Recombinant Rat Agrin Protein, CF | R&D Systems 550-AG-100/CF product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Rat Agrin Protein, CF

catalog :

550-AG-100/CF

quantity :

100 ug

price :

498 USD

citations: 23

Reference
Gros K, Matkovi x10d U, Parato G, Mi x161 K, Luin E, Bernareggi A, et al. Neuronal Agrin Promotes Proliferation of Primary Human Myoblasts in an Age-Dependent Manner. Int J Mol Sci. 2022;23: pubmed publisher
Klockner I, Schütt C, Gerhardt T, Boettger T, Braun T. Control of CRK-RAC1 activity by the miR-1/206/133 miRNA family is essential for neuromuscular junction function. Nat Commun. 2022;13:3180 pubmed publisher
Shipton O, Tang C, Paulsen O, Vargas Caballero M. Differential vulnerability of hippocampal CA3-CA1 synapses to Aβ. Acta Neuropathol Commun. 2022;10:45 pubmed publisher
Yamamotoya T, Hasei S, Akasaka Y, Ohata Y, Nakatsu Y, Kanna M, et al. Involvement of neuronal and muscular Trk-fused gene (TFG) defects in the development of neurodegenerative diseases. Sci Rep. 2022;12:1966 pubmed publisher
Pinto C, Perez V, Mella J, Albistur M, Caprile T, Bronfman F, et al. Transport and Secretion of the Wnt3 Ligand by Motor Neuron-like Cells and Developing Motor Neurons. Biomolecules. 2021;11: pubmed publisher
Oury J, Zhang W, Leloup N, Koide A, Corrado A, Ketavarapu G, et al. Mechanism of disease and therapeutic rescue of Dok7 congenital myasthenia. Nature. 2021;595:404-408 pubmed publisher
Proietti D, Giordani L, De Bardi M, D Ercole C, Lozanoska Ochser B, Amadio S, et al. Activation of skeletal muscle-resident glial cells upon nerve injury. JCI Insight. 2021;6: pubmed publisher
Oentaryo M, Tse A, Lee C. Neuronal MT1-MMP mediates ECM clearance and Lrp4 cleavage for agrin deposition and signaling in presynaptic development. J Cell Sci. 2020;133: pubmed publisher
Chen P, Zelada D, Belhasan D, Akaaboune M. Phosphorylation of α-dystrobrevin is essential for αkap accumulation and acetylcholine receptor stability. J Biol Chem. 2020;295:10677-10688 pubmed publisher
Xing G, Jing H, Zhang L, Cao Y, Li L, Zhao K, et al. A mechanism in agrin signaling revealed by a prevalent Rapsyn mutation in congenital myasthenic syndrome. elife. 2019;8: pubmed publisher
Njah K, Chakraborty S, Qiu B, Arumugam S, Raju A, Pobbati A, et al. A Role of Agrin in Maintaining the Stability of Vascular Endothelial Growth Factor Receptor-2 during Tumor Angiogenesis. Cell Rep. 2019;28:949-965.e7 pubmed publisher
Takata K, Stathopoulos P, Cao M, Mané Damas M, Fichtner M, Benotti E, et al. Characterization of pathogenic monoclonal autoantibodies derived from muscle-specific kinase myasthenia gravis patients. JCI Insight. 2019;4: pubmed publisher
Blondelle J, Tallapaka K, Seto J, Ghassemian M, Clark M, Laitila J, et al. Cullin-3 dependent deregulation of ACTN1 represents a new pathogenic mechanism in nemaline myopathy. JCI Insight. 2019;5: pubmed publisher
Maffioletti S, Sarcar S, Henderson A, Mannhardt I, Pinton L, Moyle L, et al. Three-Dimensional Human iPSC-Derived Artificial Skeletal Muscles Model Muscular Dystrophies and Enable Multilineage Tissue Engineering. Cell Rep. 2018;23:899-908 pubmed publisher
Cantor S, Zhang W, Delestrée N, Remédio L, Mentis G, Burden S. Preserving neuromuscular synapses in ALS by stimulating MuSK with a therapeutic agonist antibody. elife. 2018;7: pubmed publisher
Ito K, Ohkawara B, Yagi H, Nakashima H, Tsushima M, Ota K, et al. Lack of Fgf18 causes abnormal clustering of motor nerve terminals at the neuromuscular junction with reduced acetylcholine receptor clusters. Sci Rep. 2018;8:434 pubmed publisher
Blondelle J, Shapiro P, Domenighetti A, Lange S. Cullin E3 Ligase Activity Is Required for Myoblast Differentiation. J Mol Biol. 2017;429:1045-1066 pubmed publisher
Nakashima H, Ohkawara B, Ishigaki S, Fukudome T, Ito K, Tsushima M, et al. R-spondin 2 promotes acetylcholine receptor clustering at the neuromuscular junction via Lgr5. Sci Rep. 2016;6:28512 pubmed publisher
Chen Y, Ip F, Shi L, Zhang Z, Tang H, Ng Y, et al. Coronin 6 regulates acetylcholine receptor clustering through modulating receptor anchorage to actin cytoskeleton. J Neurosci. 2014;34:2413-21 pubmed publisher
Ohkawara B, Cabrera Serrano M, Nakata T, Milone M, Asai N, Ito K, et al. LRP4 third β-propeller domain mutations cause novel congenital myasthenia by compromising agrin-mediated MuSK signaling in a position-specific manner. Hum Mol Genet. 2014;23:1856-68 pubmed publisher
Choi H, Liu Y, Tennert C, Sugiura Y, Karakatsani A, Kroger S, et al. APP interacts with LRP4 and agrin to coordinate the development of the neuromuscular junction in mice. elife. 2013;2:e00220 pubmed publisher
Xu X, Fu A, Ip F, Wu C, Duan S, Poo M, et al. Agrin regulates growth cone turning of Xenopus spinal motoneurons. Development. 2005;132:4309-16 pubmed
Finn A, Feng G, Pendergast A. Postsynaptic requirement for Abl kinases in assembly of the neuromuscular junction. Nat Neurosci. 2003;6:717-23 pubmed

product information

master code :

550-AG/CF

SKU :

550-AG-100/CF

product name :

Recombinant Rat Agrin Protein, CF

unit size :

100 ug

description :

The Recombinant Rat Agrin Protein, CF from R&D Systems is derived from Sf 21 (baculovirus). The Recombinant Rat Agrin Protein, CF has been validated for the following applications: Bioactivity.

target :

Agrin

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS.

conjugate :

Unconjugated

purity :

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Rat

observed molecular weight :

100 kDa, reducing conditions

theoretical molecular weight :

90 kDa

gene symbol :

Agrn

details of functionality :

Measured by its ability to induce acetylcholine receptor clustering on myotubes differentiated from C2C12 mouse myoblast cells. Ferns, M.J. et al . (1993) Neuron 11 :491. The ED50 for this effect is 2-6 ng/mL. Measured by its binding ability in a functional ELISA. When recombinant human LRP-4 is coated at 0.5 ╡g/mL, Recombinant Rat Agrin binds with an apparent Kd 3 nM.

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

P25304.2

applications :

Bioactivity

source long :

Spodoptera frugiperda , Sf 21 (baculovirus)-derived rat Agrin protein Ala1153-Pro1959 (Pro1788-Ser1798 del), with an N-terminal Met and 6-His tag

source short :

Sf 21 (baculovirus)

USD :

498 USD

alt names :

agrin, agrin proteoglycan, AGRN

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.