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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human Dkk-1 Protein
catalog :
5439-DK-010
quantity :
10 ug
price :
377 USD
more info or order :
citations: 82
Reference
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Lyros O, Lamprecht A, Nie L, Thieme R, Götzel K, Gasparri M, et al. Dickkopf-1 (DKK1) promotes tumor growth via Akt-phosphorylation and independently of Wnt-axis in Barrett's associated esophageal adenocarcinoma. Am J Cancer Res. 2019;9:330-346 pubmed
Pérez García S, Carrión M, Villanueva Romero R, Hermida Gómez T, Fernández Moreno M, Mellado M, et al. Wnt and RUNX2 mediate cartilage breakdown by osteoarthritis synovial fibroblast-derived ADAMTS-7 and -12. J Cell Mol Med. 2019;23:3974-3983 pubmed publisher
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Pontremoli M, Brioschi M, Baetta R, Ghilardi S, Banfi C. Identification of DKK-1 as a novel mediator of statin effects in human endothelial cells. Sci Rep. 2018;8:16671 pubmed publisher
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Chen Y, Tsai C, Wei Y, Wu Y, Hsu W, Lin H, et al. ATOH1/RFX1/RFX3 transcription factors facilitate the differentiation and characterisation of inner ear hair cell-like cells from patient-specific induced pluripotent stem cells harbouring A8344G mutation of mitochondrial DNA. Cell Death Dis. 2018;9:437 pubmed publisher
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Kokabu S, Rosen V. BMP3 expression by osteoblast lineage cells is regulated by canonical Wnt signaling. FEBS Open Bio. 2018;8:168-176 pubmed publisher
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Liu T, Zhang L, Wang Y, Zhang H, Li L, Bao X. Dickkopf-1 inhibits Wnt3a-induced migration and epithelial-mesenchymal transition of human lens epithelial cells. Exp Eye Res. 2017;161:43-51 pubmed publisher
Kramer N, Schmöllerl J, Unger C, Nivarthi H, Rudisch A, Unterleuthner D, et al. Autocrine WNT2 signaling in fibroblasts promotes colorectal cancer progression. Oncogene. 2017;36:5460-5472 pubmed publisher
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Gao Y, Cai A, Xi H, Li J, Xu W, Zhang Y, et al. Ring finger protein 43 associates with gastric cancer progression and attenuates the stemness of gastric cancer stem-like cells via the Wnt-?/catenin signaling pathway. Stem Cell Res Ther. 2017;8:98 pubmed publisher
Ou F, Su K, Sun J, Liao W, Yao Y, Zheng Y, et al. The LncRNA ZBED3-AS1 induces chondrogenesis of human synovial fluid mesenchymal stem cells. Biochem Biophys Res Commun. 2017;487:457-463 pubmed publisher
Chang K, Hertz J, Zhang X, Jin X, Shaw P, Derosa B, et al. Novel Regulatory Mechanisms for the SoxC Transcriptional Network Required for Visual Pathway Development. J Neurosci. 2017;37:4967-4981 pubmed publisher
Jeong H, Kwack M, Kim M, Kim J, Sung Y. Attenuation of Dickkopf 1-Induced Hair Growth Inhibition in Cultured Human Hair Follicles by Tianeptine. Ann Dermatol. 2017;29:102-105 pubmed publisher
Shao J, Zhou P, Peng G. Experimental Study of the Biological Properties of Human Embryonic Stem Cell-Derived Retinal Progenitor Cells. Sci Rep. 2017;7:42363 pubmed publisher
Gibson A, Hui Mingalone C, Foote A, Uchimura T, Zhang M, Zeng L. Wnt7a Inhibits IL-1β Induced Catabolic Gene Expression and Prevents Articular Cartilage Damage in Experimental Osteoarthritis. Sci Rep. 2017;7:41823 pubmed publisher
Chang Y, Chu T, Ding D. WNT/β-Catenin signaling pathway regulates non-tumorigenesis of human embryonic stem cells co-cultured with human umbilical cord mesenchymal stem cells. Sci Rep. 2017;7:41913 pubmed publisher
