Recombinant Human Integrin alpha 4 beta 1 Protein, CF | R&D Systems 5668-A4-050 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Integrin alpha 4 beta 1 Protein, CF

catalog :

5668-A4-050

quantity :

50 ug

price :

491 USD

more info or order :

R&D Systems product webpage

citations: 7

Reference
Naoi Y, Morinaga T, Nagasaki J, Ariyasu R, Ueda Y, Yamashita K, et al. CD106 in Tumor-Specific Exhausted CD8+ T Cells Mediates Immunosuppression by Inhibiting TCR Signaling. Cancer Res. 2024;84:2109-2122 pubmed publisher
Cegarra C, Cameron B, Chaves C, Dabdoubi T, Do T, Gen xea t B, et al. An innovative strategy to identify new targets for delivering antibodies to the brain has led to the exploration of the integrin family. PLoS ONE. 2022;17:e0274667 pubmed publisher
Fnu G, Agrawal P, Kundu G, Weber G. Structural Constraint of Osteopontin Facilitates Efficient Binding to CD44. Biomolecules. 2021;11: pubmed publisher
Lertjuthaporn S, Cicala C, Van Ryk D, Liu M, Yolitz J, Wei D, et al. Select gp120 V2 domain specific antibodies derived from HIV and SIV infection and vaccination inhibit gp120 binding to α4β7. PLoS Pathog. 2018;14:e1007278 pubmed publisher
Skoog E, Morikis V, Martin M, Foster G, Cai L, Hansen L, et al. CagY-Dependent Regulation of Type IV Secretion in Helicobacter pylori Is Associated with Alterations in Integrin Binding. MBio. 2018;9: pubmed publisher
Chakraborty S, Hu S, Wu S, Karmenyan A, Chiou A. The interaction affinity between vascular cell adhesion molecule-1 (VCAM-1) and very late antigen-4 (VLA-4) analyzed by quantitative FRET. PLoS ONE. 2015;10:e0121399 pubmed publisher
Azab W, Zajic L, Osterrieder N. The role of glycoprotein H of equine herpesviruses 1 and 4 (EHV-1 and EHV-4) in cellular host range and integrin binding. Vet Res. 2012;43:61 pubmed publisher

product information

master code :

5668-A4

SKU :

5668-A4-050

product name :

Recombinant Human Integrin alpha 4 beta 1 Protein, CF

unit size :

50 ug

description :

The Recombinant Human Integrin alpha 4 beta 1 Protein, CF from R&D Systems is derived from CHO. The Recombinant Human Integrin alpha 4 beta 1 Protein, CF has been validated for the following applications: Bioactivity.

target :

Integrin alpha 4 beta 1

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS.

conjugate :

Unconjugated

purity :

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Human

observed molecular weight :

100-150 kDa, reducing conditions

theoretical molecular weight :

108.8 kDa ( alpha 4) 82.3 kDa (▀1)

gene symbol :

ITGA4

details of functionality :

Measured by the ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with VCAM-1. When 5 x 104 cells per well are added to rhIntegrin alpha 4▀1 coated plates (10 ╡g/mL, 100 ╡L/well), approximately 45%-75% will adhere after 1 hour at 37 ░C.

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

P13612 (Integrin alpha 4) P05556 (Integrin ▀1)

applications :

Bioactivity

source long :

Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha 4 beta 1 protein Human Integrin alpha 4 (Tyr34-Gln970 (Arg591Leu Arg878Gln)) Accession # P13612 Acidic Tail 6-His tag Human Integrin ▀1 (Gln21-Asp728) Accession # P05556 Basic Tail N-terminus C-terminus

source short :

CHO

USD :

491 USD

alt names :

CD49d, IA4

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage