose H, Noura M, Iwai A, et al. RUNX transcription factors potentially control E-selectin expression in the bone marrow vascular niche in mice. Blood Adv. 2018;2:509-515 pubmed publisher
Donnelly C, Dykstra B, Mondal N, Huang J, Kaskow B, Griffin R, et al. Optimizing human Treg immunotherapy by Treg subset selection and E-selectin ligand expression. Sci Rep. 2018;8:420 pubmed publisher
Gittens B, Bodkin J, Nourshargh S, Perretti M, Cooper D. Galectin-3: A Positive Regulator of Leukocyte Recruitment in the Inflamed Microcirculation. J Immunol. 2017;198:4458-4469 pubmed publisher
Pan Y, Tian T, Park C, Lofftus S, Mei S, Liu X, et al. Survival of tissue-resident memory T cells requires exogenous lipid uptake and metabolism. Nature. 2017;543:252-256 pubmed publisher
Jiang X, Park C, Geddes Sweeney J, Yoo M, Gaide O, Kupper T. Dermal ?? T Cells Do Not Freely Re-Circulate Out of Skin and Produce IL-17 to Promote Neutrophil Infiltration during Primary Contact Hypersensitivity. PLoS ONE. 2017;12:e0169397 pubmed publisher
Buffone A, Nasirikenari M, Manhardt C, Lugade A, Bogner P, Sackstein R, et al. Leukocyte-borne ?(1,3)-fucose is a negative regulator of ?2-integrin-dependent recruitment in lung inflammation. J Leukoc Biol. 2017;101:459-470 pubmed publisher
Csépányi Kömi R, Wisniewski E, Bartos B, Lévai P, Nemeth T, Balazs B, et al. Rac GTPase Activating Protein ARHGAP25 Regulates Leukocyte Transendothelial Migration in Mice. J Immunol. 2016;197:2807-15 pubmed publisher
Velázquez F, Grodecki Pena A, Knapp A, Salvador A, NEVERS T, Croce K, et al. CD43 Functions as an E-Selectin Ligand for Th17 Cells In Vitro and Is Required for Rolling on the Vascular Endothelium and Th17 Cell Recruitment during Inflammation In Vivo. J Immunol. 2016;196:1305-1316 pubmed publisher
Krishnamurthy V, Sardar M, Ying Y, Song X, Haller C, Dai E, et al. Glycopeptide analogues of PSGL-1 inhibit P-selectin in vitro and in vivo. Nat Commun. 2015;6:6387 pubmed publisher
Abdi R, Moore R, Sakai S, Donnelly C, Mounayar M, Sackstein R. HCELL Expression on Murine MSC Licenses Pancreatotropism and Confers Durable Reversal of Autoimmune Diabetes in NOD Mice. Stem Cells. 2015;33:1523-31 pubmed publisher
Germena G, Volmering S, Sohlbach C, Zarbock A. Mutation in the CD45 inhibitory wedge modulates integrin activation and leukocyte recruitment during inflammation. J Immunol. 2015;194:728-38 pubmed publisher
Woollard K, Lumsden N, Andrews K, Aprico A, Harris E, Irvine J, et al. Raised soluble P-selectin moderately accelerates atherosclerotic plaque progression. PLoS ONE. 2014;9:e97422 pubmed publisher
Sato S, Sakurai T, Ogasawara J, Takahashi M, Izawa T, Imaizumi K, et al. A circadian clock gene, Rev-erb?, modulates the inflammatory function of macrophages through the negative regulation of Ccl2 expression. J Immunol. 2014;192:407-17 pubmed publisher
Li J, Guillebon A, Hsu J, Barthel S, Dimitroff C, Lee Y, et al. Human fucosyltransferase 6 enables prostate cancer metastasis to bone. Br J Cancer. 2013;109:3014-22 pubmed publisher
Giegold O, Ogrissek N, Richter C, Schröder M, Herrero San Juan M, Pfeilschifter J, et al. CXCL9 causes heterologous desensitization of CXCL12-mediated memory T lymphocyte activation. J Immunol. 2013;190:3696-705 pubmed publisher
Alcaide P, Maganto Garcia E, Newton G, Travers R, CROCE K, Bu D, et al. Difference in Th1 and Th17 lymphocyte adhesion to endothelium. J Immunol. 2012;188:1421-30 pubmed publisher
Takeuchi H, Yokota A, Ohoka Y, Kagechika H, Kato C, Song S, et al. Efficient induction of CCR9 on T cells requires coactivation of retinoic acid receptors and retinoid X receptors (RXRs): exaggerated T Cell homing to the intestine by RXR activation with organotins. J Immunol. 2010;185:5289-99 pubmed publisher
Kato N, Yuzawa Y, Kosugi T, Hobo A, Sato W, Miwa Y, et al. The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion. J Am Soc Nephrol. 2009;20:1565-76 pubmed publisher
Sinclair L, Finlay D, Feijoo C, Cornish G, Gray A, Ager A, et al. Phosphatidylinositol-3-OH kinase and nutrient-sensing mTOR pathways control T lymphocyte trafficking. Nat Immunol. 2008;9:513-21 pubmed publisher
Yamanaka K, Dimitroff C, Fuhlbrigge R, Kakeda M, Kurokawa I, Mizutani H, et al. Vitamins A and D are potent inhibitors of cutaneous lymphocyte-associated antigen expression. J Allergy Clin Immunol. 2008;121:148-157.e3 pubmed
Raes G, Ghassabeh G, Brys L, Mpofu N, Verschueren H, Vanhecke D, et al. The metastatic T-cell hybridoma antigen/P-selectin glycoprotein ligand 1 is required for hematogenous metastasis of lymphomas. Int J Cancer. 2007;121:2646-52 pubmed
Alcaide P, King S, Dimitroff C, Lim Y, Fuhlbrigge R, Luscinskas F. The 130-kDa glycoform of CD43 functions as an E-selectin ligand for activated Th1 cells in vitro and in delayed-type hypersensitivity reactions in vivo. J Invest Dermatol. 2007;127:1964-72 pubmed
Ni Z, Walcheck B. Varied levels of reactivity by different E-selectin/Fc constructs with cutaneous lymphocyte-associated antigen (CLA)(+) CD4(+) T cells. Immunol Lett. 2007;108:179-82 pubmed
Monzavi Karbassi B, Stanley J, Hennings L, Jousheghany F, Artaud C, Shaaf S, et al. Chondroitin sulfate glycosaminoglycans as major P-selectin ligands on metastatic breast cancer cell lines. Int J Cancer. 2007;120:1179-91 pubmed
Krause D, Lazarides K, von Andrian U, Van Etten R. Requirement for CD44 in homing and engraftment of BCR-ABL-expressing leukemic stem cells. Nat Med. 2006;12:1175-80 pubmed
Ni Z, Campbell J, Niehans G, Walcheck B. The monoclonal antibody CHO-131 identifies a subset of cutaneous lymphocyte-associated antigen T cells enriched in P-selectin-binding cells. J Immunol. 2006;177:4742-8 pubmed
Liu L, Fuhlbrigge R, Karibian K, Tian T, Kupper T. Dynamic programming of CD8+ T cell trafficking after live viral immunization. Immunity. 2006;25:511-20 pubmed
Zanardo R, Bonder C, Hwang J, Andonegui G, Liu L, Vestweber D, et al. A down-regulatable E-selectin ligand is functionally important for PSGL-1-independent leukocyte-endothelial cell interactions. Blood. 2004;104:3766-73 pubmed
Dudda J, Simon J, Martin S. Dendritic cell immunization route determines CD8+ T cell trafficking to inflamed skin: role for tissue microenvironment and dendritic cells in establishment of T cell-homing subsets. J Immunol. 2004;172:857-63 pubmed
Cardones A, Murakami T, Hwang S. CXCR4 enhances adhesion of B16 tumor cells to endothelial cells in vitro and in vivo via beta(1) integrin. Cancer Res. 2003;63:6751-7 pubmed
product information
brand :
R&D Systems
master code :
575-ES
SKU :
575-ES-100
product name :
Recombinant Mouse E-Selectin/CD62E Fc Chimera Protein, CF
unit size :
100 ug
seo description :
The Recombinant Mouse E-Selectin/CD62E Fc Chimera Protein, CF from R&D Systems is derived from NS0. The Recombinant Mouse E-Selectin/CD62E Fc Chimera Protein, CF has been validated for the following applications: Bioactivity.
target :
E-Selectin/CD62E
category :
Proteins and Enzymes
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS.
conjugate :
Unconjugated
dilution :
Bioactivity
purity :
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.
species :
Mouse
observed molecular weight :
110 - 130 kDa, reducing conditions
theoretical molecular weight :
85.8 kDa (monomer)
gene symbol :
Sele
details of functionality :
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 104 cells/well are added to mouse E-Selectin/Fc Chimera coated plates (2 ╡g/mL with 100 ╡L/well), approximately 85-100% will adhere for 1 hour incubation at room temperature. Optimal dilutions should be determined by each laboratory for each application.
endotoxin note :
<1.0 EU per 1 ╡g of the protein by the LAL method.
accessionNumbers :
Q00690
applications :
Bioactivity
source :
NS0-derived Recombinant Mouse E-Selectin/CD62E Fc Chimera Protein, CF
USD :
432 USD
alt names :
CD62 antigen-like family member E, CD62E, CD62E antigen, ELAM, ELAM1, ELAM-1, ELAM1E-selectin, endothelial adhesion molecule 1, Endothelial leukocyte adhesion molecule 1, ESEL, LECAM2, leukocyte endothelial cell adhesion molecule 2, Leukocyte-endothelial cell adhesion molecule 2, SELE, selectin E
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.