Recombinant Rat GFR alpha-1 Fc Chimera Protein | R&D Systems 560-GR-100 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Rat GFR alpha-1 Fc Chimera Protein

catalog :

560-GR-100

quantity :

100 ug

price :

432 USD

citations: 16

Reference
Song Y, Zhang X, Desmarais J, Nagano M. Postnatal development of mouse spermatogonial stem cells as determined by immunophenotype, regenerative capacity, and long-term culture-initiating ability: a model for practical applications. Sci Rep. 2024;14:2299 pubmed publisher
Rajachandran S, Zhang X, Cao Q, Caldeira Brant A, Zhang X, Song Y, et al. Dissecting the spermatogonial stem cell niche using spatial transcriptomics. Cell Rep. 2023;42:112737 pubmed publisher
Boyer A, Zhang X, Levasseur A, Abou Nader N, St Jean G, Nagano M, et al. Constitutive activation of CTNNB1 results in a loss of spermatogonial stem cell activity in mice. PLoS ONE. 2021;16:e0251911 pubmed publisher
Porokuokka L, Virtanen H, Lindén J, Sidorova Y, Danilova T, Lindahl M, et al. Gfra1 Underexpression Causes Hirschsprung's Disease and Associated Enterocolitis in Mice. Cell Mol Gastroenterol Hepatol. 2019;7:655-678 pubmed publisher
Shindo T, Doi S, Nakashima A, Sasaki K, Arihiro K, Masaki T. TGF-?1 promotes expression of fibrosis-related genes through the induction of histone variant H3.3 and histone chaperone HIRA. Sci Rep. 2018;8:14060 pubmed publisher
Gassei K, Sheng Y, Fayomi A, Mital P, Sukhwani M, Lin C, et al. DDX4-EGFP transgenic rat model for the study of germline development and spermatogenesis. Biol Reprod. 2017;96:707-719 pubmed publisher
Yuan J, Liu W, Zhu H, Chen Y, Zhang X, Li L, et al. Curcumin inhibits glial scar formation by suppressing astrocyte-induced inflammation and fibrosis in vitro and in vivo. Brain Res. 2017;1655:90-103 pubmed publisher
Ibiza S, García Cassani B, Ribeiro H, Carvalho T, Almeida L, Marques R, et al. Glial-cell-derived neuroregulators control type 3 innate lymphoid cells and gut defence. Nature. 2016;535:440-443 pubmed publisher
Fonseca Pereira D, Arroz Madeira S, Rodrigues Campos M, Barbosa I, Domingues R, Bento T, et al. The neurotrophic factor receptor RET drives haematopoietic stem cell survival and function. Nature. 2014;514:98-101 pubmed publisher
Sakuraba J, Shintani T, Tani S, Noda M. Substrate specificity of R3 receptor-like protein-tyrosine phosphatase subfamily toward receptor protein-tyrosine kinases. J Biol Chem. 2013;288:23421-31 pubmed publisher
Euteneuer S, Yang K, Chavez E, Leichtle A, Loers G, Olshansky A, et al. Glial cell line-derived neurotrophic factor (GDNF) induces neuritogenesis in the cochlear spiral ganglion via neural cell adhesion molecule (NCAM). Mol Cell Neurosci. 2013;54:30-43 pubmed publisher
Kubota H, Wu X, Goodyear S, Avarbock M, Brinster R. Glial cell line-derived neurotrophic factor and endothelial cells promote self-renewal of rabbit germ cells with spermatogonial stem cell properties. FASEB J. 2011;25:2604-14 pubmed publisher
Carmillo P, Dagø L, Day E, Worley D, Rossomando A, Walus L, et al. Glial cell line-derived neurotrophic factor (GDNF) receptor alpha-1 (GFR alpha 1) is highly selective for GDNF versus artemin. Biochemistry. 2005;44:2545-54 pubmed
Schuetz G, Rosario M, Grimm J, Boeckers T, Gundelfinger E, Birchmeier W. The neuronal scaffold protein Shank3 mediates signaling and biological function of the receptor tyrosine kinase Ret in epithelial cells. J Cell Biol. 2004;167:945-52 pubmed
Cerchia L, Libri D, Carlomagno M, de Franciscis V. The soluble ectodomain of RetC634Y inhibits both the wild-type and the constitutively active Ret. Biochem J. 2003;372:897-903 pubmed
Peterziel H, Unsicker K, Krieglstein K. TGFbeta induces GDNF responsiveness in neurons by recruitment of GFRalpha1 to the plasma membrane. J Cell Biol. 2002;159:157-67 pubmed

product information

master code :

560-GR

SKU :

560-GR-100

product name :

Recombinant Rat GFR alpha-1 Fc Chimera Protein

unit size :

100 ug

description :

The Recombinant Rat GFR alpha-1 Fc Chimera Protein from R&D Systems is derived from NS0. The Recombinant Rat GFR alpha-1 Fc Chimera Protein has been validated for the following applications: Bioactivity, Binding Activity.

target :

GFR alpha-1/GDNF R alpha-1

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

species :

Rat

observed molecular weight :

95 kDa, reducing conditions

theoretical molecular weight :

74 kDa (monomer)

gene symbol :

Gfra1

details of functionality :

Measured by its ability to confer GDNF responsiveness to PC-12 rat adrenal pheochromocytoma cells using a neurite outgrowth assay. Treanor, J. et al . (1996) Nature 392 :80. The ED50 for this effect is approximately 0.3 ╡g/mL in the presence of 25 ng/mL of Recombinant Rat GDNF (Catalog # 512-GF ). Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rat GDNF (Catalog # 512-GF ) at 1 ╡g/mL binds Recombinant Rat GFR alpha -1/GDNF R alpha -1 Fc Chimera with an apparent KD 5 nM.

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

Q62997

applications :

Bioactivity, Binding Activity

source long :

Mouse myeloma cell line, NS0-derived rat GFR alpha-1/GDNF R alpha-1 protein Rat GFR alpha -1 (Asp25-Leu445) Accession # Q62997 CSIEGRMD Human IgG1 (Pro100-Lys330) 6-His tag N-terminus C-terminus

source short :

NS0

USD :

432 USD

alt names :

DKFZp313E1012, DKFZp686J0156, GDNF family receptor alpha 1, GDNF family receptor alpha-1, GDNF R alpha-1, GDNF receptor alpha-1, GDNFRAGFR-ALPHA-1, GDNFR-alpha-1, GDNFRMGC23045, GFR alpha1, GFRa-1, GFRA1, GFR-alpha-1, Glial cell line-derived neurotrophic factor receptor alpha, GPI-linked anchor protein, PI-linked cell-surface accessory protein, RET ligand 1, RET1L, RETL1FLJ10561, TGF-beta related neurotrophic factor receptor 1, TGF-beta-related neurotrophic factor receptor 1, TRNR1FLJ31546

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.