Recombinant Rat CNTF Protein | R&D Systems 557-NT-010 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Rat CNTF Protein

catalog :

557-NT-010

quantity :

10 ug (also 50 ug)

price :

211 USD

more info or order :

R&D Systems product webpage

citations: 36

Reference
Cohn E, Clayton B, Madhavan M, Yacoub S, Federov Y, Paul Friedman K, et al. Pervasive environmental chemicals impair oligodendrocyte development. bioRxiv. 2023;: pubmed publisher
Tang Y, Kwiatkowski D, Henske E. Midkine expression by stem-like tumor cells drives persistence to mTOR inhibition and an immune-suppressive microenvironment. Nat Commun. 2022;13:5018 pubmed publisher
Martin S, Allan K, Pinkard O, Sweet T, Tesar P, Coller J. Oligodendrocyte differentiation alters tRNA modifications and codon optimality-mediated mRNA decay. Nat Commun. 2022;13:5003 pubmed publisher
D Este G, Stazi M, Negro S, Megighian A, Lista F, Rossetto O, et al. Latrotoxin-Induced Neuromuscular Junction Degeneration Reveals Urocortin 2 as a Critical Contributor to Motor Axon Terminal Regeneration. Int J Mol Sci. 2022;23: pubmed publisher
Allan K, Hu L, Scavuzzo M, Morton A, Gevorgyan A, Cohn E, et al. Non-canonical Targets of HIF1a Impair Oligodendrocyte Progenitor Cell Function. Cell Stem Cell. 2021;28:257-272.e11 pubmed publisher
Collura K, Niu J, Sanders S, Montersino A, Holland S, Thomas G. The palmitoyl acyltransferases ZDHHC5 and ZDHHC8 are uniquely present in DRG axons and control retrograde signaling via the Gp130/JAK/STAT3 pathway. J Biol Chem. 2020;295:15427-15437 pubmed publisher
Bucher F, Aguilar E, Marra K, Rapp J, Arnold J, Diaz Aguilar S, et al. CNTF Prevents Development of Outer Retinal Neovascularization Through Upregulation of CxCl10. Invest Ophthalmol Vis Sci. 2020;61:20 pubmed publisher
Soulard C, Salsac C, Mouzat K, Hilaire C, Roussel J, Mezghrani A, et al. Spinal Motoneuron TMEM16F Acts at C-boutons to Modulate Motor Resistance and Contributes to ALS Pathogenesis. Cell Rep. 2020;30:2581-2593.e7 pubmed publisher
Munezane H, Oizumi H, Wakabayashi T, Nishio S, Hirasawa T, Sato T, et al. Roles of Collagen XXV and Its Putative Receptors PTPσ/δ in Intramuscular Motor Innervation and Congenital Cranial Dysinnervation Disorder. Cell Rep. 2019;29:4362-4376.e6 pubmed publisher
Lindahl H, Guerreiro Cacais A, Bedri S, Linnerbauer M, Lindén M, Abdelmagid N, et al. IL-22 Binding Protein Promotes the Disease Process in Multiple Sclerosis. J Immunol. 2019;203:888-898 pubmed publisher
Funk N, Munz M, Ott T, Brockmann K, Wenninger Weinzierl A, Kühn R, et al. The Parkinson's disease-linked Leucine-rich repeat kinase 2 (LRRK2) is required for insulin-stimulated translocation of GLUT4. Sci Rep. 2019;9:4515 pubmed publisher
Wu C, Watts M, Rubin L. MAP4K4 Activation Mediates Motor Neuron Degeneration in Amyotrophic Lateral Sclerosis. Cell Rep. 2019;26:1143-1156.e5 pubmed publisher
Di Pierdomenico J, Scholz R, Valiente Soriano F, Sanchez Migallon M, Vidal Sanz M, Langmann T, et al. Neuroprotective Effects of FGF2 and Minocycline in Two Animal Models of Inherited Retinal Degeneration. Invest Ophthalmol Vis Sci. 2018;59:4392-4403 pubmed publisher
Gibbs K, Kalmar B, Rhymes E, Fellows A, Ahmed M, Whiting P, et al. Inhibiting p38 MAPK alpha rescues axonal retrograde transport defects in a mouse model of ALS. Cell Death Dis. 2018;9:596 pubmed publisher
Rajagopal R, Zhang S, Wei X, Doggett T, Adak S, Enright J, et al. Retinal de novo lipogenesis coordinates neurotrophic signaling to maintain vision. JCI Insight. 2018;3: pubmed publisher
Jenkinson S, Grandgirard D, Heidemann M, Tscherter A, Avondet M, Leib S. Embryonic Stem Cell-Derived Neurons Grown on Multi-Electrode Arrays as a Novel In vitro Bioassay for the Detection of Clostridium botulinum Neurotoxins. Front Pharmacol. 2017;8:73 pubmed publisher
Schaller S, Buttigieg D, Alory A, Jacquier A, Barad M, Merchant M, et al. Novel combinatorial screening identifies neurotrophic factors for selective classes of motor neurons. Proc Natl Acad Sci U S A. 2017;114:E2486-E2493 pubmed publisher
Bucher F, Walz J, Bühler A, Aguilar E, Lange C, Diaz Aguilar S, et al. CNTF Attenuates Vasoproliferative Changes Through Upregulation of SOCS3 in a Mouse-Model of Oxygen-Induced Retinopathy. Invest Ophthalmol Vis Sci. 2016;57:4017-26 pubmed publisher
Vilmont V, Cadot B, Ouanounou G, Gomes E. A system for studying mechanisms of neuromuscular junction development and maintenance. Development. 2016;143:2464-77 pubmed publisher
Schmieg N, Thomas C, Yabe A, Lynch D, Iglesias T, Chakravarty P, et al. Novel Kidins220/ARMS Splice Isoforms: Potential Specific Regulators of Neuronal and Cardiovascular Development. PLoS ONE. 2015;10:e0129944 pubmed publisher
Hong S, Song M. Signal transducer and activator of transcription-3 maintains the stemness of radial glia at mid-neurogenesis. J Neurosci. 2015;35:1011-23 pubmed publisher
Terenzio M, Golding M, Russell M, Wicher K, Rosewell I, Spencer Dene B, et al. Bicaudal-D1 regulates the intracellular sorting and signalling of neurotrophin receptors. EMBO J. 2014;33:1582-98 pubmed publisher
Elsaeidi F, Bemben M, Zhao X, Goldman D. Jak/Stat signaling stimulates zebrafish optic nerve regeneration and overcomes the inhibitory actions of Socs3 and Sfpq. J Neurosci. 2014;34:2632-44 pubmed publisher
Severi I, Perugini J, Mondini E, Smorlesi A, Frontini A, Cinti S, et al. Opposite effects of a high-fat diet and calorie restriction on ciliary neurotrophic factor signaling in the mouse hypothalamus. Front Neurosci. 2013;7:263 pubmed publisher
Dubovy P, Raska O, Klusáková I, Stejskal L, Celakovský P, Haninec P. Ciliary neurotrophic factor promotes motor reinnervation of the musculocutaneous nerve in an experimental model of end-to-side neurorrhaphy. BMC Neurosci. 2011;12:58 pubmed publisher
Krencik R, Weick J, Liu Y, Zhang Z, Zhang S. Specification of transplantable astroglial subtypes from human pluripotent stem cells. Nat Biotechnol. 2011;29:528-34 pubmed publisher
Kent T, Glybina I, Abrams G, Iezzi R. Chronic intravitreous infusion of ciliary neurotrophic factor modulates electrical retinal stimulation thresholds in the RCS rat. Invest Ophthalmol Vis Sci. 2008;49:372-9 pubmed publisher
McGill T, Prusky G, Douglas R, Yasumura D, Matthes M, Nune G, et al. Intraocular CNTF reduces vision in normal rats in a dose-dependent manner. Invest Ophthalmol Vis Sci. 2007;48:5756-66 pubmed
Ko M, Liu Y, Dryer S, Ko G. The expression of L-type voltage-gated calcium channels in retinal photoreceptors is under circadian control. J Neurochem. 2007;103:784-92 pubmed
Nakanishi M, Niidome T, Matsuda S, Akaike A, Kihara T, Sugimoto H. Microglia-derived interleukin-6 and leukaemia inhibitory factor promote astrocytic differentiation of neural stem/progenitor cells. Eur J Neurosci. 2007;25:649-58 pubmed
Janoschek R, Plum L, Koch L, Munzberg H, Diano S, Shanabrough M, et al. gp130 signaling in proopiomelanocortin neurons mediates the acute anorectic response to centrally applied ciliary neurotrophic factor. Proc Natl Acad Sci U S A. 2006;103:10707-12 pubmed
Hapner S, Nielsen K, Chaverra M, Esper R, Loeb J, Lefcort F. NT-3 and CNTF exert dose-dependent, pleiotropic effects on cells in the immature dorsal root ganglion: neuregulin-mediated proliferation of progenitor cells and neuronal differentiation. Dev Biol. 2006;297:182-97 pubmed
Murata T, Tsuboi M, Hikita K, Kaneda N. Protective effects of neurotrophic factors on tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis of murine adrenal chromaffin cell line tsAM5D. J Biol Chem. 2006;281:22503-16 pubmed
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Fischer A, Omar G, Eubanks J, McGuire C, Dierks B, Reh T. Different aspects of gliosis in retinal Muller glia can be induced by CNTF, insulin, and FGF2 in the absence of damage. Mol Vis. 2004;10:973-86 pubmed
Mizisin A, Vu Y, Shuff M, Calcutt N. Ciliary neurotrophic factor improves nerve conduction and ameliorates regeneration deficits in diabetic rats. Diabetes. 2004;53:1807-12 pubmed

