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company name :
R&D Systems
product type :
protein
product name :
Recombinant Rat Agrin Protein
catalog :
550-AG-100
quantity :
100 ug
price :
498 USD
more info or order :
citations: 29
Reference
Masingue M, Cattaneo O, Wolff N, Buon C, Sternberg D, Euchparmakian M, et al. New mutation in the β1 propeller domain of LRP4 responsible for congenital myasthenic syndrome associated with Cenani-Lenz syndrome. Sci Rep. 2023;13:14054 pubmed publisher
Burmeister M, Frauenstein A, Kahms M, Arends L, Gerwien H, Deshpande T, et al. Secretomics reveals gelatinase substrates at the blood-brain barrier that are implicated in astroglial barrier function. Sci Adv. 2023;9:eadg0686 pubmed publisher
Uyen Dao T, Barbeau S, Mess xe9 ant J, Della Gaspera B, Bouceba T, Semprez F, et al. The collagen ColQ binds to LRP4 and regulates the activation of the Muscle-Specific Kinase-LRP4 receptor complex by agrin at the neuromuscular junction. J Biol Chem. 2023;299:104962 pubmed publisher
Kwan H, Chan Z, Bi X, Kutkowska J, Pr xf3 szy x144 ski T, Chan C, et al. Nerve-independent formation of membrane infoldings at topologically complex postsynaptic apparatus by caveolin-3. Sci Adv. 2023;9:eadg0183 pubmed publisher
Lim J, Augustinus R, Plomp J, Roya Kouchaki K, Vergoossen D, Filli xe9 Grijpma Y, et al. Development and characterization of agonistic antibodies targeting the Ig-like 1 domain of MuSK. Sci Rep. 2023;13:7478 pubmed publisher
Massih B, Veh A, Schenke M, Mungwa S, Seeger B, Selvaraj B, et al. A 3D cell culture system for bioengineering human neuromuscular junctions to model ALS. Front Cell Dev Biol. 2023;11:996952 pubmed publisher
Gros K, Matkovi x10d U, Parato G, Mi x161 K, Luin E, Bernareggi A, et al. Neuronal Agrin Promotes Proliferation of Primary Human Myoblasts in an Age-Dependent Manner. Int J Mol Sci. 2022;23: pubmed publisher
Klockner I, Schütt C, Gerhardt T, Boettger T, Braun T. Control of CRK-RAC1 activity by the miR-1/206/133 miRNA family is essential for neuromuscular junction function. Nat Commun. 2022;13:3180 pubmed publisher
Shipton O, Tang C, Paulsen O, Vargas Caballero M. Differential vulnerability of hippocampal CA3-CA1 synapses to Aβ. Acta Neuropathol Commun. 2022;10:45 pubmed publisher
Yamamotoya T, Hasei S, Akasaka Y, Ohata Y, Nakatsu Y, Kanna M, et al. Involvement of neuronal and muscular Trk-fused gene (TFG) defects in the development of neurodegenerative diseases. Sci Rep. 2022;12:1966 pubmed publisher
Pinto C, Perez V, Mella J, Albistur M, Caprile T, Bronfman F, et al. Transport and Secretion of the Wnt3 Ligand by Motor Neuron-like Cells and Developing Motor Neurons. Biomolecules. 2021;11: pubmed publisher
Oury J, Zhang W, Leloup N, Koide A, Corrado A, Ketavarapu G, et al. Mechanism of disease and therapeutic rescue of Dok7 congenital myasthenia. Nature. 2021;595:404-408 pubmed publisher
Proietti D, Giordani L, De Bardi M, D Ercole C, Lozanoska Ochser B, Amadio S, et al. Activation of skeletal muscle-resident glial cells upon nerve injury. JCI Insight. 2021;6: pubmed publisher
Oentaryo M, Tse A, Lee C. Neuronal MT1-MMP mediates ECM clearance and Lrp4 cleavage for agrin deposition and signaling in presynaptic development. J Cell Sci. 2020;133: pubmed publisher
Chen P, Zelada D, Belhasan D, Akaaboune M. Phosphorylation of α-dystrobrevin is essential for αkap accumulation and acetylcholine receptor stability. J Biol Chem. 2020;295:10677-10688 pubmed publisher
Xing G, Jing H, Zhang L, Cao Y, Li L, Zhao K, et al. A mechanism in agrin signaling revealed by a prevalent Rapsyn mutation in congenital myasthenic syndrome. elife. 2019;8: pubmed publisher
Njah K, Chakraborty S, Qiu B, Arumugam S, Raju A, Pobbati A, et al. A Role of Agrin in Maintaining the Stability of Vascular Endothelial Growth Factor Receptor-2 during Tumor Angiogenesis. Cell Rep. 2019;28:949-965.e7 pubmed publisher
Takata K, Stathopoulos P, Cao M, Mané Damas M, Fichtner M, Benotti E, et al. Characterization of pathogenic monoclonal autoantibodies derived from muscle-specific kinase myasthenia gravis patients. JCI Insight. 2019;4: pubmed publisher
Blondelle J, Tallapaka K, Seto J, Ghassemian M, Clark M, Laitila J, et al. Cullin-3 dependent deregulation of ACTN1 represents a new pathogenic mechanism in nemaline myopathy. JCI Insight. 2019;5: pubmed publisher
Maffioletti S, Sarcar S, Henderson A, Mannhardt I, Pinton L, Moyle L, et al. Three-Dimensional Human iPSC-Derived Artificial Skeletal Muscles Model Muscular Dystrophies and Enable Multilineage Tissue Engineering. Cell Rep. 2018;23:899-908 pubmed publisher
Cantor S, Zhang W, Delestrée N, Remédio L, Mentis G, Burden S. Preserving neuromuscular synapses in ALS by stimulating MuSK with a therapeutic agonist antibody. elife. 2018;7: pubmed publisher
Ito K, Ohkawara B, Yagi H, Nakashima H, Tsushima M, Ota K, et al. Lack of Fgf18 causes abnormal clustering of motor nerve terminals at the neuromuscular junction with reduced acetylcholine receptor clusters. Sci Rep. 2018;8:434 pubmed publisher
Blondelle J, Shapiro P, Domenighetti A, Lange S. Cullin E3 Ligase Activity Is Required for Myoblast Differentiation. J Mol Biol. 2017;429:1045-1066 pubmed publisher
Nakashima H, Ohkawara B, Ishigaki S, Fukudome T, Ito K, Tsushima M, et al. R-spondin 2 promotes acetylcholine receptor clustering at the neuromuscular junction via Lgr5. Sci Rep. 2016;6:28512 pubmed publisher
Chen Y, Ip F, Shi L, Zhang Z, Tang H, Ng Y, et al. Coronin 6 regulates acetylcholine receptor clustering through modulating receptor anchorage to actin cytoskeleton. J Neurosci. 2014;34:2413-21 pubmed publisher
Ohkawara B, Cabrera Serrano M, Nakata T, Milone M, Asai N, Ito K, et al. LRP4 third β-propeller domain mutations cause novel congenital myasthenia by compromising agrin-mediated MuSK signaling in a position-specific manner. Hum Mol Genet. 2014;23:1856-68 pubmed publisher
Choi H, Liu Y, Tennert C, Sugiura Y, Karakatsani A, Kroger S, et al. APP interacts with LRP4 and agrin to coordinate the development of the neuromuscular junction in mice. elife. 2013;2:e00220 pubmed publisher
Xu X, Fu A, Ip F, Wu C, Duan S, Poo M, et al. Agrin regulates growth cone turning of Xenopus spinal motoneurons. Development. 2005;132:4309-16 pubmed
Finn A, Feng G, Pendergast A. Postsynaptic requirement for Abl kinases in assembly of the neuromuscular junction. Nat Neurosci. 2003;6:717-23 pubmed
product information
brand :
R&D Systems
master code :
550-AG
SKU :
550-AG-100
product name :
Recombinant Rat Agrin Protein
unit size :
100 ug
seo description :
The Recombinant Rat Agrin Protein from R&D Systems is derived from Sf 21 (baculovirus). The Recombinant Rat Agrin Protein has been validated for the following applications: Bioactivity.
target :
Agrin
category :
Proteins and Enzymes
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with BSA as a carrier protein.
conjugate :
Unconjugated
dilution :
Bioactivity
purity :
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.
species :
Rat
observed molecular weight :
100 kDa, reducing conditions
theoretical molecular weight :
90 kDa
gene symbol :
Agrn
details of functionality :
Measured by its ability to induce acetylcholine receptor clustering on myotubes differentiated from C2C12 mouse myoblast cells. Ferns, M.J. et al . (1993) Neuron 11 :491. The ED50 for this effect is 2-6 ng/mL. Measured by its binding ability in a functional ELISA. When recombinant human LRP-4 is coated at 0.5 ╡g/mL, Recombinant Rat Agrin binds with an apparent Kd 3 nM.
endotoxin note :
<0.10 EU per 1 ╡g of the protein by the LAL method.
accessionNumbers :
P25304.2
applications :
Bioactivity
source :
Sf 21 (baculovirus)-derived Recombinant Rat Agrin Protein
USD :
498 USD
alt names :
agrin, agrin proteoglycan, AGRN
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.