Du J, Yanagida A, Knight K, Engel A, Vo A, Jankowski C, et al. Reductive carboxylation is a major metabolic pathway in the retinal pigment epithelium. Proc Natl Acad Sci U S A. 2016;113:14710-14715 pubmed publisher
Schwafertz C, Schinner S, Kühn M, Haase M, Asmus A, Mülders Opgenoorth B, et al. Endothelial cells regulate ?-catenin activity in adrenocortical cells via secretion of basic fibroblast growth factor. Mol Cell Endocrinol. 2017;441:108-115 pubmed publisher
Junker S, Frommer K, Krumbholz G, Tsiklauri L, Gerstberger R, Rehart S, et al. Expression of adipokines in osteoarthritis osteophytes and their effect on osteoblasts. Matrix Biol. 2017;62:75-91 pubmed publisher
Majumder M, Xin X, Liu L, Tutunea Fatan E, Rodriguez Torres M, Vincent K, et al. COX-2 Induces Breast Cancer Stem Cells via EP4/PI3K/AKT/NOTCH/WNT Axis. Stem Cells. 2016;34:2290-305 pubmed publisher
Carthy J, Engström U, Heldin C, Moustakas A. Commercially Available Preparations of Recombinant Wnt3a Contain Non-Wnt Related Activities Which May Activate TGF-β Signaling. J Cell Biochem. 2016;117:938-45 pubmed publisher
Denicol A, Leão B, Dobbs K, Mingoti G, Hansen P. Influence of Sex on Basal and Dickkopf-1 Regulated Gene Expression in the Bovine Morula. PLoS ONE. 2015;10:e0133587 pubmed publisher
Xue J, Chen Y, Wu Y, Wang Z, Zhou A, Zhang S, et al. Tumour suppressor TRIM33 targets nuclear β-catenin degradation. Nat Commun. 2015;6:6156 pubmed publisher
Viale Bouroncle S, Klingelhöffer C, Ettl T, Reichert T, Morsczeck C. A protein kinase A (PKA)/β-catenin pathway sustains the BMP2/DLX3-induced osteogenic differentiation in dental follicle cells (DFCs). Cell Signal. 2015;27:598-605 pubmed publisher
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Tsai H, Deng W, Lai W, Chiu W, Yang C, Tsai Y, et al. Wnts enhance neurotrophin-induced neuronal differentiation in adult bone-marrow-derived mesenchymal stem cells via canonical and noncanonical signaling pathways. PLoS ONE. 2014;9:e104937 pubmed publisher
Chen C, Zhou H, Zhang X, Ma X, Liu Z, Liu X. Elevated levels of Dickkopf-1 are associated with β-catenin accumulation and poor prognosis in patients with chondrosarcoma. PLoS ONE. 2014;9:e105414 pubmed publisher
Peng X, Yang L, Chang H, Dai G, Wang F, Duan X, et al. Wnt/?-catenin signaling regulates the proliferation and differentiation of mesenchymal progenitor cells through the p53 pathway. PLoS ONE. 2014;9:e97283 pubmed publisher
Burnight E, Wiley L, Drack A, Braun T, Anfinson K, Kaalberg E, et al. CEP290 gene transfer rescues Leber congenital amaurosis cellular phenotype. Gene Ther. 2014;21:662-72 pubmed publisher
Ronaghi M, Nasr M, Ealy M, Durruthy Durruthy R, Waldhaus J, Diaz G, et al. Inner ear hair cell-like cells from human embryonic stem cells. Stem Cells Dev. 2014;23:1275-84 pubmed publisher
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Liang P, Lan F, Sanchez Freire V, Simmons C, Gong T, Sharma A, et al. Screening drug-induced arrhythmia [corrected] using human induced pluripotent stem cell-derived cardiomyocytes and low-impedance microelectrode arrays. Circulation. 2013;128:S3-13 pubmed publisher
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Rasmussen N, Wright T, Brooks S, Hacker K, Debebe Z, Sendor A, et al. Receptor tyrosine kinase-like orphan receptor 2 (Ror2) expression creates a poised state of Wnt signaling in renal cancer. J Biol Chem. 2013;288:26301-10 pubmed publisher
Maroof A, Keros S, Tyson J, Ying S, Ganat Y, Merkle F, et al. Directed differentiation and functional maturation of cortical interneurons from human embryonic stem cells. Cell Stem Cell. 2013;12:559-72 pubmed publisher
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Liang P, Lan F, Lee A, Gong T, Sanchez Freire V, Wang Y, et al. Drug screening using a library of human induced pluripotent stem cell-derived cardiomyocytes reveals disease-specific patterns of cardiotoxicity. Circulation. 2013;127:1677-91 pubmed publisher
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Kwack M, Kim M, Kim J, Sung Y. Dickkopf 1 promotes regression of hair follicles. J Invest Dermatol. 2012;132:1554-60 pubmed publisher
Scheel C, Eaton E, Li S, Chaffer C, Reinhardt F, Kah K, et al. Paracrine and autocrine signals induce and maintain mesenchymal and stem cell states in the breast. Cell. 2011;145:926-40 pubmed publisher
Tomaselli S, Megiorni F, Lin L, Mazzilli M, Gerrelli D, Majore S, et al. Human RSPO1/R-spondin1 is expressed during early ovary development and augments β-catenin signaling. PLoS ONE. 2011;6:e16366 pubmed publisher
Chua A, Ma D, Gan S, Fu Z, Han H, Song C, et al. The role of R-spondin2 in keratinocyte proliferation and epidermal thickening in keloid scarring. J Invest Dermatol. 2011;131:644-54 pubmed publisher
Zhang Q, Jiang J, Han P, Yuan Q, Zhang J, Zhang X, et al. Direct differentiation of atrial and ventricular myocytes from human embryonic stem cells by alternating retinoid signals. Cell Res. 2011;21:579-87 pubmed publisher
Granchi D, Corrias M, Garaventa A, Baglìo S, Cangemi G, Carlini B, et al. Neuroblastoma and bone metastases: clinical significance and prognostic value of Dickkopf 1 plasma levels. Bone. 2011;48:152-9 pubmed publisher
Fasano C, Chambers S, Lee G, Tomishima M, Studer L. Efficient derivation of functional floor plate tissue from human embryonic stem cells. Cell Stem Cell. 2010;6:336-47 pubmed publisher
Koch S, Capaldo C, Samarin S, Nava P, Neumaier I, Skerra A, et al. Dkk-1 inhibits intestinal epithelial cell migration by attenuating directional polarization of leading edge cells. Mol Biol Cell. 2009;20:4816-25 pubmed publisher
Zenzmaier C, Marksteiner J, Kiefer A, Berger P, Humpel C. Dkk-3 is elevated in CSF and plasma of Alzheimer's disease patients. J Neurochem. 2009;110:653-61 pubmed publisher
Cho S, Yang J, Sun H, Jung J, Her S, Cho H, et al. Wnt inhibitory factor (WIF)-1 inhibits osteoblastic differentiation in mouse embryonic mesenchymal cells. Bone. 2009;44:1069-77 pubmed publisher
image
image 1 :
R&D Systems 5439-DK-010 image 1
Recombinant Human Dkk-1 Protein Bioactivity
product information
brand :
R&D Systems
master code :
5439-DK
SKU :
5439-DK-010
product name :
Recombinant Human Dkk-1 Protein
description :
The Recombinant Human Dkk-1 Protein from R&D Systems is derived from Sf 21 (baculovirus). The Recombinant Human Dkk-1 Protein has been validated for the following applications: Bioactivity.
target :
Dkk-1
category :
Proteins and Enzymes
unit sizes :
10 ug
buffer :
Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
conjugate :
Unconjugated
purity :
>95%, by SDS-PAGE with silver staining.
species :
Human
observed molecular weight :
33-38 kDa, reducing conditions
theoretical molecular weight :
25.8 kDa
gene symbol :
DKK1
details of functionality :
Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. Recombinant Human Dkk-1 (Catalog # 5439-DK) inhibits a constant dose of 500 ng/mL of Recombinant Human Wnt-3a (Catalog # 5036-WN ). The ED50 for this effect is 10-60 ng/mL.
endotoxin note :
<1.0 EU per 1 µg of the protein by the LAL method.
accessionNumbers :
O94907
applications :
Bioactivity
source :
Sf 21 (baculovirus)-derived Recombinant Human Dkk-1 Protein
USD :
377 USD
alt names :
dickkopf (Xenopus laevis) homolog 1, dickkopf homolog 1 (Xenopus laevis), dickkopf related protein-1, Dickkopf-1, dickkopf-related protein 1, Dkk1, DKK-1, hDkk-1, SKdickkopf-1 like
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.