product information

brand :

R&D Systems

master code :

557-NT

SKU :

557-NT-010

product name :

Recombinant Rat CNTF Protein

unit size :

10 ug (also 50 ug)

seo description :

The Recombinant Rat CNTF Protein from R&D Systems is derived from E. coli. The Recombinant Rat CNTF Protein has been validated for the following applications: Bioactivity.

target :

CNTF

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in Tris, NaCl, TCEP, EDTA, CHAPS and Trehalose with BSA as a carrier protein.

conjugate :

Unconjugated

dilution :

Bioactivity

purity :

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain

species :

Rat

theoretical molecular weight :

22.8 kDa

gene symbol :

Cntf

details of functionality :

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al . (1989) J. Cell Physiol. 140 :323. The ED50 for this effect is 3-15 ng/mL. Measured by its ability to support the survival and stimulate neurite outgrowth of dissociated chick embryonic dorsal root ganglia (DRG) neurons. Davies,áA.M. (1989) in Neurotrophic Factor Bioassay Using Dissociated Neurons , Nerve Growth Factor. Rush,áR.A. (eds): John Willey and Sons, Ltd. 95. The ED50 for this effect is 0.1-0.3 ng/mL.

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

P20294.1

applications :

Bioactivity

source :

E. coli-derived Recombinant Rat CNTF Protein

USD :

211 USD

alt names :

ciliary neurotrophic factor, HCNTF

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage